2. Metabolic Enzyme/Protease
  3. MAGL
  4. ABD957

ABD957 is a potent and selective covalent inhibitor of the ABHD17 family of depalmitoylases, with an IC50 of 0.21 μM for ABHD17B. ABD957 can block N-Ras signaling and the growth of NRAS-mutant AML cells.

For research use only. We do not sell to patients.

ABD957

ABD957 Chemical Structure

CAS No. : 3007772-60-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
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500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

ABD957 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE ABD957

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

ABD957 is a potent and selective covalent inhibitor of the ABHD17 family of depalmitoylases, with an IC50 of 0.21 μM for ABHD17B. ABD957 can block N-Ras signaling and the growth of NRAS-mutant AML cells[1].

IC50 & Target

IC50: 0.21 µM (ABHD17B)[1]
In Vitro

ABD957 (500 nM; pretreated for 1 h) attenuates N-Ras depalmitoylation in AML cells[1].
ABD957 (0.11 nM-10 μM; 72 h) blocks the signaling and proliferation of NRAS-mutant leukemia cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ABD957 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: OCI-AML3, THP1, HL60, NB-4 and NOMO1 cells
Concentration: 0.1, 1, 10, 100, 1000, 10000 nM
Incubation Time: 72 hours
Result: Reduced the growth of NRAS-mutant AML cell lines (OCI-AML3, THP1 and HL60), but not KRAS mutant cell lines (NB-4 and NOMO1).
Molecular Weight

627.68

Formula

C27H36F3N7O5S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@H]1CN(CCN1C(N2C=CC(S(=O)(N(C)C)=O)=N2)=O)CC3=C(C=C(C=C3)C(F)(F)F)N4C5CCC4CN(C5)CC(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (159.32 mM; ultrasonic and warming and heat to 160°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5932 mL 7.9658 mL 15.9317 mL
5 mM 0.3186 mL 1.5932 mL 3.1863 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 5 mg/mL (7.97 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 5 mg/mL (7.97 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5932 mL 7.9658 mL 15.9317 mL 39.8292 mL
5 mM 0.3186 mL 1.5932 mL 3.1863 mL 7.9658 mL
10 mM 0.1593 mL 0.7966 mL 1.5932 mL 3.9829 mL
15 mM 0.1062 mL 0.5311 mL 1.0621 mL 2.6553 mL
20 mM 0.0797 mL 0.3983 mL 0.7966 mL 1.9915 mL
25 mM 0.0637 mL 0.3186 mL 0.6373 mL 1.5932 mL
30 mM 0.0531 mL 0.2655 mL 0.5311 mL 1.3276 mL
40 mM 0.0398 mL 0.1991 mL 0.3983 mL 0.9957 mL
50 mM 0.0319 mL 0.1593 mL 0.3186 mL 0.7966 mL
60 mM 0.0266 mL 0.1328 mL 0.2655 mL 0.6638 mL
80 mM 0.0199 mL 0.0996 mL 0.1991 mL 0.4979 mL
100 mM 0.0159 mL 0.0797 mL 0.1593 mL 0.3983 mL
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ABD957 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ABD9573007772-60-0ABD 957ABD-957MAGLMonoacylglycerol lipaseABHD17depalmitoylasesN-RasNRASAMLInhibitorinhibitorinhibit

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