Actein

Name: Actein
Brand: MedChemExpress
SKU: HY-N6872
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6872 Purity: 99.25%: Data Sheet
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Actein, a triterpene glycoside, shows an inhibitory effect on cancer cells, which is isolated from the rhizomes of Cimicifuga foetida. Actein suppresses cell proliferation, induces autophagy and apoptosis through promoting ROS/JNK activation, and blunting AKT pathway in bladder cancer. Actein has little toxicity in vivo.

For research use only. We do not sell to patients.

Actein Chemical Structure

CAS No. : 18642-44-9

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Actein:

Actein (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HCC1806	IC₅₀	> 20 μM Compound: 1	Antiproliferative activity against human HCC1806 cells assessed as inhibition of cell growth incubated for 36 hrs by SRB assay Antiproliferative activity against human HCC1806 cells assessed as inhibition of cell growth incubated for 36 hrs by SRB assay	[PMID: 37121522]
HeLa	IC₅₀	> 10 μM Compound: 11	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 25136911]
HL-60	IC₅₀	> 10 μM Compound: 11	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 25136911]
MCF7	IC₅₀	> 10 μM Compound: 11	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 25136911]
MCF7	IC₅₀	14 μM Compound: 1	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 96 hrs by coulter counter analysis Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 96 hrs by coulter counter analysis	[PMID: 37121522]
MDA-MB-231	IC₅₀	> 20 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 36 hrs by SRB assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 36 hrs by SRB assay	[PMID: 37121522]
MDA-MB-231	IC₅₀	74 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells overexpressing ERnegative and Her2 assessed as inhibition of cell growth incubated for 96 hrs by coulter counter analysis Antiproliferative activity against human MDA-MB-231 cells overexpressing ERnegative and Her2 assessed as inhibition of cell growth incubated for 96 hrs by coulter counter analysis	[PMID: 37121522]
NCI-H460	IC₅₀	> 10 μM Compound: 11	Cytotoxicity against human H460 cells after 48 hrs by MTT assay Cytotoxicity against human H460 cells after 48 hrs by MTT assay	[PMID: 25136911]
Oocyte	EC₅₀	36 μM Compound: 1	Allosteric modulation of alpha1beta2gamma2S GABAA receptor expressed in Xenopus laevis oocytes assessed as stimulation of GABA-induced chloride current by electrophysiology Allosteric modulation of alpha1beta2gamma2S GABAA receptor expressed in Xenopus laevis oocytes assessed as stimulation of GABA-induced chloride current by electrophysiology	[PMID: 21082802]
SMMC-7721	IC₅₀	> 10 μM Compound: 11	Cytotoxicity against human SMMC-7721 cells after 48 hrs by MTT assay Cytotoxicity against human SMMC-7721 cells after 48 hrs by MTT assay	[PMID: 25136911]

In Vitro

Actein (0-30 µM, 24 h) significantly suppress cell proliferation and induce cell apoptosis of gastric cancer cells via Caspase-3 and p53 signaling pathway combined with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)^[1].
Actein (0-40 μM, 24 h) suppresses cell proliferation, induces G2/M cell cycle arrest, autophagy and apoptosis in bladder cancer cells^[2].
Actein (0-20 μM, 24 h) potentiates ROS generation and JNK phosphorylation and suppresses AKT pathway in BIU-87 and T24 cells^[2].
Actein (10 nM and 10 μM, 5-10 min) increases calcium release into the cytoplasm of breast cancer cells and colon cancer cells^[3].
Actein (0-40 μg/mL, 48 h) inhibits the NF-κB and MEK pathways by decreasing the level of IKKβ protein in 293T (null) and 293T (NF-κB) cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	SNU-216 and AGS gastric cancer cell lines
Concentration:	0-30 µM
Incubation Time:	24 h
Result:	Exhibited a strong inhibitory effect on gastric cancer cell proliferation in a dose-dependent manner combined with TRAIL in gastric cancer cells.

Immunofluorescence^[2]

Cell Line:	BIU-87 and T24 cells
Concentration:	0-20 μM
Incubation Time:	24 h
Result:	Exhibited dose-increased levels of oxidation-sensitive red fluorescence, indicating the elevation of ROS in BIU-87 and T24 cells.

Western Blot Analysis^[1]

Cell Line:	SNU-216 and AGS gastric cancer cell lines
Concentration:	0-30 µM
Incubation Time:	24 h
Result:	Dose-dependently reduced the AKT/mTOR and JAK2/STAT3 activity, while p38 and JNK were highly phosphorylated in BIU-87 and T24 cells.

Western Blot Analysis^[3]

Cell Line:	293T (null) and 293T (NF-κB) cells
Concentration:	0-40 μg/mL
Incubation Time:	48 h
Result:	Decreased the level of IKKβ protein in a dose dependent manner in 293T (null) and 293T (NF-κB) cells.

Molecular Weight

676.83

Formula

C₃₇H₅₆O₁₁

CAS No.

18642-44-9

Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@@]3([H])[C@@]4(C[C@@H](OC(C)=O)[C@@]1([C@]5([H])[C@](O[C@]6([C@H]7[C@]([C@@H](O)O6)(C)O7)C[C@H]5C)([H])C2)C)[C@@]8([C@@](C(C)([C@@H](O[C@@]9([H])[C@@H]([C@H]([C@H](O)CO9)O)O)CC8)C)([H])CC3)C4

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (147.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4775 mL	7.3874 mL	14.7748 mL
5 mM	0.2955 mL	1.4775 mL	2.9550 mL
10 mM	0.1477 mL	0.7387 mL	1.4775 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.25%

Select Batch:

Data Sheet (281 KB) SDS (252 KB)

COA (254 KB) RP-HPLC (213 KB)

Handling Instructions (2659 KB)

References

[1]. Yang ZC, et al. Actein enhances TRAIL effects on suppressing gastric cancer progression by activating p53/Caspase-3 signaling. Biochem Biophys Res Commun. 2018 Mar 18;497(4):1177-1183. [Content Brief]

[2]. Ji L, et al. Actein induces autophagy and apoptosis in human bladder cancer by potentiating ROS/JNK and inhibiting AKT pathways. Oncotarget. 2017 Nov 1;8(68):112498-112515. [Content Brief]

[3]. Einbond LS, et al. Actein induces calcium release in human breast cancer cells. Fitoterapia. 2013 Dec;91:28-38. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4775 mL	7.3874 mL	14.7748 mL	36.9369 mL
	5 mM	0.2955 mL	1.4775 mL	2.9550 mL	7.3874 mL
	10 mM	0.1477 mL	0.7387 mL	1.4775 mL	3.6937 mL
	15 mM	0.0985 mL	0.4925 mL	0.9850 mL	2.4625 mL
	20 mM	0.0739 mL	0.3694 mL	0.7387 mL	1.8468 mL
	25 mM	0.0591 mL	0.2955 mL	0.5910 mL	1.4775 mL
	30 mM	0.0492 mL	0.2462 mL	0.4925 mL	1.2312 mL
	40 mM	0.0369 mL	0.1847 mL	0.3694 mL	0.9234 mL
	50 mM	0.0295 mL	0.1477 mL	0.2955 mL	0.7387 mL
	60 mM	0.0246 mL	0.1231 mL	0.2462 mL	0.6156 mL
	80 mM	0.0185 mL	0.0923 mL	0.1847 mL	0.4617 mL
	100 mM	0.0148 mL	0.0739 mL	0.1477 mL	0.3694 mL

Actein Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Actein

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Complete Stock Solution Preparation Table

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