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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2096A
    Alexa fluor 647 NHS ester TEA
    Alexa fluor 647 NHS ester TEA can be used to label Alexa fluor 647 to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
    Alexa fluor 647 NHS ester TEA
  • HY-W088068
    Wright's stain
    Wright's stain is a composite cell stain that mainly binds to intracellular nucleic acids, proteins and other components through thiazine dyes (such as methylene blue) and eosin. Wright's stain is pH-dependent (optimal pH 6.4-6.7) and achieves cell morphology resolution by differentially staining the cytoplasm and nucleus. Under alkaline conditions, thiazine dyes bind to nucleic acids to form purple, and acidic eosin binds to cytoplasmic proteins to form red, which can form contrasting cell morphological features. Wright's stain can clearly display the fine structures of blood cells and bone marrow cells (such as nuclear chromatin and granules) and quickly evaluate cell morphological abnormalities.
    Wright's stain
  • HY-D0941
    5-Carboxytetramethylrhodamine
    		99.13%
    5-Carboxytetramethylrhodamine can be used as a fluorescent probe of nucleic acids and proteins. 5-Carboxytetramethylrhodamine displays excitation maxima of 558 nm and an emission maximum of 586 nm.
    5-Carboxytetramethylrhodamine
  • HY-D1445
    LysoSensor PDMPO
    		98.77%
    PDMPO, a lysosome pH indicator, is an excellent fluorescent acidotropic reagent for fluorescence imaging. PDMPO is a potent tool with which to study acidic organelles of live cells. PDMPO exhibits pH-dependent dual-excitation and dual-emission spectral peaks. PDMPO produces a blue fluorescence in weakly acidic organelles and shifts to yellow in more acidic lysosomes (Abs=329 nm; Em=440 nm).
    LysoSensor PDMPO
  • HY-153231
    eGFP mRNA-LNP
    eGFP mRNA-LNP is a lipid nanoparticle (LNP) containing eGFP mRNA. eGFP mRNA-LNP carries Enhanced Green Fluorescent Protein (eGFP), which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry (excitation wavelength = 488 nm; emission wavelength = 535/40 bandpass). eGFP mRNA-LNP can be used for RNA delivery, translation efficiency and cell viability.
    eGFP mRNA-LNP
  • HY-D0166
    Neutral Red
    		98.14%
    Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids. Neutral Red stains lysosomes red.
    Neutral Red
  • HY-158082B
    TRITC-dextran, MW 40000
    TRITC-dextran, MW 40000 (Tetramethyl rhodamine isothiocyanate glucan, MW 40000) is a fluorescent dye, with the molecular weight of 40 kD. TRITC-dextran MW 40000 exhibits an excitation wavelength of 555 nm. TRITC-dextran MW 40000 is vessel penetrate, which could label blood plasma to visualize the vasculature. TRITC-dextran MW 40000 is utilized in drug delivery for the stability of TRITC over a wide pH range (i.e. pH 2–11) and resistance to photo-bleaching.
    TRITC-dextran, MW 40000
  • HY-D1568
    Sulfo-Cy7.5 NHS ester
    Sulfo-Cy7.5 NHS ester is a near infrared water soluble hydrophilic dye, also is an NHS ester for the modification of amine groups. Sulfo-Cy7.5 NHS ester contains a trimeth ylene bridge and has a linker arm for its attachment to proteins, peptides, and other molecules. Sulfo-Cy7.5 NHS ester can be used for the research of NIR imaging applications.
    Sulfo-Cy7.5 NHS ester
  • HY-D0076
    4,5-Diaminofluorescein
    		99.34%
    4,5-Diaminofluorescein is a fluorescent detector for nitric oxide (NO) in cells and tissues.
    4,5-Diaminofluorescein
  • HY-D1872
    800CW NHS ester
    800CW NHS ester is a near infrared probe that can be used for labeling lysine residues (λmax=776 nm).
    800CW NHS ester
  • HY-131027
    Janelia Fluor® 646, Azide
    		99.87%
    Janelia Fluor 646, Azide (JF646, Azide) is a red fluorogenic fluorescent dye containing a click chemistry group Azide. Janelia Fluor 646, Azide can be used for live-cell imaging experiments. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor? 646, Azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Janelia Fluor® 646, Azide
  • HY-128448
    Carmoisine
    		≥98.0%
    Carmoisine (Azorubine) is an azo dye that can be used as a food additive.
    Carmoisine
  • HY-114616
    PBB3
    PBB3 is a tracer of tau PET. PBB3 can be used to detect levels of tau protein in Alzheimer's disease and non-Alzheimer's disease.
    PBB3
  • HY-D0053
    6-ROX
    		99.00%
    6-ROX is a selective fluorescent probe and potential inhibitor of COX-2. 6-ROX binds to the active site of COX-2 and inhibits its conversion of arachidonic acid into prostaglandins. 6-ROX is often used in the field of optical imaging related to tumors and inflammation, and helps detect diseased tissues with high expression of COX-2.
    6-ROX
  • HY-W588722
    ICG-DBCO
    ICG-DBCO is a near-infrared fluorescent dye that is widely used in cell dyes, biological dyes, biomolecules and particle fluorescent labeling.Ex/Em = 789/813 nm
    ICG-DBCO
  • HY-D0215
    Safranin
    Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. Safranin can readily intercalate into biological macromolecules, including DNA and proteins. Safranin has antibacterial effects against gram-positive bacteria (S. aureus). Safranin can be used as a redox indicator in the determination of metal ion concentration.
    Safranin
  • HY-108715
    Real Thiol
    		98.37%
    Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.
    Real Thiol
  • HY-D1030
    Fluorescein Biotin
    Fluorescein Biotin is used as an alternative to radioactive biotin for detecting and quantitating biotin-binding sites by either fluorescence or absorbance; the the fluorescence or absorbance of Fluorescein Biotin is quenched, upon binding to avidin or streptavidin.
    Fluorescein Biotin
  • HY-135414B
    Cyanine5 NHS ester iodide
    		99.95%
    Cyanine5 NHS ester iodide is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides.
    Cyanine5 NHS ester iodide
  • HY-D0342
    Disperse Red 1
    Disperse Red 1, an azobenzene derivative, is an azo textile dye extensively used for dyeing polyester fabrics in textile industry.
    Disperse Red 1
Cat. No. Product Name / Synonyms Application Reactivity