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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2205
    Cy7 tyramide
    Cy7 tyramide, a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.
    Cy7 tyramide
  • HY-NP043
    Avidin-Cy3
    Avidin-Cy3 is Cy3-labeled Avidin. Avidin has excellent affinity with biotin and is often used in combination with biotin for immunoassays to detect the location of antigens in tissues.
    Avidin-Cy3
  • HY-D1550
    10-(4-(Bis(2-acetoxyethyl)amino)phenyl)-BODIPY 505/515
    		98.78%
    10-(4-(Bis(2-acetoxyethyl)amino)phenyl)-BODIPY 505/515 is a fluorescent dye for Cu2+ ion.
    10-(4-(Bis(2-acetoxyethyl)amino)phenyl)-BODIPY 505/515
  • HY-D1880
    Sulfo-Cy5 hydrazide
    Sulfo-Cy5 hydrazide is a fluorescent dye containing a hydrazide functionality (Ex/Em=646/662 nm).
    Sulfo-Cy5 hydrazide
  • HY-W250928C
    Biotin-PEG-azide (MW 2000)
    		98.69%
    Biotin-PEG-azide (MW 2000) is a biotin labeled PEG derivative. Biotin is an enzyme co-factor, can be used for labeling protein; PEG is a hydrophilic and water-soluble polymer with low toxicity; azide, is a moderately good leaving group, can react with alkyne by Cu-catalyzation, which improve the efficiency of biotin binding targets. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    Biotin-PEG-azide (MW 2000)
  • HY-D1075
    1,1',3,3,3',3'-Hexamethylindodicarbocyanine iodide
    		99.73%
    1,1',3,3,3',3'-Hexamethylindodicarbocyanine iodide is a carbocyanine dye that stains mitochondria of live cells.
    1,1',3,3,3',3'-Hexamethylindodicarbocyanine iodide
  • HY-123495
    CRANAD-28
    		98.03%
    CRANAD-28 is a robust fluorescent compound for visualization of amyloid beta plaques.
    CRANAD-28
  • HY-D0801
    Calmagite
    		98.0%
    Calmagite is a chromomeric reagent which can be used to detect calcium and magnesium in various samples.
    Calmagite
  • HY-W713926
    1-Pyrenedecanoic acid
    		Chemical 99.34%
    1-Pyrenedecanoic acid is a biochemical reagent, and can be used for membrane fluidity assessment.
    1-Pyrenedecanoic acid
  • HY-133876
    4-Di-16-ASP
    		99.76%
    4-Di-16-ASP is a green fluorescent membrane dye. 4-Di-16-ASP is a lipophilic aminostyryl probe with a broad emission spectrum (can be detected with green, orange or even red filters). It is commonly used for neuronal membrane tracing (it diffuses faster than DiO).
    4-Di-16-ASP
  • HY-W800705
    Tamra azide
    		98.97%
    Tamra azide is a red fluorescent probe. The azide group of Tamra azide can react with alkynes via copper-catalyzed click chemistry.
    Tamra azide
  • HY-168375
    BODIPY-Palmitate
    BODIPY-Palmitate is a fluorescently labeled palmitic acid (HY-N0830), with excitation/emission maxima at 488/508 nm. BODIPY-Palmitate can be used to detect fatty acid uptake and metabolism.
    BODIPY-Palmitate
  • HY-W127832
    Ru(dpp)3(PF6)2
    		98.20%
    Ru(dpp)3(PF6)2 is a complex containing metallic ruthenium (Ru) and is a chemiluminescent oxygen probe. [Ru(dpp)3]2+ is a luminescent substance that can undergo metal-to-ligand charge transfer (MLCT), and fluorescence will appear during the transfer of Ru to the ligand. At the same time, the fluorescence of Ru(dpp)3(PF6)2 is effectively quenched by molecular oxygen, so it is widely used in biosensors, environmental monitoring, and materials science. The property of Ru(dpp)3(PF6)2, which oxidizes Ru2+ to Ru3+, has also been used to design and construct solid-state light-emitting electrochemical cells.
    Ru(dpp)3(PF6)2
  • HY-D0830
    Calcein (tetraethyl ester)
    		99.52%
    Calcein tetraethyl ester is a fluorescent dye, is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells.
    Calcein (tetraethyl ester)
  • HY-D2339
    H-FluNox
    		99.53%
    H-FluNox is a selective fluorescent probe that senses labile heme using biomimetic N-oxide deoxygenation to trigger fluorescence. H-FluNox is more than 100-fold selective for labile heme over Fe(II), enabling the discrimination of labile heme from labile Fe(II) pools in living cells. H-FluNox is sensitive enough to detect the subcellular labile heme. (λex=490 nm, λem=535 nm)
    H-FluNox
  • HY-160870
    Firefly luciferase-IN-3
    Firefly luciferase-IN-3 (CID: 17087944) is an inhibitor of ATP-dependent luciferase (Firefly luciferase) and has nM-level inhibitory activity against NanoLuc (pIC50=7.5).
    Firefly luciferase-IN-3
  • HY-D1050
    DNP-X, SE
    		98.47%
    DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester), the DNP-X acid modified by succinimidyl ester, is an amine-reactive building block for developing a probe, which can be recognized by anti-DNP antibodies. DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester) is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.
    DNP-X, SE
  • HY-D1444
    Propidium monoazide
    		99.30%
    Propidium monoazide is a photoreactive DNA-binding dye that preferentially binds to dsDNA. Propidium monoazide (PMA) prevents DNA from dead bacteria from being amplified during the PCR. PMA-PCR enhanced both the specificity and the sensitivity of PCR. Propidium monoazide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Propidium monoazide
  • HY-101937C
    (±)-ANAP hydrochloride
    (±)-ANAP hydrochloride is the unnatural amino acid analog of prodan, acts as a fluorescent probes, and enhances environmental sensitivity.
    (±)-ANAP hydrochloride
  • HY-126220A
    KMG-301AM TFA
    KMG-301AM TFA is the acetoxy methyl esterified form of KMG-301. KMG-301AM TFA successfully accumulates in mitochondria and then it is hydrolyzed to KMG-301. KMG-301 is an Mg2+-selective fluorescent probe functional in mitochondria in intact cells. Since the mitochondrial membrane is impermeable to KMG-301, it is not released upon depolarization of the mitochondrial membrane potential. KMG-301 can indicate changes in mitochondrial Mg2+ concentration and shows Mg2+ transport across the mitochondrial membrane in the early phases of a cellular model.
    KMG-301AM TFA
Cat. No. Product Name / Synonyms Application Reactivity