AAA-10 formic 

AAA-10 formic is an orally active gut bacterial bile salt hydrolases (BSH) inhibitor, with IC₅₀s of 10 nM, 80 nM against B. theta rBSH and B. longum rBSH, respectively^[1].

IC50: 10 nM (B. theta rBSH), 80 nM (B. longum rBSH)^[1]

AAA-10 (100 μM; 24 h) formic inhibits BSH activity in bacterial cultures, with IC₅₀s of 74 nM, 901 nM for Gram-negative and Gram-positive bacteria, respectively^[1].
AAA-10 (20 μM; 2 h) formic significantly inhibits deconjugation of glycochenodeoxycholic acid-d4 or taurocholic acid-d4 substrates of human feces^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AAA-10 (30 mg/kg; orally gavage daily for 5 days) formic decreases the abundances of deoxycholic acid (DCA) and lithocholic acid (LCA) in mice feces starting in day 2-5^[1].
AAA-10 (30 mg/kg) formic displays high colonic exposure and low gut permeability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

518.68

C₂₆H₄₃FO₇S

C[C@H](CCC(CF)=O)[C@@]1([H])CC[C@@]2([H])[C@]3([H])CC[C@]4([H])C[C@H](OS(=O)(O)=O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C.O=CO

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Adhikari AA, et al. A Gut-Restricted Lithocholic Acid Analog as an Inhibitor of Gut Bacterial Bile Salt Hydrolases. ACS Chem Biol. 2021;16(8):1401-1412.  [Content Brief]