A1BG Antibody (YA4716)

A1BG Antibody (YA4716) is a mouse-derived and non-conjugated monoclonal antibody, targeting to A1BG. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human.

Alpha-1-B glycoprotein (A1BG) is a human plasma glycoprotein of unknown function, belonging to the immunoglobulin (Ig) superfamily, which contains 5 repeating immunoglobulin-like structural domains (each about 92 to 98 amino acids) with sequence similarity to the variable region of members of the Ig superfamily; the Ig superfamily serves diverse functions based on molecular recognition especially in the immune system and cell adhesion. A1BG inhibits sterol-binding and export by cysteine-rich secretory protein 2 (CRISP2), with its interaction with CRISP2 occurring in the third of its five repeated immunoglobulin-like domains and requiring magnesium, suggesting that coordination of Mg²⁺ by the highly conserved tetrad residues within the cysteine-rich secretory protein (CRISP) domain is essential for a stable interaction between the two proteins; coexpression with CRISP proteins abolishes their sterol export function in yeast, and their interaction inhibits sterol-binding in vitro. In addition, A1BG is an autoantigen in patients with rheumatoid arthritis and is overexpressed in rheumatoid arthritis, pancreatic ductal adenocarcinoma, bladder, breast and lung cancer, pediatric patients with steroid resistant nephrotic syndrome, and liver cancer cell lines, suggesting it might be a cancer associated gene and a novel tumor marker; its glycosylation pattern is suggested to play a crucial role in its functional diversity, influencing interactions with other proteins and cellular compartments. Meanwhile, A1BG binds CRISP3 with nanomolar affinity, forming a stable, noncovalent, equimolar stoichiometric complex, and serum A1BG across different mammals (including cow, horse, and rabbit) all bind human CRISP3, suggesting potential functional significance.

P04217

Predicted band size: 54 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human A1BG expressed in E. Coli.