Triacetin (Synonyms: Glyceryl triacetate; 1,2,3-Triacetoxypropane)

Cat. No.: HY-B0896 Purity: 99.58%: Data Sheet
COA

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Handling Instructions
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Triacetin (Glyceryl triacetate) is a synthetic compound that is a triester of glycerol and acetic acid, orally active. Triacetin increases acetate bioavailability in glioma cells. Triacetin induces glioma cell growth arrest and Apoptosis. Triacetin freely crosses the blood brain barrier/plasma membrane. Triacetin increases histone acetylation and enhances Temozolomide (HY-17364) (TMZ) chemotherapeutic efficacy .

For research use only. We do not sell to patients.

Triacetin Chemical Structure

CAS No. : 102-76-1

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

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Other Forms of Triacetin:

Triacetin (Standard) In-stock
Triacetin-d₅ Get quote
Triacetin-d₉ Get quote

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HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Triacetin (0.25%, 24 h) induces growth arrest of HOG, Hs683, U87, U251, OG33, OG35 GBM9, GBM12, GBM34, GBM2, GBM8 and GBM44 cells in vitro^[1].
Triacetin (25 mM, 24 h) causes effective inhibition on the U87MG (human malignant glioma) cell viability^[2].
Triacetin (12.5-25 mM, 24 h) induces clear G2/M cell-cycle arrest in U87MG cells.^[2].
Triacetin (12.5 mM) induces apoptosis in GBM cancer cells^[2].
Triacetin (25 mM, 24 h) decreases the class I and class II HDAC (histone deacetylase) mRNA expression in U87MG cells^[2].
Triacetin (12.5 mM, 24 h) inhibits HDAC-8 activity in U87MG cells^[2].
Triacetin (12.5 mM, 24 h) activates the mTOR complexes downstream genes which play an important role in cancer cell metabolism such as S6K1, mSIN1, Protor 2, and PKCα^[2].
Triacetin (12.5 mM, 24 h) shows an increased tumor suppressor miRs that can inhibit growth, proliferation, invasion, migration, and angiogenesis in U87MG cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:	Established glioma cell lines and primary tumor-derived GSCs.
Concentration:	0.25%
Incubation Time:	24 h
Result:	Induced G0 growth arrest of all glioma cells, except U87, U251 and GBM (glioblastoma) 8 GSCs, without affecting Oli-Neu OPCs (oligodendrocyte progenitor cell line) or astrocytes and promoted NSC (neural stem cells) expansion. The growth reduction of established glioma cell lines and primary tumor-derived GSCs in vitro is not due to the promotion of differentiation.

Cell Cycle Analysis^[2]

Cell Line:	U87 MG cells
Concentration:	12.5, 25 mM
Incubation Time:	24 h
Result:	Induced 12% and 25% of sub-G1 cells and 60% and 79% of G2/M cells.

Apoptosis Analysis^[2]

Cell Line:	GBM cancer cells
Concentration:	12.5 mM
Incubation Time:	24 h
Result:	Increased caspase-3 activity in treated GBM cancer cells.

RT-PCR^[2]

Cell Line:	U87MG cells
Concentration:	25 mM
Incubation Time:	24 h
Result:	Decreased the classI and class II HDAC (histone deacetylase) mRNA expression. Increased expression of S6K1, mSIN1, Protor 2, and PKCα. Increased expression of miR-15b, miR-92, miR-101, miR-155, miR-199, miR-200, miR-223, and slight increase in expression in miR-16 and miR-17.

In Vivo

Triacetin (5.0 g/kg with 10% v/v oral-sweet SF, p.o., daily, 14 days) enhances TMZ (Temozolomide) (HY-17364) chemotherapeutic efficacy in mice engrafted with oligodendroglioma-derived GSCs^[1].
Triacetin (5.0 g/kg with 10% v/v Ora-Sweet SF, p.o., daily, 40days) alone increases survival of mice orthotopically engrafted with GBM-derived GSCs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult (8 week, 24 - 28g) male athymic mice orthotopically engrafted with oligodendroglioma-derived GSC cells ^[1].
Dosage:	5.0 g/kg with 10% v/v oral-sweet SF
Administration:	p.o., daily, 14 days
Result:	Significantly reduced tumor bioluminescence and increased survival but did not reduce end-point tumor volume with treatment of TMZ (Temozolomide) (HY-17364).

Animal Model:	Adult (8 week, 24 - 28g) male athymic mice orthotopically engrafted with GBM-derived GSC cells^[1].
Dosage:	5.0 g/kg with 10% v/v oral-sweet SF
Administration:	p.o., daily, 40 days
Result:	Alone did not alter blood glucose, bioluminescence, or end-point tumor volume; however, increased survival. Significantly increased survival with treatment of TMZ, with two of eight mice never redeveloping measurable flux or displaying histological signs of tumor at study termination.

Molecular Weight

218.20

Formula

C₉H₁₄O₆

CAS No.

102-76-1

Appearance

Liquid (Density: 1.16 g/cm³)

Color

Colorless to light yellow

SMILES

CC(OCC(OC(C)=O)COC(C)=O)=O

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 300 mg/mL (1374.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5830 mL	22.9148 mL	45.8295 mL
5 mM	0.9166 mL	4.5830 mL	9.1659 mL
10 mM	0.4583 mL	2.2915 mL	4.5830 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 7.5 mg/mL (34.37 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 7.5 mg/mL (34.37 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 7.5 mg/mL (34.37 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.58%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (246 KB) GC (232 KB)

Handling Instructions (2659 KB)

References

[1]. Tsen AR, et al. Triacetin-based acetate supplementation as a chemotherapeutic adjuvant therapy in glioma. Int J Cancer. 2014 Mar 15;134(6):1300-10. [Content Brief]

[2]. Mekala JR, et al. N-acetyl l-aspartate and Triacetin modulate tumor suppressor MicroRNA and class I and II HDAC gene expression induce apoptosis in Glioblastoma cancer cells in vitro. Life Sci. 2021 Dec 1;286:120024. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.5830 mL	22.9148 mL	45.8295 mL	114.5738 mL
	5 mM	0.9166 mL	4.5830 mL	9.1659 mL	22.9148 mL
	10 mM	0.4583 mL	2.2915 mL	4.5830 mL	11.4574 mL
	15 mM	0.3055 mL	1.5277 mL	3.0553 mL	7.6383 mL
	20 mM	0.2291 mL	1.1457 mL	2.2915 mL	5.7287 mL
	25 mM	0.1833 mL	0.9166 mL	1.8332 mL	4.5830 mL
	30 mM	0.1528 mL	0.7638 mL	1.5277 mL	3.8191 mL
	40 mM	0.1146 mL	0.5729 mL	1.1457 mL	2.8643 mL
	50 mM	0.0917 mL	0.4583 mL	0.9166 mL	2.2915 mL
	60 mM	0.0764 mL	0.3819 mL	0.7638 mL	1.9096 mL
	80 mM	0.0573 mL	0.2864 mL	0.5729 mL	1.4322 mL
	100 mM	0.0458 mL	0.2291 mL	0.4583 mL	1.1457 mL

Triacetin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Triacetin102-76-1Glyceryl triacetate 1,2,3-TriacetoxypropaneApoptosisHDACHistone deacetylasesmetabolismglioma cellsmiRscancerInhibitorinhibitorinhibit

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