2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-101895
    Fluorescein di(β-D-galactopyranoside)
    		99.81%
    Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).
    Fluorescein di(β-D-galactopyranoside)
  • HY-D1094
    Carboxy-SNARF 1 (5/6-mixture)
    Carboxy-SNARF 1 (5/6-mixture) (SNARF 1) is a fluorescent probe that is sensitive to pH (Ex: 488 nm). Carboxy-SNARF 1 (5/6-mixture) can be used for measurement pH. Carboxy-SNARF 1 (5/6-mixture) exhibits a significant emission shift from yellow-orange (Em: 580 nm) to deep red fluorescence (Em: 640 nm) under acidic and basic conditions (pH=7-8), respectively.
    Carboxy-SNARF 1 (5/6-mixture)
  • HY-136626
    Z-Ala-Ala-Asn-AMC
    		99.43%
    Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC.
    Z-Ala-Ala-Asn-AMC
  • HY-D1820
    Vari Fluor 594-Phalloidin
    Vari Fluor 594-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence at 585/609 nm when labeled.
    Vari Fluor 594-Phalloidin
  • HY-D1836
    Vari Fluor 555 TSA (200x)
    Vari Fluor 555 TSA (200x) , also know as VF 555 Tyramide. Vari Fluor 555 TSA (200x) is one of Vari Fluor TSA series fluorescent probes, which can be used for high-density in situ labeling assays. Vari Fluor TSA series target antigens via horseradish peroxidase (HRP), which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 555 TSA (200x)
  • HY-D1269
    Rhodamine-N3 chloride
    		99.85%
    Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups. Rhodamine-N3 chloride is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 544/576 nm).
    Rhodamine-N3 chloride
  • HY-D1816
    Vari Fluor 555-Phalloidin
    Vari Fluor 555-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence when labeled, allowing for fluorescence imaging using the PE channel (Ex/Em=550 nm/561 nm).
    Vari Fluor 555-Phalloidin
  • HY-D1005H
    Poloxamer 188 (F68)
    Poloxamer 188 is a nonionic linear copolymer with surfactant properties. Poloxamer 188 exhibits anti-thrombotic, anti-inflammatory, and cytoprotective activities in various tissue injury models.
    Poloxamer 188 (F68)
  • HY-D0069
    CPM
    		≥99.0%
    CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye (excitation/emission maxima of 387/463 nm).
    CPM
  • HY-136277
    DFHO
    		99.83%
    DFHO is a fluorogenic ligand of Corn fluorogenic aptamer. The RNA aptamer, Corn binds DFHO with a Kd value of 70 nM and converts it to a fluorescent form, enabling RNA imaging in cells.
    DFHO
  • HY-D0367
    Fluorescent Brightener 28
    Fluorescent brightener 28 is a fluorescent whitening agent commonly used in the padding process of the textile industry. Fluorescent brightener 28 is capable of staining polysaccharides such as cellulose, and when the plasma membrane ruptures, it also weakly stains the cytoplasm and strongly stains the cell nucleus. Additionally, Fluorescent brightener 28 can be utilized to detect intracellular chitin in living cells. Fluorescent Brightener 28 also is a visible light emitting diode (LED)-light sensitive photoinitiator for free radical photopolymerizations.
    Fluorescent Brightener 28
  • HY-D0988
    R-PE (R-Phycoerythrin)
    R-Phycoerythrin is found in Heterosiphonia japonica. R-Phycoerythrin is an orange-red fluorescent probe with α, β, and γ subunits. R-Phycoerythrin can be used in photodynamic therapy (PDT) to induce apoptosis in tumor cells. R-Phycoerythrin can be used in fluorescence microscopy, flow cytometry, and immunofluorescence analysis (Ex: 495 nm).
    R-PE (R-Phycoerythrin)
  • HY-D0042
    NBD-Cl
    		99.52%
    NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups.
    NBD-Cl
  • HY-164745
    L-β-Ethynylserine
    		99.98%
    L-β-ethynylserine (βES) is a threonine analog. L-β-ethynylserine is efficiently incorporated into newly synthesized proteins, and allows their selective visualization or enrichment through bioorthogonal ligation to fluorescent dyes or affinity tags, respectively.
    L-β-Ethynylserine
  • HY-15944
    5(6)-TAMRA
    		99.72%
    5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) is a fluorescent dye molecule widely used as a label for peptides and proteins (Ex/Em = 520/600 nm).
    5(6)-TAMRA
  • HY-125623
    MitoPerOx
    		98.14%
    MitoPerOx is a mitochondrial-targeted, lipid peroxidation-indicating fluorescent probe with BODIPY581/591 fluorophores. The triphenylphosphine cation (TPP+) of MitoPerOx can be selectively enriched in mitochondria (depending on membrane potential) and can be used to detect lipid peroxidation in the inner mitochondrial membrane. Under the action of lipid peroxides, the BODIPY581/591 fluorophores of MitoPerOx shift their emission wavelength from 590 nm (reduced state) to 520 nm (oxidized state), and ratiometric detection can be performed at an excitation wavelength of 488 nm. MitoPerOx can specifically monitor the peroxidation of mitochondrial phospholipids (especially cardiolipin) and is used in the study of oxidative stress-related diseases (such as aging, neurodegenerative diseases, and mitochondrial dysfunction)[1][2].
    MitoPerOx
  • HY-124171
    Zinquin ethyl ester
    		99.0%
    Zinquin ethyl ester is a fluorescent derivative of Zinquin and is a fluorescent probe of cytosolic zinc ion. Zinquin ethyl ester is able to penetrate cell membranes and is lipophilic and zinc-sensitive. Zinquin ethyl ester can combine with Zn2+ in the cell to produce blue fluorescence.
    Zinquin ethyl ester
  • HY-D1744
    ICG Maleimide
    ICG Maleimide is thiol reactive near infrared (NIR) fluorescent dye and used to generate a stable fluorescence signal in bioimaging (Ex/Em = 789/813 nm).
    ICG Maleimide
  • HY-B1025
    Digoxigenin
    		99.70%
    Digoxigenin (DIG) is a steroid. DIG is used for situ hybridization as a labeling molecule probe due to long shelf life and fast detection and high sensitivity of DIG-labeled riboprobes.
    Digoxigenin
  • HY-D0032
    DAF-2DA
    DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.
    DAF-2DA
Cat. No. Product Name / Synonyms Application Reactivity