2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-P3949
    Fluorescent Substrate for Glu-Specific Proteases
    		98.16%
    Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate.
    Fluorescent Substrate for Glu-Specific Proteases
  • HY-125815
    Reactive Blue 4
    Reactive Blue 4 is an anthraquinone dye used extensively in the textile industry. Reactive Blue 4 is a single colorimetric chemosensor for sequential determination of multiple analytes with different optical responses in aqueous media.
    Reactive Blue 4
  • HY-D2173
    AF488 azide
    		≥99.0%
    AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. It contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups. AF488 exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
    AF488 azide
  • HY-D1818
    Vari Fluor 680-Phalloidin
    Vari Fluor 680-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence when labeled at 680/700 nm.
    Vari Fluor 680-Phalloidin
  • HY-D2205
    Cy7 tyramide
    Cy7 tyramide, a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.
    Cy7 tyramide
  • HY-D2083
    BODIPY 540 (purity 99%)
    		99.32%
    BODIPY 540 (purity>98%) is a BODIPY dye.BODIPY dye is a small molecule dye with strong UV absorption ability.Its fluorescence peak is relatively sharp, its quantum yield is high, and it is relatively insensitive to the polarity and pH value of the environment.BODIPY 540 (purity>98%) has a purity higher than 98% and is suitable for cell experiments.
    BODIPY 540 (purity 99%)
  • HY-P0286F1A
    FITC-OVA (323-339) TFA
    FITC-OVA (323-339) TFA is a biological active peptide. (FITC labeled HY-P0286)
    FITC-OVA (323-339) TFA
  • HY-D2161A
    AF 594 azide triethylamine
    AF 594 azide (triethylamine) is an azide derivative of the red fluorescent dye AF 594, which has high fluorescence quantum yield and high photostability (maximum absorption wavelength of 586 nm, maximum emission wavelength of 613 nm). AF 594 azide (triethylamine) forms stable adducts by reaction of the azide group with alkynyl derivatives (terminal alkynes and cyclooctyne).
    AF 594 azide triethylamine
  • HY-W010947
    4-Methylumbelliferyl palmitate
    		99.23%
    4-Methylumbelliferyl palmitate is an excellent fluorophore for measuring acid lipase in human leukocytes. Acidity and solvent have important influence on its fluorescence. 4-Methylumbelliferyl palmitate exists mainly as neutral molecular form which can be produced strong fluorescence at 445 nm in near neutral aqueous solutions, and exist mainly as anion form which can be produced stronger fluorescence at 445 nm in weak alkaline solutions.
    4-Methylumbelliferyl palmitate
  • HY-139337
    SMCy5.5
    SMCy5.5 is an SMCy dye used for lipid droplet labeling with a very high two-photon absorption cross-section. SMCy, unlike the well-known lipid droplet marker Nile Red, has narrow absorption and emission bands in visible light, allowing for multicolor imaging. SMCy is shown to be compatible with fixation, producing high-quality 3D images of lipid droplets in cells and tissues. The high brightness of SMCy5.5 enables efficient tracking and imaging of lipid droplet exchange between adipocytes.
    SMCy5.5
  • HY-D0220A
    Toluidine Blue (purity 36%)
    		99.34%
    Toluidine Blue (Toluidine Blue O) purity 36% is an alkaline quinonimine dye (vivo dye) with high affinity for acidic tissue components, staining nuclei blue and polysaccharides purple. Toluidine Blue purity 36% shows heterostaining properties for mast cells, mucins and chondrocytes. Toluidine Blue purity 36% can stain different components of plant tissues and cells in different colours. Toluidine Blue purity 36% is also used as a diagnostic aid to identify malignant lesions, such as cancer.
    Toluidine Blue (purity 36%)
  • HY-145127
    ETZ
    ETZ (C3-CA-DTZ) is a promising luciferase substrate (prosubstrate) activatable in vivo by nonspecific esterase to enhance the brain delivery of the luciferin.
    ETZ
  • HY-D1156A
    HKSOX-1m (5/6-mixture) hydrobromide
    		99.55%
    HKSOX-1m (5/6-mixture) hydrobromide is a O2 fluorescent probe for mitochondria-targeting (Ex/Em=509/534nm; green), exhibiting excellent selectivity and sensitivity toward O2 over a broad range of pH, strong oxidants, and abundant reductants found in cells.
    HKSOX-1m (5/6-mixture) hydrobromide
  • HY-155640
    5-FAM maleimide
    		98.54%
    5-FAM maleimide is a 5-FAM (HY-66022) derivative. 5-FAM maleimide can be used to label proteins.
    5-FAM maleimide
  • HY-123495
    CRANAD-28
    		98.03%
    CRANAD-28 is a robust fluorescent compound for visualization of amyloid beta plaques.
    CRANAD-28
  • HY-D0075
    DHPDS disodium salt
    DHPDS disodium salt is a pH-sensitive fluorescent dye. DHPDS disodium salt is used to measure intracellular pH (pHi) from the surface fluorescence of the isolated perfused rat liver.
    DHPDS disodium salt
  • HY-D0931
    Sudan III
    		98.21%
    Sudan III is a hydrophobic bisazo dye.
    Sudan III
  • HY-D0025
    7-Amino-4-methylcoumarin-3-acetic acid
    		99.85%
    7-Amino-4-methylcoumarin-3-acetic acid is a fluorescent protein labelling agent. 7-Amino-4-methylcoumarin-3-acetic acid emits in the blue region (440-460 nm) on activation with UV light (350 nm).
    7-Amino-4-methylcoumarin-3-acetic acid
  • HY-15914
    DHBS
    		99.79%
    DHBS (3, 5-dichloro-2-hydroxybenzenesulfonate) is used in conjunction with 4-aminoantipyrine (4-AAP) (HY-W100990) and hydrogen peroxide (H2O2) for chromogenic quantitation of peroxidase in coupled enzymatic reactions. Component of Trinder reagent for use with peroxidase to measure generation of hydrogen peroxide in automated systems.
    DHBS
  • HY-D1736
    BODIPY FL-C16
    		98.02%
    BODIPY FL-C16 is a fluorescent probe that combines BODIPY FL dye and a sixteen-carbon fatty acid chain, and it can be used for lipid metabolism-related research.
    BODIPY FL-C16
Cat. No. Product Name / Synonyms Application Reactivity