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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-15930
    TMB
    		99.95%
    TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.
    TMB
  • HY-D0027
    7-Amino-4-methylcoumarin
    		99.91%
    7-Amino-4-methylcoumarin belongs to the coumarin class, can be isolated from the endophytic fungus Xylaria sp. and has a broad spectrum of antibacterial activity. 7-Amino-4-methylcoumarin is also commonly used as an important laser dye that emits in the blue region, capable of analyzing glycoprotein monosaccharides and N-linked oligosaccharides, and is also utilized in tissue pathology analysis, enzyme activity measurement, and copper ion detection. The excitation wavelength and emission wavelength are 351 nm and 430 nm, respectively.
    7-Amino-4-methylcoumarin
  • HY-15096
    MKT-077
    		98.05%
    MKT-077 (FJ-776), a highly water-soluble mitochondrial dye, has significant antitumor activity. MKT-077 exhibits low cytotoxicity, and inhibits broad-spectrum human cancer cell lines (colon cancer, breast cancer, pancreatic cancer). MKT-077 inhibits the growth of tumor in nude mice enograft tumor model. Ex/Em=488/543 nm.
    MKT-077
  • HY-D0023
    Pyranine
    		≥98.0%
    Pyranine (HPTS; Solvent Green 7) is a pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu+ ion(λex=450 nm, λem=510 nm).
    Pyranine
  • HY-148242
    BAY-069
    		99.58%
    BAY-069 is a potent branched-chain amino acid transaminases 1 (BCAT1) and BCAT2 inhibitor with IC50 values of 31 nM and 153 nM, respectively. BAY-069 also can be used as a chemical probe. BAY-069 can be used tor research anticancer.
    BAY-069
  • HY-101880
    ADHP
    		99.90%
    ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).
    ADHP
  • HY-D1540
    Cyanine5.5 amine
    		99.28%
    Cyanine5.5 amine (Cy 5.5 amine), a Cy5.5 Analogue, is a near-infrared (NIR) fluorescent dye (Ex=648 nm, Em=710 nm). Cyanine5.5 amine can be used in the preparation of Cy5.5-labeled nanoparticles, which can be tracked and imaged with low fluorescence background using confocal microscopy.
    Cyanine5.5 amine
  • HY-D1063
    IR-780
    IR780 is a prototypic NIR heptamethine cyanine agent, with a high molar extinction coefficient. The maximum excitation wavelength of IR780 was 777-780 nm, and the maximum emission wavelength was 798-823 nm. IR780 can be used in cancer PTT/PDT and imaging.
    IR-780
  • HY-66021
    6-FAM
    		99.72%
    6-FAM (6-Carboxyfluorescein) is an isomer of carboxyfluorescein and is mainly used for sequencing and labeling of nucleic acids.
    6-FAM
  • HY-D1041
    ICG-OSu
    ICG-OSu (ICG NHS ester) is a near-infrared fluorescent agent ICG derivative. ICG-OSu is amine-reactive and has been widely used to design in vivo imaging probes.
    ICG-OSu
  • HY-137805
    Ferrozine
    		99.96%
    Ferrozine is a spectrophotometric reagent for iron ions, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm. Ferrozine-based colorimetric assays can quantify iron in cells
    Ferrozine
  • HY-D1498
    Mag-Fluo-4 AM
    Mag-Fluo-4 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Mag-Fluo-4 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.
    Mag-Fluo-4 AM
  • HY-D0824A
    CY7-SE triethylamine
    		≥98.0%
    CY7-SE triethylamine is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    CY7-SE triethylamine
  • HY-D1727
    CellTracker Red CMTPX
    CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm).
    CellTracker Red CMTPX
  • HY-101897
    Fura-2 AM
    		≥99.0%
    Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ (calcium ion) indicator.
    Fura-2 AM
  • HY-124409
    WSP-1
    		99.03%
    WSP-1 is a selective and rapid-reaction H2S specific fluorescent dye (Ex/Em=465/515 nm). WSP-1 reacts with H2S with the releasing of fluorophore.
    WSP-1
  • HY-D1237
    BODIPY 505/515
    		99.77%
    BODIPY505/515 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 505/515 nm.
    BODIPY 505/515
  • HY-D1612
    BODIPY FL C5-Ceramide
    		99.65%
    The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY Fl C5-Ceramide is a Golgi-specific green fluorescent dye, which can visualise individual cells. Ex/Em= 505 nm/512 nm.
    BODIPY FL C5-Ceramide
  • HY-D0309
    Rhodamine 6G
    		≥98.0%
    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.
    Rhodamine 6G
  • HY-D2449
    DQ-BSA-RED
    DQ-BSA-Red is a bovine serum albumin labeled with a red fluorescent dye that can be used to detect lysosomal activity. The excitation wavelength and emission wavelength of DQ-BSA-Red are 590 nm and 620 nm, respectively. The BSA molecule in DQ-BSA-Red is labeled with high concentration of red fluorescent dye in multiple sites, which shows high fluorescence self-inhibition. Once DQ-BSA-RED enters the lysosome, DQ-BSA is cleaved by lysosomal proteases, resulting in unquenched and released fluorescent fragments, emitting bright fluorescence. Inactivated lysosomes are unable to degrade the BSA protein and thus have a lower or even no fluorescent signal.
    DQ-BSA-RED
Cat. No. Product Name / Synonyms Application Reactivity