2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. AF615

AF615 is a small molecule inhibitor targeting the CDT1/Geminin protein complex (IC50 = 0.313 μM). AF615 selectively induces DNA damage, inhibits DNA synthesis, causes cell cycle arrest, and decreases the survival rate in cancer cell lines. AF615 displayed a Ki = 0.37 μM in case of Geminin-tCDT1 interaction and a Ki = 0.75 μM in case of Geminin-miniCDT1 interaction.

For research use only. We do not sell to patients.

AF615

AF615 Chemical Structure

CAS No. : 122510-61-6

Size Price Stock Quantity
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

AF615 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

AF615 is a small molecule inhibitor targeting the CDT1/Geminin protein complex (IC50 = 0.313 μM). AF615 selectively induces DNA damage, inhibits DNA synthesis, causes cell cycle arrest, and decreases the survival rate in cancer cell lines. AF615 displayed a Ki = 0.37 μM in case of Geminin-tCDT1 interaction and a Ki = 0.75 μM in case of Geminin-miniCDT1 interaction[1].

Molecular Weight

267.31

Formula

C10H13N5O2S
CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

O=C(C1=NC=CN=C1)NNC(N2CCOCC2)=S
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 26.74 mg/mL (100.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7410 mL 18.7049 mL 37.4097 mL
5 mM 0.7482 mL 3.7410 mL 7.4819 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7410 mL 18.7049 mL 37.4097 mL 93.5244 mL
5 mM 0.7482 mL 3.7410 mL 7.4819 mL 18.7049 mL
10 mM 0.3741 mL 1.8705 mL 3.7410 mL 9.3524 mL
15 mM 0.2494 mL 1.2470 mL 2.4940 mL 6.2350 mL
20 mM 0.1870 mL 0.9352 mL 1.8705 mL 4.6762 mL
25 mM 0.1496 mL 0.7482 mL 1.4964 mL 3.7410 mL
30 mM 0.1247 mL 0.6235 mL 1.2470 mL 3.1175 mL
40 mM 0.0935 mL 0.4676 mL 0.9352 mL 2.3381 mL
50 mM 0.0748 mL 0.3741 mL 0.7482 mL 1.8705 mL
60 mM 0.0623 mL 0.3117 mL 0.6235 mL 1.5587 mL
80 mM 0.0468 mL 0.2338 mL 0.4676 mL 1.1691 mL
100 mM 0.0374 mL 0.1870 mL 0.3741 mL 0.9352 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

AF615 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AF615122510-61-6AF 615AF-615DNA/RNA SynthesisCDT1/GemininGeminin-tCDT1Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
AF615
Cat. No.:
HY-171180
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Portuguese - PT (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.