(-)-Alkannin

Name: (-)-Alkannin
Brand: MedChemExpress
SKU: HY-N6012
Rating: 5.0 (2 reviews)

Cat. No.: HY-N6012 Purity: 99.88%: Data Sheet
COA

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Handling Instructions
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(-)-Alkannin, found in Alkanna tinctoria, is used as a food coloring. (-)-Alkannin shows anticancer activity, arrests cell cycle, and induces apoptosis. (-)-Alkannin improves hepatic inflammation in a Rho-kinase pathway.

For research use only. We do not sell to patients.

(-)-Alkannin Chemical Structure

CAS No. : 517-88-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of (-)-Alkannin:

Shikonin In-stock
Alkannin Get quote

2 Publications Citing Use of MCE (-)-Alkannin

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
AU565	IC₅₀	0.9 μM Compound: 4	Cytotoxicity against human AU565 cells after 48 hrs by MTT assay Cytotoxicity against human AU565 cells after 48 hrs by MTT assay	[PMID: 20405844]
DU-145	IC₅₀	19.9 μM Compound: Alkannin	Anticancer activity against human DU-145 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Anticancer activity against human DU-145 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35367708]
DU-145	IC₅₀	19.9 μM Compound: AK	Cytotoxicity against human DU145 cells by MTT assay Cytotoxicity against human DU145 cells by MTT assay	[PMID: 25127868]
DU-145	IC₅₀	5.02 μM Compound: 4	Cytotoxicity against human DU145 cells after 48 hrs by MTT assay Cytotoxicity against human DU145 cells after 48 hrs by MTT assay	[PMID: 20405844]
Fibroblast	IC₅₀	1.5 μM Compound: Alkannin	Cytotoxicity against human skin fibroblast cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human skin fibroblast cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35367708]
Fibroblast	IC₅₀	1.5 μM Compound: AK	Cytotoxicity against human skin fibroblast cells by MTT assay Cytotoxicity against human skin fibroblast cells by MTT assay	[PMID: 25127868]
Fibroblast	IC₅₀	1.8 μM Compound: Alkannin	Cytotoxicity against human skin fibroblast cells incubated for 24 hrs by MTT assay Cytotoxicity against human skin fibroblast cells incubated for 24 hrs by MTT assay	[PMID: 35367708]
HCT-15	IC₅₀	1.11 μM Compound: Alkannin	Anticancer activity against human HCT-15 cells assessed as reduction in cell viability for 72 hrs by MTT assay Anticancer activity against human HCT-15 cells assessed as reduction in cell viability for 72 hrs by MTT assay	[PMID: 35367708]
HeLa	IC₅₀	0.91 μM Compound: 4	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 20405844]
Hep 3B2	IC₅₀	3.84 μM Compound: 4	Cytotoxicity against human Hep3B cells after 48 hrs by MTT assay Cytotoxicity against human Hep3B cells after 48 hrs by MTT assay	[PMID: 20405844]
HepG2	IC₅₀	3.8 μM Compound: 4	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 20405844]
HT-29	IC₅₀	4.03 μM Compound: 4	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 20405844]
K562	IC₅₀	1.3 μM Compound: Alkannin	Anticancer activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Anticancer activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35367708]
K562	IC₅₀	1.3 μM Compound: AK	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 25127868]
K562	IC₅₀	1.5 μM Compound: Alkannin	Anticancer activity against human K562 cells assessed as reduction in cell viability for 72 hrs by MTT assay Anticancer activity against human K562 cells assessed as reduction in cell viability for 72 hrs by MTT assay	[PMID: 35367708]
LNCaP	IC₅₀	10.74 μM Compound: 4	Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay	[PMID: 20405844]
MCF7	IC₅₀	2.4 μM Compound: Alkannin	Anticancer activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Anticancer activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 35367708]
MCF7	IC₅₀	2.4 μM Compound: AK	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 25127868]
MCF7	IC₅₀	2.62 μM Compound: Alkannin	Anticancer activity against human MCF7 cells assessed as reduction in cell viability for 72 hrs by MTT assay Anticancer activity against human MCF7 cells assessed as reduction in cell viability for 72 hrs by MTT assay	[PMID: 35367708]
MCF7	IC₅₀	3.06 μM Compound: 4	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 20405844]
MDA-MB-231	IC₅₀	4.27 μM Compound: 4	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 20405844]
NIH3T3	IC₅₀	5.21 μM Compound: 4	Cytotoxicity against mouse 3T3 cells after 48 hrs by MTT assay Cytotoxicity against mouse 3T3 cells after 48 hrs by MTT assay	[PMID: 20405844]
PC-3	IC₅₀	3.25 μM Compound: 4	Cytotoxicity against human PC3 cells after 48 hrs by MTT assay Cytotoxicity against human PC3 cells after 48 hrs by MTT assay	[PMID: 20405844]
SAOS-2	IC₅₀	2.31 μM Compound: 4	Cytotoxicity against human Saos2 cells after 48 hrs by MTT assay Cytotoxicity against human Saos2 cells after 48 hrs by MTT assay	[PMID: 20405844]
SK-BR-3	IC₅₀	0.78 μM Compound: 4	Cytotoxicity against human SKBR3 cells after 48 hrs by MTT assay Cytotoxicity against human SKBR3 cells after 48 hrs by MTT assay	[PMID: 20405844]
Vero	IC₅₀	0.7 μM Compound: 4	Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay	[PMID: 20405844]

In Vitro

(-)-Alkannin inhibits HCT-116 and SW-480 cells growth with IC₅₀s of 2.38 and 4.53 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

288.30

Formula

C₁₆H₁₆O₅

CAS No.

517-88-4

Appearance

Solid

Color

Brown to red

SMILES

OC1=C2C(C(C=C([C@H](C/C=C(C)/C)O)C2=O)=O)=C(O)C=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (115.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4686 mL	17.3430 mL	34.6861 mL
5 mM	0.6937 mL	3.4686 mL	6.9372 mL
10 mM	0.3469 mL	1.7343 mL	3.4686 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (254 KB) RP-HPLC (273 KB)

Handling Instructions (2659 KB)

References

[1]. Hosokawa Y, et al. Alkannin inhibits CCL3 and CCL5 production in human periodontal ligament cells. Cell Biol Int. 2016 Dec;40(12):1380-1385. [Content Brief]

[2]. Huu Tung N, et al. Naphthoquinone components from Alkanna tinctoria (L.) Tausch show significant antiproliferative effects on human colorectal cancer cells. Phytother Res. 2013 Jan;27(1):66-70. [Content Brief]

[3]. Xue W, et al. Alkannin Inhibited Hepatic Inflammation in Diabetic Db/Db Mice. Cell Physiol Biochem. 2018;45(6):2461-2470. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4686 mL	17.3430 mL	34.6861 mL	86.7152 mL
	5 mM	0.6937 mL	3.4686 mL	6.9372 mL	17.3430 mL
	10 mM	0.3469 mL	1.7343 mL	3.4686 mL	8.6715 mL
	15 mM	0.2312 mL	1.1562 mL	2.3124 mL	5.7810 mL
	20 mM	0.1734 mL	0.8672 mL	1.7343 mL	4.3358 mL
	25 mM	0.1387 mL	0.6937 mL	1.3874 mL	3.4686 mL
	30 mM	0.1156 mL	0.5781 mL	1.1562 mL	2.8905 mL
	40 mM	0.0867 mL	0.4336 mL	0.8672 mL	2.1679 mL
	50 mM	0.0694 mL	0.3469 mL	0.6937 mL	1.7343 mL
	60 mM	0.0578 mL	0.2891 mL	0.5781 mL	1.4453 mL
	80 mM	0.0434 mL	0.2168 mL	0.4336 mL	1.0839 mL
	100 mM	0.0347 mL	0.1734 mL	0.3469 mL	0.8672 mL

(-)-Alkannin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(-)-Alkannin517-88-4ApoptosisInhibitorinhibitorinhibit

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(-)-Alkannin

Customer Review

Other Forms of (-)-Alkannin:

(-)-Alkannin Related Antibodies

2 Publications Citing Use of MCE (-)-Alkannin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

(-)-Alkannin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(-)-Alkannin Related Classifications

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