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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-B0324A
    Crystal Violet
    Crystal Violet, also known as Gentian violet, methyl violet 10B, is a triphenyl-methane, an alkaline dye that binds to DNA in the nucleus of a cell, staining it a deep purple. It is often used for Gram staining to classify bacteria, or for cell or histological staining[1].
    Crystal Violet
  • HY-D0822
    CY3
    		99.87%
    Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
    CY3
  • HY-D0040
    Calcein
    Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems.
    Calcein
  • HY-151857
    TAMRA azide, 5-isomer
    		98.94%
    TAMRA azide, 5-isomer is a click chemical containing azide groups that can be used as a linker for the orange dye TAMRA. The azide group of TAMRA Azide, 5-isomer enables copper-catalyzed click chemical reactions with alkynes, DBCO, and BCN. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    TAMRA azide, 5-isomer
  • HY-101899
    Monochlorobimane
    		99.86%
    Monochlorobimane (Chlorobimane) is a fluorescent dye (λex=380 nm, λem=470 nm) to measure glutathione (GSH) in cellular assays.
    Monochlorobimane
  • HY-111382
    Diphenylterazine
    		98.93%
    Diphenylterazine (DTZ) is a bioluminescence agent. Diphenylterazine alone yielded very little background, leading to excellent signal-to-background ratios.
    Diphenylterazine
  • HY-Y0016
    Rhodamine B
    		≥98.0%
    Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.
    Rhodamine B
  • HY-D0831
    WST-8
    		99.69%
    WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
    WST-8
  • HY-W019823
    4-MUNANA
    		98.08%
    4-MUNANA is a substrate of influenza virus neuraminidase (NA) with high selectivity and irreversible reaction. In the enzymatic reaction, 4-MUNANA is hydrolyzed by NA to generate fluorescent 4-methylumbelliferone (4-MU). By detecting the fluorescence intensity of 4-MU, quantitative analysis of NA activity can be achieved. 4-MUNANA can be used in influenza-related research, such as screening NA inhibitors, developing new anti-influenza drugs, and studying the infection mechanism of influenza viruses.
    4-MUNANA
  • HY-112641A
    AkaLumine hydrochloride
    		99.92%
    AkaLumine hydrochloride is a D-luciferin (HY-12591A) analogue with a Km of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine hydrochloride emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine hydrochloride has high tissue-penetration and increases detection sensitivity from deep-tissue targets.
    AkaLumine hydrochloride
  • HY-15902
    ABTS diammonium salt
    		99.86%
    ABTS diammonium salt (AzBTS-(NH4)2) is a substrate for horseradish peroxidase (HRP) conjugate. ABTS diammonium salt can be used to evaluate antioxidant capacity.
    ABTS diammonium salt
  • HY-126561
    Green CMFDA
    		99.82%
    Green CMFDA is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.
    Green CMFDA
  • HY-15922
    Luminol
    		99.95%
    Luminol is a chemical that exhibits chemiluminescence with pKa values of 6.74 and 15.1. Luminol exhibits chemiluminescence (CL) at 425 nm λmax. Luminol is commonly used in forensics as a diagnostic tool for the detection of blood stains.
    Luminol
  • HY-141898
    Biotin-4-aminophenol
    		99.45%
    Biotin-4-aminophenol is a biotin-phenol analog. Biotin-4-aminophenol generates free radicals and conjugates to tyrosine residues in proteins more efficiently and selectively than the previously reported BP1.
    Biotin-4-aminophenol
  • HY-128692
    Lucifer Yellow CH dilithium salt
    		99.67%
    Lucifer Yellow CH dilithium salt is a high-intensity fluorescent probe containing free hydrazyl groups. Lucifer Yellow CH can react with fatty aldehydes at room temperature. Lucifer Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Lucifer yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively.
    Lucifer Yellow CH dilithium salt
  • HY-D2270
    Halo tag TMR
    Halo tag TMR is a red fluorescent dye composed of Halo tag ligand molecules and TMR. Halo tag can quickly and stably covalently bind to Halo protein with high specificity and affinity.
    Halo tag TMR
  • HY-101878
    Texas Red
    		99.72%
    Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.
    Texas Red
  • HY-D1297
    ER-Tracker Green
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation.
    ER-Tracker Green
  • HY-D0208
    Fluorescein sodium
    Fluorescein (Uranine) sodium is widely used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer. Fluorescein (Uranine) sodium is a representative green fluorophore that has been widely used as a scaffold of practically useful green fluorescent probes.
    Fluorescein sodium
  • HY-U00451
    ATP-Red 1
    		≥98.0%
    ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).
    ATP-Red 1
Cat. No. Product Name / Synonyms Application Reactivity