2. Anti-infection
  3. Virus Protease
  4. AG-7404

AG-7404 is an orally active, irreversible inhibitor of the picornavirus 3C protease. AG-7404 blocks the processing of viral polyproteins, thereby inhibiting viral replication. AG-7404 has synergistic antiviral activity with capsid inhibitors such as V-073 (HY-104074) or BTA798 (HY-106254) and is effective against V-073-resistant variants. AG-7404 is used in the study of enterovirus infections such as poliovirus.

For research use only. We do not sell to patients.

AG-7404 Chemical Structure

AG-7404 Chemical Structure

CAS No. : 343565-99-1

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AG-7404 Related Antibodies

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Description

AG-7404 is an orally active, irreversible inhibitor of the picornavirus 3C protease. AG-7404 blocks the processing of viral polyproteins, thereby inhibiting viral replication. AG-7404 has synergistic antiviral activity with capsid inhibitors such as V-073 (HY-104074) or BTA798 (HY-106254) and is effective against V-073-resistant variants. AG-7404 is used in the study of enterovirus infections such as poliovirus[1][2][3].

In Vitro

AG-7404 (0.080-0.674 μM; 3 d) inhibits viral replication in HeLa cells with poliovirus with an EC50 of 0.080-0.674 μM[1].
AG-7404 (0.218-0.819 μM; 3 d) remains active in V-073-resistant poliovirus strains with an EC50 of 0.218-0.819 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AG-7404 Related Antibodies

Cell Viability Assay[1]

Cell Line: HeLa cells (for wild-type and vaccine-derived poliovirus strains)
Concentration: 0.080-0.674 μM
Incubation Time: 3 days
Result: Dose-dependently reduced viral cytopathic effect (CPE) as assessed by crystal violet staining and CTG assay.
EC50 values were determined for each strain, demonstrating broad activity against wild-type, circulating vaccine-derived (cVDPV), and immunodeficient vaccine-derived (iVDPV) strains.
Molecular Weight

523.54

Formula

C26H29N5O7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NCC1)[C@@H]1C[C@@H](/C=C/C(OCC)=O)NC([C@@H](N2C(C(NC(C3=NOC(C)=C3)=O)=CC=C2)=O)CC#C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (47.75 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9101 mL 9.5504 mL 19.1007 mL
5 mM 0.3820 mL 1.9101 mL 3.8201 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 97.79%

SDS (252 KB)

COA (252 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9101 mL 9.5504 mL 19.1007 mL 47.7518 mL
5 mM 0.3820 mL 1.9101 mL 3.8201 mL 9.5504 mL
10 mM 0.1910 mL 0.9550 mL 1.9101 mL 4.7752 mL
15 mM 0.1273 mL 0.6367 mL 1.2734 mL 3.1835 mL
20 mM 0.0955 mL 0.4775 mL 0.9550 mL 2.3876 mL
25 mM 0.0764 mL 0.3820 mL 0.7640 mL 1.9101 mL
30 mM 0.0637 mL 0.3183 mL 0.6367 mL 1.5917 mL
40 mM 0.0478 mL 0.2388 mL 0.4775 mL 1.1938 mL
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AG-7404 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AG-7404343565-99-1AG7404AG 7404Virus ProteaseInhibitorinhibitorinhibit

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