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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D0970
    Diphenyl Blue
    Diphenyl Blue (Trypan Blue) is a cell active dye, the most commonly used dye for the identification of dead cells, of en used to test cell membrane integrity and cell viability. Diphenyl Blue staining is one of the methods for tissue and cell culture. When cells are deactivated or have incomplete cell membranes, Diphenyl Blue can stain them Blue. Normal living cells with intact cell membranes reject Diphenyl blue and do not stain them blue. However, macrophages are capable of phagocytosis of Diphenyl Blue, so it can be used as a living stain for macrophages.
    Diphenyl Blue
  • HY-D0286
    Fluorol Yellow 088
    		≥98.0%
    Fluorol Yellow 088 is a fluorescent dye that can be used as a biological material or an organic compound for life science research. Fluorol Yellow 088 is suitable for lipid staining in plant tissues, particularly for the fluorescence imaging of suberized cell walls and hydrophobic structures, with an excitation wavelength of 365 nm and an emission wavelength of ≥420 nm.
    Fluorol Yellow 088
  • HY-D0986
    TMA-DPH
    		99.86%
    TMA-DPH is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm). TMA-DPH is able to anchor on the cell surface and localize to different regions of the phospholipid bilayer. By analyzing the fluorescence polarization values of TMA-DPH in the plasma membrane and membrane substructures, the fluidity of the cell membrane can be determined.
    TMA-DPH
  • HY-D1041
    ICG-OSu
    ICG-OSu (ICG NHS ester) is a near-infrared fluorescent agent ICG derivative. ICG-OSu is amine-reactive and has been widely used to design in vivo imaging probes.
    ICG-OSu
  • HY-D1498
    Mag-Fluo-4 AM
    Mag-Fluo-4 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Mag-Fluo-4 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.
    Mag-Fluo-4 AM
  • HY-15096
    MKT-077
    		98.05%
    MKT-077 (FJ-776), a highly water-soluble mitochondrial dye, has significant antitumor activity. MKT-077 exhibits low cytotoxicity, and inhibits broad-spectrum human cancer cell lines (colon cancer, breast cancer, pancreatic cancer). MKT-077 inhibits the growth of tumor in nude mice enograft tumor model. Ex/Em=488/543 nm.
    MKT-077
  • HY-101897
    Fura-2 AM
    		≥99.0%
    Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ (calcium ion) indicator.
    Fura-2 AM
  • HY-D1416
    HMBR
    		98.43%
    HMBR is an analogue with an additional methyl group on the aromatic ring and is non-fluorescent. HMBR conjugated with Y-FAST emits yellow fluorescence under blue light excitation (Ex= 419 nm; Em= 525–539 nm). HMBR is non-toxic to zebrafish embryos. HMBR has high cell permeability.
    HMBR
  • HY-135985
    DCLK1-IN-1
    		99.35%
    DCLK1-IN-1 is a selective, oral bioavailability in vivo-compatible chemical probe of the doublecortin like kinase 1 (DCLK1 kinase) domain. DCLK1-IN-1 inhibits DCLK1 and DCLK2 kinases (IC50: DCLK1=9.5/57.2 nM and DCLK2=31/103 nM in binding and kinase assay, respectively). DCLK1-IN-1 shows low toxicity, and can investigate DCLK1 biology and establish its role in cancer, like DCLK1+ pancreatic ductal adenocarcinoma (PDAC).
    DCLK1-IN-1
  • HY-137805
    Ferrozine
    		99.96%
    Ferrozine is a spectrophotometric reagent for iron ions, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm. Ferrozine-based colorimetric assays can quantify iron in cells
    Ferrozine
  • HY-D0974
    Sulforhodamine B sodium salt
    		99.43%
    Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.
    Sulforhodamine B sodium salt
  • HY-D0903
    1,8-Diazafluoren-9-one
    		99.66%
    1,8-Diazafluoren-9-one (DFO) is a chemical that is used to find fingerprints on porous surfaces.
    1,8-Diazafluoren-9-one
  • HY-D1612
    BODIPY FL C5-Ceramide
    		99.65%
    The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY Fl C5-Ceramide is a Golgi-specific green fluorescent dye, which can visualise individual cells. Ex/Em= 505 nm/512 nm.
    BODIPY FL C5-Ceramide
  • HY-N0573
    Umbelliferone
    		Activator 99.67%
    Umbelliferone (7-Hydroxycoumarin), a natural orally active product of the coumarin family, is a fluorescing compound which can be used as a sunscreen agent. Umbelliferone induces cell cycle arrest, apoptosis and DNA fragmentation in HepG2 cells. Umbelliferone exhibits significant anticancer effects. Umbelliferone attenuates the alteration characteristics of allergic airway inflammation. Umbelliferone displays the neuroprotective effects and cross the blood-brain barrier. Umbelliferone exhibits anti-inflammatory and antioxidant effects in chronic alcohol-fed rats.
    Umbelliferone
  • HY-D1730A
    AF488 NHS ester TEA
    AF488 NHS ester TEA is an amine specific fluorescence probe (λem=525 nm=525 nm). AF488 NHS ester reacts with sulfhydryl groups and amines in aqueous and biological samples then change their chemical structure and fluorescence properties after derivatization. AF488 exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
    AF488 NHS ester TEA
  • HY-124409
    WSP-1
    		99.03%
    WSP-1 is a selective and rapid-reaction H2S specific fluorescent dye (Ex/Em=465/515 nm). WSP-1 reacts with H2S with the releasing of fluorophore.
    WSP-1
  • HY-138200
    Cy5 maleimide
    		99.43%
    Cy5 maleimide is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Cy5 maleimide
  • HY-158082C
    TRITC-dextran, MW 70000
    TRITC-dextran, MW 70000 (Tetramethyl rhodamine isothiocyanate glucan, MW 70000) is a fluorescent dye, with the molecular weight of 70 kD. TRITC-dextran, MW 70000 exhibits an excitation wavelength of 555 nm. TRITC-dextran, MW 70000 is utilized in drug delivery for the stability of TRITC over a wide pH range (i.e. pH 2–11) and resistance to photo-bleaching.
    TRITC-dextran, MW 70000
  • HY-B1025
    Digoxigenin
    		99.70%
    Digoxigenin (DIG) is a steroid. DIG is used for situ hybridization as a labeling molecule probe due to long shelf life and fast detection and high sensitivity of DIG-labeled riboprobes.
    Digoxigenin
  • HY-66021
    6-FAM
    		99.88%
    6-FAM (6-Carboxyfluorescein) is an isomer of carboxyfluorescein and is mainly used for sequencing and labeling of nucleic acids.
    6-FAM
Cat. No. Product Name / Synonyms Application Reactivity