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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1297
    ER-Tracker Green
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation.
    ER-Tracker Green
  • HY-101878
    Texas Red
    		99.72%
    Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.
    Texas Red
  • HY-D1421
    PKH 67
    		≥99.0%
    PKH67 is a fluorescent cell binding dye with green fluorescence. PKH67 can stain the cell membrane and the Ex/Em is 490/502 nm. PKH67 is often used in combination with the non-specific red fluorescent dye PKH26 (Ex/Em=551/567 nm) to label cells, detect cell proliferation in vitro, and trace cells in vitro and in vivo.
    PKH 67
  • HY-D1434
    FM1-43
    		98.67%
    FM1-43 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers.
    FM1-43
  • HY-119287
    TSQ
    		99.50%
    TSQ is a cytosolic zinc ion fluorescence probe that is membrane permeable and can be used for intracellular imaging of zinc proteins (λmax ~470 nm). TSQ can combine with Zn2+ in the presence of Ca2+ and Mg2+ to produce blue fluorescence (Ex/Em = 334/495 nm).
    TSQ
  • HY-138226
    BODIPY 558/568 C12
    		98.42%
    BODIPY 558/568 C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 558/568 nm.
    BODIPY 558/568 C12
  • HY-D1168
    Oil Red O
    Oil Red O is a fat-soluble diazol dye, with a maximum absorption at 518 nm. Oil Red O stains neutral lipids and cholesteryl esters but not biological membranes. Oil Red O can be used for detecting and quantifying hepatic steatosis in mouse liver biopsies. Oil Red O staining efficiently helps to visualize the radical changes that occur in tissues as metabolic disease occurs and progresses.
    Oil Red O
  • HY-D0717
    DAF-FM DA
    		98.32%
    DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm).
    DAF-FM DA
  • HY-D0993
    Acridine Orange 10-Nonyl Bromide
    		99.50%
    Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).
    Acridine Orange 10-Nonyl Bromide
  • HY-129420
    WSP-5
    		99.45%
    WSP-5 is a fluorescent probe for fast detection of H2S in biological samples and cells. WSP-5 selectively and rapidly reacts with H2S and exhibits fluorescence through a reaction-based fluorescent turn-on strategy.
    WSP-5
  • HY-W074143
    Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride
    		99.78%
    Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride ([Ru(dpp)3]2+) is an electrochemiluminescent (ECL) probe that can be used to modify the electrode surface to detect sulfate (S2O82-) and oxalate. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride can generate excited state species based on electrochemical reactions and release photons through irreversible redox reactions. When used as a cathode to detect persulfate, it can avoid the interference of hydrogen evolution. At the same time, the anode shows a wide linear range for oxalate. Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride can be used to construct a regenerable ECL sensor.
    Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride
  • HY-117695
    AQC
    AQC (6-Aminoquinolyl-N-hydroxysccinimidyl carbamate) is a reagent used for amino acid or protein sequence analysis by HPLC with fluorescence detection. AQC reacts with primary and secondary amino acids to yield fluorescent derivates, allowing amino acid detection at under-picomolar levels.
    AQC
  • HY-D0150
    Thiazole Orange
    		99.61%
    Thiazole Orange is an asymmetric anthocyanin dye that can be coupled with oligonucleotides (ONs) to prepare fluorescent hybridization probes. Thiazole Orange has been widely used in biomolecular detection and staining of DNA/ RNA in gels and can be used for reticulocyte analysis. Thiazole orange generates a significant fluorescence enhancement and high quantum yield when it binds with nucleic acids, especially RNA. Thiazole orange can permeate living cell membranes. Thiazole orange can use UV light for detection, but can also be detected with blue light. The excitation and emission of Thiazole orange are λex = 510 nm (488 nm and 470 nm also show strong excitation) and λem = 527 nm, respectively.
    Thiazole Orange
  • HY-D0098
    Fluorescein-5-maleimide
    		99.48%
    Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm.
    Fluorescein-5-maleimide
  • HY-126793
    2′,7′-Dichlorofluorescein diacetate
    		98.95%
    2′,7′-Dichlorofluorescein diacetate (DCFH2-DA) is a cell-permeable fluorescent probe. 2′,7′-Dichlorofluorescein diacetate can be used to detect the generation of reactive oxygen intermediates and for assessing the overall oxidative stress in toxicological phenomenon.
    2′,7′-Dichlorofluorescein diacetate
  • HY-111496
    Sulfo-NHS-SS-Biotin sodium
    Sulfo-NHS-SS-biotin sodium is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin sodium can be used for the labeling and purifying of cell-surface protein.
    Sulfo-NHS-SS-Biotin sodium
  • HY-N7131
    Coumarin 6
    		99.93%
    Coumarin 6, a fluorescent dye, is used as a fluorescent probe in a microparticle drug delivery system to conduct in vivo tracking, cell uptake, and transport mechanism studies of drug delivery systems (λexc=450 nm, λem=505 nm).
    Coumarin 6
  • HY-D0257
    Tartrazine
    		99.85%
    Tartrazine (Acid Yellow 23; FD&C Yellow No. 5) is an orally active azo acid dye, orange-yellow powder, soluble in water and turns yellow. Tartrazine is mainly used as a synthetic lemon yellow azo dye for food coloring. Tartrazine is the most stable colorant.Tartrazine induces mitochondria-mediated Apoptosis. Tartrazine can cause neurodevelopmental toxicity, cytotoxicity, and genotoxicity.
    Tartrazine
  • HY-15558
    Hoechst 33258
    		99.93%
    Hoechst 33258 is a blue to blue-green fluorescent live cell dye that can label DNA. Hoechst 33258 can specifically bind to the minor groove of DNA (and tends to bind to A/T-rich DNA), resulting in a significant increase in fluorescence intensity. Hoechst 33258 can cross the cell membrane and cause changes in DNA structure, such as G2/M phase arrest. Hoechst 33258 can bind to live or fixed cells, and the fluorescence intensity increases with increasing solution pH. As a DNA-specific probe, Hoechst 33258 can be used to detect DNA content, analyze cell cycle, etc. The excitation wavelength of Hoechst 33258 is 350-365 nm, and the emission wavelength is 460-490 nm.
    Hoechst 33258
  • HY-133870
    Itaconate-alkyne
    		98.01%
    Itaconate-alkyne (ITalk) is a specific bioorthogonal probe for quantitative and site-specific chemoproteomic profiling of Itaconation in living cells. Itaconate-alkyne, a functional analogue of Itaconate, exhibits comparable antiinflammatory effect with Itaconate and enables the labeling of bona fide targets of Itaconate. Itaconate-alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    Itaconate-alkyne
Cat. No. Product Name / Synonyms Application Reactivity