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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D0027
    7-Amino-4-methylcoumarin
    		99.91%
    7-Amino-4-methylcoumarin belongs to the coumarin class, can be isolated from the endophytic fungus Xylaria sp. and has a broad spectrum of antibacterial activity. 7-Amino-4-methylcoumarin is also commonly used as an important laser dye that emits in the blue region, capable of analyzing glycoprotein monosaccharides and N-linked oligosaccharides, and is also utilized in tissue pathology analysis, enzyme activity measurement, and copper ion detection. The excitation wavelength and emission wavelength are 351 nm and 430 nm, respectively.
    7-Amino-4-methylcoumarin
  • HY-D1540
    Cyanine5.5 amine
    		99.28%
    Cyanine5.5 amine (Cy 5.5 amine), a Cy5.5 Analogue, is a near-infrared (NIR) fluorescent dye (Ex=648 nm, Em=710 nm). Cyanine5.5 amine can be used in the preparation of Cy5.5-labeled nanoparticles, which can be tracked and imaged with low fluorescence background using confocal microscopy.
    Cyanine5.5 amine
  • HY-158082C
    TRITC-dextran, MW 70000
    TRITC-dextran, MW 70000 (Tetramethyl rhodamine isothiocyanate glucan, MW 70000) is a fluorescent dye, with the molecular weight of 70 kD. TRITC-dextran, MW 70000 exhibits an excitation wavelength of 555 nm. TRITC-dextran, MW 70000 is utilized in drug delivery for the stability of TRITC over a wide pH range (i.e. pH 2–11) and resistance to photo-bleaching.
    TRITC-dextran, MW 70000
  • HY-D0824A
    CY7-SE triethylamine
    		≥98.0%
    CY7-SE triethylamine is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    CY7-SE triethylamine
  • HY-D1041
    ICG-OSu
    ICG-OSu (ICG NHS ester) is a near-infrared fluorescent agent ICG derivative. ICG-OSu is amine-reactive and has been widely used to design in vivo imaging probes.
    ICG-OSu
  • HY-15096
    MKT-077
    		98.05%
    MKT-077 (FJ-776), a highly water-soluble mitochondrial dye, has significant antitumor activity. MKT-077 exhibits low cytotoxicity, and inhibits broad-spectrum human cancer cell lines (colon cancer, breast cancer, pancreatic cancer). MKT-077 inhibits the growth of tumor in nude mice enograft tumor model. Ex/Em=488/543 nm.
    MKT-077
  • HY-D0309
    Rhodamine 6G
    		≥98.0%
    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.
    Rhodamine 6G
  • HY-D1498
    Mag-Fluo-4 AM
    Mag-Fluo-4 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Mag-Fluo-4 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.
    Mag-Fluo-4 AM
  • HY-101897
    Fura-2 AM
    		≥99.0%
    Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ (calcium ion) indicator.
    Fura-2 AM
  • HY-D1612
    BODIPY FL C5-Ceramide
    		99.65%
    The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY Fl C5-Ceramide is a Golgi-specific green fluorescent dye, which can visualise individual cells. Ex/Em= 505 nm/512 nm.
    BODIPY FL C5-Ceramide
  • HY-D1063
    IR-780
    IR780 is a prototypic NIR heptamethine cyanine agent, with a high molar extinction coefficient. The maximum excitation wavelength of IR780 was 777-780 nm, and the maximum emission wavelength was 798-823 nm. IR780 can be used in cancer PTT/PDT and imaging.
    IR-780
  • HY-D1416
    HMBR
    		98.43%
    HMBR is an analogue with an additional methyl group on the aromatic ring and is non-fluorescent. HMBR conjugated with Y-FAST emits yellow fluorescence under blue light excitation (Ex= 419 nm; Em= 525–539 nm). HMBR is non-toxic to zebrafish embryos. HMBR has high cell permeability.
    HMBR
  • HY-D1730A
    AF488 NHS ester TEA
    AF488 NHS ester TEA is an amine specific fluorescence probe (λem=525 nm=525 nm). AF488 NHS ester reacts with sulfhydryl groups and amines in aqueous and biological samples then change their chemical structure and fluorescence properties after derivatization. AF488 exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
    AF488 NHS ester TEA
  • HY-124409
    WSP-1
    		99.03%
    WSP-1 is a selective and rapid-reaction H2S specific fluorescent dye (Ex/Em=465/515 nm). WSP-1 reacts with H2S with the releasing of fluorophore.
    WSP-1
  • HY-D0155
    Zinpyr-1
    Zinpyr-1, a zinc-responsive fluorescent indicator that has found wide applicability for the detection of biological mobile zinc. Zinpyr-1 undergoes a shift in excitation from 515 to 507 nm with increasing zinc concentrations using an emission filter over 513-558 nm.
    Zinpyr-1
  • HY-N0573
    Umbelliferone
    		Activator 99.67%
    Umbelliferone (7-Hydroxycoumarin), a natural orally active product of the coumarin family, is a fluorescing compound which can be used as a sunscreen agent. Umbelliferone induces cell cycle arrest, apoptosis and DNA fragmentation in HepG2 cells. Umbelliferone exhibits significant anticancer effects. Umbelliferone attenuates the alteration characteristics of allergic airway inflammation. Umbelliferone displays the neuroprotective effects and cross the blood-brain barrier. Umbelliferone exhibits anti-inflammatory and antioxidant effects in chronic alcohol-fed rats.
    Umbelliferone
  • HY-137805
    Ferrozine
    		99.96%
    Ferrozine is a spectrophotometric reagent for iron ions, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm. Ferrozine-based colorimetric assays can quantify iron in cells
    Ferrozine
  • HY-D1808
    Vari Fluor 488-Streptavidin
    Vari Fluor 488-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=490 nm/515 nm.
    Vari Fluor 488-Streptavidin
  • HY-66021
    6-FAM
    		99.88%
    6-FAM (6-Carboxyfluorescein) is an isomer of carboxyfluorescein and is mainly used for sequencing and labeling of nucleic acids.
    6-FAM
  • HY-D0799
    Sulfo-NHS-LC-Biotin sodium
    		98.32%
    Sulfo-NHS-LC-Biotin sodium is an amine-reactive ester derivative of biotin and cannot pass the intact blood-retinal barrier. It can be used to assess vascular permeability of the brain and retinal vasculature.
    Sulfo-NHS-LC-Biotin sodium
Cat. No. Product Name / Synonyms Application Reactivity