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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-138200
    Cy5 maleimide
    		99.43%
    Cy5 maleimide is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Cy5 maleimide
  • HY-D0505
    Eosin Y
    		99.42%
    Eosin Y (Acid Red 87 free base) is a soluble acid red dye molecule. Eosin Y has a wide application in organic synthesis as a photoredox catalyst.
    Eosin Y
  • HY-101880
    ADHP
    		99.67%
    ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).
    ADHP
  • HY-111330
    Hydroxyphenyl Fluorescein
    		99.91%
    Hydroxyphenyl Fluorescein (HPF) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940). Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm.
    Hydroxyphenyl Fluorescein
  • HY-D0974
    Sulforhodamine B sodium salt
    		99.43%
    Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.
    Sulforhodamine B sodium salt
  • HY-135985
    DCLK1-IN-1
    		99.35%
    DCLK1-IN-1 is a selective, oral bioavailability in vivo-compatible chemical probe of the doublecortin like kinase 1 (DCLK1 kinase) domain. DCLK1-IN-1 inhibits DCLK1 and DCLK2 kinases (IC50: DCLK1=9.5/57.2 nM and DCLK2=31/103 nM in binding and kinase assay, respectively). DCLK1-IN-1 shows low toxicity, and can investigate DCLK1 biology and establish its role in cancer, like DCLK1+ pancreatic ductal adenocarcinoma (PDAC).
    DCLK1-IN-1
  • HY-D1237
    BODIPY 505/515
    		99.77%
    BODIPY505/515 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 505/515 nm.
    BODIPY 505/515
  • HY-D0716
    Fluo-3AM
    		≥99.0%
    Fluo-3 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.
    Fluo-3AM
  • HY-D0903
    1,8-Diazafluoren-9-one
    		99.66%
    1,8-Diazafluoren-9-one (DFO) is a chemical that is used to find fingerprints on porous surfaces. 1,8-Diazafluoren-9-one reacts with amino acids present in the fingerprint to form highly fluorescent derivatives (Ex/Em = ~470/570).
    1,8-Diazafluoren-9-one
  • HY-D0213
    Sudan Black B
    		99.13%
    Sudan Black B, a fat-soluble diazo dye, is a histochemical stain. Sudan Black B can be used for staining of neutral triglycerides and lipids.
    Sudan Black B
  • HY-136886
    IR-820
    IR-820 (New Indocyanine Green) is an infrared blood pool contrast agent. IR-820 also is normally used as a laser and near-infrared dye to detect and quantify diseased tissue in live animals.
    IR-820
  • HY-D2449
    DQ-BSA-RED
    DQ-BSA-Red is a bovine serum albumin labeled with a red fluorescent dye that can be used to detect lysosomal activity. The excitation wavelength and emission wavelength of DQ-BSA-Red are 590 nm and 620 nm, respectively. The BSA molecule in DQ-BSA-Red is labeled with high concentration of red fluorescent dye in multiple sites, which shows high fluorescence self-inhibition. Once DQ-BSA-RED enters the lysosome, DQ-BSA is cleaved by lysosomal proteases, resulting in unquenched and released fluorescent fragments, emitting bright fluorescence. Inactivated lysosomes are unable to degrade the BSA protein and thus have a lower or even no fluorescent signal.
    DQ-BSA-RED
  • HY-D1727
    CellTracker Red CMTPX
    CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm).
    CellTracker Red CMTPX
  • HY-B1025
    Digoxigenin
    		99.70%
    Digoxigenin (DIG) is a steroid. DIG is used for situ hybridization as a labeling molecule probe due to long shelf life and fast detection and high sensitivity of DIG-labeled riboprobes.
    Digoxigenin
  • HY-D1594
    BODIPY TR Cadaverine
    		99.05%
    BODIPY TR Cadaverine, a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A.
    BODIPY TR Cadaverine
  • HY-D0915
    Brilliant Blue FCF
    		≥98.0%
    Brilliant Blue FCF is an aromatic hydrocarbon, a synthetic dye produced from petroleum and used as a colorant for food and other substances. The solution has a maximum absorption at 628 nm.
    Brilliant Blue FCF
  • HY-D1069
    DBCO-Cy3
    		98.00%
    DBCO-Cy3 (DBCO-Sulfo-Cy3) is the derivative of Cyanine3 fluorophore, a pH insensitive from pH (4-10) orange fluorescent dyewith excitation maximum 555 nm and emission maximum of 580nm. DBCO-Cy3 has fast reaction kinetics and good stability, and is productive to use in many standard fluorescent instrumentations. DBCO-Cy3 is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
    DBCO-Cy3
  • HY-D1314
    FAM azide, 6-isomer
    		99.21%
    FAM azide, 6-isomer (6-FAM azide), a fluorescent dye, is a click chemistry reagent containing an azide group. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    FAM azide, 6-isomer
  • HY-D0236
    Congo Red
    Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.
    Congo Red
  • HY-D1913
    FerroOrange
    		98.2%
    FerroOrange is a Fe2+ selective probe with an excitation wavelength of 543 nm and an emission wavelength of 580 nm. FerroOrange emits fluorescence after binding to intracellular Fe2+, thereby achieving specific labeling of Fe2+. FerroOrange is used to detect the content and distribution of Fe2+ in cells.
    FerroOrange
Cat. No. Product Name / Synonyms Application Reactivity