2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-103466
    FM4-64
    		99.94%
    FM4-64 is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM4-64 is widely used in endocytic and exospic membrane structure markers.
    FM4-64
  • HY-101879
    Acridine Orange hydrochloride
    		99.86%
    Acridine Orange hydrochloride is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm).
    Acridine Orange hydrochloride
  • HY-D1462
    CellTracker Blue CMAC
    		98.84%
    CellTracker Blue CMAC is a non-fluorescent cell membrane permeable dye. The chloromethyl groups of CellTracker Blue CMAC are enzymatically cleaved by intracellular glutathione (GSH) to generate a fluorescent product (blue fluorescence, Ex/Em: 360/460 nm). CellTracker Blue CMAC is suitable for long-term cell tracking (up to 72 hours) and cell proliferation studies, and can also quantify GSH levels.
    CellTracker Blue CMAC
  • HY-D0819
    CY5-SE
    		98.10%
    Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.
    CY5-SE
  • HY-13594
    Chlorin e6
    		99.51%
    Chlorin e6 is a photosensitizer and has strong absorption peaks at wavelength of 402 and 662 nm, as well as exhibiting intense fluorescence at 668 nm. Chlorin e6 has antimicrobial efficacy and anticancer activity. Chlorin e6 induces cell apoptosis via caspase-3 activation and can be used for the research of cancer.
    Chlorin e6
  • HY-126839
    C12FDG
    		99.84%
    C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di(β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm).
    C12FDG
  • HY-D0085
    DiSC3(5)
    		99.37%
    DiSC3(5) is a fluorescent probe commonly used as a tracer dye to evaluate mitochondrial membrane potential. The excitation/emission wavelength of DiSC3(5) is up to 622/670 nm. DiSC3(5) can inhibit the respiratory system associated with mitochondrial NAD, and the IC50 value is 8 μM. DiSC3(5) in the presence of Na+/K+-ATPase inhibitor ouabain 2 can induce membrane hyperpolarization of Ehrlich ascites tumor cells.
    DiSC3(5)
  • HY-101892
    DiBAC4(3)
    		99.91%
    DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm). DiBAC4(3) can be used to measure cell membrane potential.
    DiBAC4(3)
  • HY-D0802
    Biotin NHS
    		99.85%
    Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.
    Biotin NHS
  • HY-101894
    Dihydrorhodamine 123
    		99.83%
    Dihydrorhodamine 123 (DHR 123) is a non-fluorescent reactive oxygen species (ROS) indicator. Dihydrorhodamine 123 is oxidized to fluorescent Rhodamine 123 (HY-D0816) within cells in the presence of reactive oxygen species and it localizes in mitochondria (Ex/Em = 515/536 nm).
    Dihydrorhodamine 123
  • HY-D0972
    Thioflavine S
    Thioflavine S is a fluorescent histochemical marker of dense core senile plaques. Thioflavine S can be used for Alzheimer's research.
    Thioflavine S
  • HY-111391
    Resazurin sodium
    		≥98.0%
    Resazurin sodium (Diazoresorcinol sodium) is a non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin sodium is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin sodium can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal).
    Resazurin sodium
  • HY-D0825
    CY7
    		99.08%
    CY7 is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    CY7
  • HY-128868D
    FITC-Dextran (MW 40000)
    FITC-Dextran (MW 40000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 40000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 40000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.
    FITC-Dextran (MW 40000)
  • HY-D1300
    LysoTracker Red
    		98.38%
    LysoTracker Red is a Red fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 577/590 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and gather on spherical organelles. It is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes.
    LysoTracker Red
  • HY-D0816
    Rhodamine 123
    		99.55%
    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.
    Rhodamine 123
  • HY-P1002
    Suc-Leu-Leu-Val-Tyr-AMC
    		99.66%
    Suc-Leu-Leu-Val-Tyr-AMC is a membrane-permeable calpain-specific fluorogenic substrate (Ex/Em = 390/480 nm).
    Suc-Leu-Leu-Val-Tyr-AMC
  • HY-D2270
    Halo tag TMR
    Halo tag TMR is a red fluorescent dye composed of Halo tag ligand molecules and TMR (TAMRA). Halo tag can quickly and stably covalently bind to Halo protein with high specificity and affinity (Ex/Em = 550/576 nm).
    Halo tag TMR
  • HY-W011664
    1,3-Diphenylisobenzofuran
    		98.74%
    1,3-Diphenylisobenzofuran (DPBF) has been developed as a selective probe for the detection and quantitative determination of hydrogen peroxide in samples containing different reactive nitrogen and oxygen species (RNOS). DPBF is a fluorescent probe which, for almost 20 years, was believed to react in a highly specific manner toward some reactive oxygen species such as singlet oxygen and hydroxy, alkyloxy or alkylperoxy radicals.
    1,3-Diphenylisobenzofuran
  • HY-15934
    X-GAL
    		99.88%
    X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity.
    X-GAL
Cat. No. Product Name / Synonyms Application Reactivity