ADRB2 Antibody

ADRB2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to ADRB2.

The ADRB2 Protein, belonging to the beta-adrenergic receptor family, plays a pivotal role in mediating catecholamine-induced activation of adenylate cyclase through G protein action. With approximately 30-fold higher affinity for epinephrine compared to norepinephrine, ADRB2 is a key player in cellular responses to sympathetic nervous system stimulation. The protein engages in various molecular interactions, binding to NHERF1, GPRASP1, ARRB1, ARRB2, SRC, USP20, USP33, VHL, EGLN3, SNX27, CNIH4, ARRDC3, NEDD4, and MARCHF2. These interactions contribute to the intricate regulation of ADRB2 function, including its ubiquitination, degradation, and recycling processes, underscoring its importance in modulating cellular responses to adrenergic signaling.

P07550

Predicted band size: 46 kDa; Observed band size: 50 kDa

affinity purified

Cell membrane; Multi-pass membrane protein. Note=Colocalizes with VHL at the cell membrane.

Non-conjugated

Unmodified

AB_3103200

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

Dog, Rabbit

WB: 1:500-2000

• 
  Western blot analysis of extracts from M-liver (lane 1) and M-heart (lane 2) and CaCO2 (lane 3) and M-intestine (lane 4) and THP-1 (lane 5) using ADRB2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human ADRB2: 201-300/418