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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-124171
    Zinquin ethyl ester
    		≥99.0%
    Zinquin ethyl ester is a fluorescent derivative of Zinquin and is a fluorescent probe of cytosolic zinc ion. Zinquin ethyl ester is able to penetrate cell membranes and is lipophilic and zinc-sensitive. Zinquin ethyl ester can combine with Zn2+ in the cell to produce blue fluorescence.
    Zinquin ethyl ester
  • HY-D1449
    IR-1061
    IR-1061 is a near infrared fluorescent organic dye for in vivo bimodal imaging.
    IR-1061
  • HY-D0819A
    CY5-SE triethylamine salt
    		≥98.0%
    Cy5-SE (Cy5 NHS Ester) triethylamine salt is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Cy5-SE triethylamine salt is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides Ex=649 nm; Em=670 nm).
    CY5-SE triethylamine salt
  • HY-137592
    Nicotinamide 1,N6-ethenoadenine dinucleotide
    		≥99.0%
    Nicotinamide 1,N6-ethenoadenine dinucleotide (ε-NAD) , a fluorescent analogue of NAD, is able to serve as a substrate for the bacterial toxincatalyzed G-ADP ribosylation of signal-transducing G-proteins. Nicotinamide 1,N6-ethenoadenine dinucleotide can be used as a fluorescent substrate for the studies of the ADP ribosylation reaction.
    Nicotinamide 1,N6-ethenoadenine dinucleotide
  • HY-136626
    Z-Ala-Ala-Asn-AMC
    		99.47%
    Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC.
    Z-Ala-Ala-Asn-AMC
  • HY-110250
    DFHBI
    		99.39%
    DFHBI is a small molecule that resembles the chromophore of green fluorescent protein (GFP). Spinach and DFHBI are essentially nonfluorescent when unbound, whereas the Spinach-DFHBI complex is brightly fluorescent both in vitro and in living cells.
    DFHBI
  • HY-D1837
    Vari Fluor 488 TSA（200×）
    Vari Fluor 488 TSA（200×） (VF 488 Tyramide) is a fluorescent probe belonging to the Vari Fluor TSA series. Vari Fluor TSA series fluorescent probes can be used for high-density in situ labeling of target antigens using horseradish peroxidase (HRP) assays, which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 488 TSA（200×）
  • HY-N1389
    Carmine
    Carmine (Carmine red), a natural red dye extracted from the dried females of the insect Dactylopius coccus var. Costa (cochineal). Carmine is a widely used food additive. Carmine provokes both an immediate hypersensitivity and a delayed systemic response with cutaneous expression.
    Carmine
  • HY-D0069
    CPM
    		≥99.0%
    CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye (excitation/emission maxima of 387/463 nm).
    CPM
  • HY-D1836
    Vari Fluor 555 TSA (200x)
    Vari Fluor 555 TSA (200x) , also know as VF 555 Tyramide. Vari Fluor 555 TSA (200x) is one of Vari Fluor TSA series fluorescent probes, which can be used for high-density in situ labeling assays. Vari Fluor TSA series target antigens via horseradish peroxidase (HRP), which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 555 TSA (200x)
  • HY-D0988
    R-PE (R-Phycoerythrin)
    R-Phycoerythrin is found in Heterosiphonia japonica. R-Phycoerythrin is an orange-red fluorescent probe with α, β, and γ subunits. R-Phycoerythrin can be used in photodynamic therapy (PDT) to induce apoptosis in tumor cells. R-Phycoerythrin can be used in fluorescence microscopy, flow cytometry, and immunofluorescence analysis (Ex: 495 nm).
    R-PE (R-Phycoerythrin)
  • HY-108166A
    Hydroxystilbamidine bis(methanesulfonate)
    Hydroxystilbamidine bis(methanesulfonate) is a dye that can bind to DNA and RNA; it's a fluorescent cationic dye, often used as a retrograde neuronal tracer and has also been found to be a potent inhibitor of cellular ribonucleases.
    Hydroxystilbamidine bis(methanesulfonate)
  • HY-D1040
    Cy5.5-COOH
    		98.99%
    CY5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye, is commonly used in bioimaging. CY5.5-COOH shows narrow absorption spectrum, and high sensitivity and stability.
    Cy5.5-COOH
  • HY-D1805
    Vari Fluor 647-Streptavidin
    Vari Fluor 647-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=650 nm/665 nm.
    Vari Fluor 647-Streptavidin
  • HY-P1005
    Ac-DEVD-AFC
    		99.59%
    Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
    Ac-DEVD-AFC
  • HY-15937
    5(6)-FAM SE
    5(6)-FAM SE is a green fluorescent dye widely used for protein labeling.
    5(6)-FAM SE
  • HY-101882
    BCECF
    BCECF is a pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0–8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
    BCECF
  • HY-D1816
    Vari Fluor 555-Phalloidin
    Vari Fluor 555-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence when labeled, allowing for fluorescence imaging using the PE channel (Ex/Em=550 nm/561 nm).
    Vari Fluor 555-Phalloidin
  • HY-13615A
    EC-17 disodium salt
    EC-17 (disodium salt) is a folate receptor alpha (FRα) targeting contrast agent with fluorescent properties in the visible light spectrum. The peak excitation and emission wavelengths of EC-17 are 470/520 nm.
    EC-17 disodium salt
  • HY-D1122
    Janus green B
    		98.75%
    Janus green B is a supravital stain. Janus green B staining reaction is oxygen dependent, and is reversibly inhibited by cyanide. Janus green B has been used for staining peripheral nerves in live insects, lymphatic vessels of rabbits and mitochondria.
    Janus green B
Cat. No. Product Name / Synonyms Application Reactivity