2. Immunology/Inflammation
  3. Mucin
  4. Ac5GalNTGc

Ac5GalNTGc is a potent, peracetylated C-2 thioglycolyl-substituted GalNAc analog that efficiently inhibits mucin-type O-glycan biosynthesis. Ac5GalNTGc reduces leukocyte sialyl-Lewis-X expression and inhibits L-/P-selectin mediated rolling under flow, as well as P-selectin dependent leukocyte-platelet adhesion. Ac5GalNTGc exhibits anti-inflammatory activity in a thioglycollate-induced acute peritonitis model. Ac5GalNTGc can be used for studies of O-glycan/mucin biology, inflammation, and related translational research.

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Ac5GalNTGc

Ac5GalNTGc Chemical Structure

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

Other Forms of Ac5GalNTGc:

Ac5GalNTGc Related Antibodies

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1 Publications Citing Use of MCE Ac5GalNTGc

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Description

Ac5GalNTGc is a potent, peracetylated C-2 thioglycolyl-substituted GalNAc analog that efficiently inhibits mucin-type O-glycan biosynthesis. Ac5GalNTGc reduces leukocyte sialyl-Lewis-X expression and inhibits L-/P-selectin mediated rolling under flow, as well as P-selectin dependent leukocyte-platelet adhesion. Ac5GalNTGc exhibits anti-inflammatory activity in a thioglycollate-induced acute peritonitis model. Ac5GalNTGc can be used for studies of O-glycan/mucin biology, inflammation, and related translational research[1][2].

In Vitro

Ac5GalNTGc (0-50 μM, 48 h) drastically reduces CD43-L60, CD43-1G10, CD45-UCHL-1 and CD43-L10 epitopes in Jurkat cells[1].
Ac5GalNTGc (50-80 μM, 40 h) effectively inhibits O-glycan biosynthesis in various cells (HL-60 promyelocytes, breast cancer cells, prostate cancer cells)[2].
Ac5GalNTGc (50 μM, 40 h) significantly reduces the expression of sialyl Lewis-X on the surface of HL-60 cells[2].
Ac5GalNTGc (80 μM, 40 h) reduces rolling of HL-60 cells on L-selectin substrates and on P-selectin-bearing CHO-P cells, without affecting E-selectin-dependent rolling, and also decreases P-selectin-dependent leukocyte-platelet adhesion[2].
Ac5GalNTGc (80 μM, 40 h) reduces the apparent molecular mass of the major L-/P-selectin ligand PSGL-1 in HL-60 cells and also decreases the molecular mass of CD43[2].
Ac5GalNTGc (50-80 μM, 40 h) increases VVA binding in mouse bone marrow cells (mBMCs) and neutrophils (mPMNs), reduces P-selectin-IgG binding and L-selectin binding without affecting PSGL-1 expression[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ac5GalNTGc Related Antibodies
In Vivo

Ac5GalNTGc (100 mg/kg, i.p., once daily, 4 days) exhibits anti-inflammatory activity in a thioglycollate-induced acute peritonitis model of C57BL/6 mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Thioglycollate (4%) was intraperitoneally injected into 8-12 week-old C57BL/6 wild-type mice of either sex to induce acute peritonitis[2]
Dosage: 100 mg/kg
Administration: i.p. once daily for 4 days
Result: Reduced neutrophil migration into the peritoneum in a thioglycollate-induced acute peritonitis model when Ac₅GalNTGc-treated mouse bone marrow cells were injected intravenously.
Decreased neutrophil extravasation into the peritoneal lavage in mice.
Increased VVA binding to neutrophils in the peritoneal lavage by two-fold.
Caused no significant changes in peripheral blood counts and leukocyte differentials after 4 days of administration.
Molecular Weight

463.46

Formula

C18H25NO11S
Appearance

Solid

Color

White to off-white

SMILES

CC(O[C@@H]1[C@H]([C@@H](O[C@@H]([C@@H]1OC(C)=O)COC(C)=O)OC(C)=O)NC(CSC(C)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (215.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1577 mL 10.7884 mL 21.5768 mL
5 mM 0.4315 mL 2.1577 mL 4.3154 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.39%

SDS (251 KB)

COA (254 KB) HNMR (279 KB) RP-HPLC (172 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1577 mL 10.7884 mL 21.5768 mL 53.9421 mL
5 mM 0.4315 mL 2.1577 mL 4.3154 mL 10.7884 mL
10 mM 0.2158 mL 1.0788 mL 2.1577 mL 5.3942 mL
15 mM 0.1438 mL 0.7192 mL 1.4385 mL 3.5961 mL
20 mM 0.1079 mL 0.5394 mL 1.0788 mL 2.6971 mL
25 mM 0.0863 mL 0.4315 mL 0.8631 mL 2.1577 mL
30 mM 0.0719 mL 0.3596 mL 0.7192 mL 1.7981 mL
40 mM 0.0539 mL 0.2697 mL 0.5394 mL 1.3486 mL
50 mM 0.0432 mL 0.2158 mL 0.4315 mL 1.0788 mL
60 mM 0.0360 mL 0.1798 mL 0.3596 mL 0.8990 mL
80 mM 0.0270 mL 0.1349 mL 0.2697 mL 0.6743 mL
100 mM 0.0216 mL 0.1079 mL 0.2158 mL 0.5394 mL
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Ac5GalNTGc Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac5GalNTGcMucinglycosylationO-glycanmucininhibitorneutrophilcell adhesioninflammationsialyl lewis-XselectinInhibitorinhibit

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