Activin Receptor Type IIB Antibody (YA2345)

Activin Receptor Type IIB Antibody (YA2345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Activin Receptor Type IIB.

Activin Receptor Type IIB (ACVR2B) is a transmembrane serine/threonine kinase that forms an activin receptor complex with type I receptors (ACVR1, ACVR1B or ACVR1C). Functioning as the primary receptor for activin-A/INHBA, activin-B/INHBB and inhibin-A/INHA-INHBA, it regulates diverse processes including neuronal differentiation/survival, hair follicle cycling, pituitary FSH production, wound healing, extracellular matrix formation, immunosuppression and carcinogenesis (by similar: potential paracrine/autocrine roles in ovarian folliculogenesis). Upon activation, ACVR2B phosphorylates type I receptors to initiate SMAD2/SMAD3-SMAD4-mediated transcriptional regulation. Its signaling can be inhibited by SMAD7 (recruited via FKBP1A) or inhibin-B (bound through IGSF1 coreceptor).

Q13705

Predicted band size: 58 kDa; Observed band size: 65 kDa

Affinity Purified

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000

• 
  Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), K562 (lane 2, 15 μg), Hela (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 293 (lane 5, 15 μg) using Activin Receptor Type IIB Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Activin Receptor Type IIB aa150-200.