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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1805
    Vari Fluor 647-Streptavidin
    Vari Fluor 647-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=650 nm/665 nm.
    Vari Fluor 647-Streptavidin
  • HY-101895
    Fluorescein di(β-D-galactopyranoside)
    		99.81%
    Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).
    Fluorescein di(β-D-galactopyranoside)
  • HY-137276
    5-Bromo-3-indolyl β-D-galactopyranoside
    		99.73%
    5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues.
    5-Bromo-3-indolyl β-D-galactopyranoside
  • HY-124171
    Zinquin ethyl ester
    		≥99.0%
    Zinquin ethyl ester is a fluorescent derivative of Zinquin and is a fluorescent probe of cytosolic zinc ion. Zinquin ethyl ester is able to penetrate cell membranes and is lipophilic and zinc-sensitive. Zinquin ethyl ester can combine with Zn2+ in the cell to produce blue fluorescence.
    Zinquin ethyl ester
  • HY-137592
    Nicotinamide 1,N6-ethenoadenine dinucleotide
    		≥99.0%
    Nicotinamide 1,N6-ethenoadenine dinucleotide (ε-NAD) , a fluorescent analogue of NAD, is able to serve as a substrate for the bacterial toxincatalyzed G-ADP ribosylation of signal-transducing G-proteins. Nicotinamide 1,N6-ethenoadenine dinucleotide can be used as a fluorescent substrate for the studies of the ADP ribosylation reaction.
    Nicotinamide 1,N6-ethenoadenine dinucleotide
  • HY-137834
    Gly-Pro-AMC hydrobromide
    		98.68%
    Gly-Pro-AMC hydrobromide is a fluorescent dye, it can be used as a specific fluorescent substrate for detecting Dipeptidyl peptidase IV (DPP-IV) activity.
    Gly-Pro-AMC hydrobromide
  • HY-15944
    5(6)-TAMRA
    		99.72%
    5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) is a fluorescent dye molecule widely used as a label for peptides and proteins (Ex/Em = 520/600 nm).
    5(6)-TAMRA
  • HY-D1816
    Vari Fluor 555-Phalloidin
    Vari Fluor 555-Phalloidin is a fluorescent derivative of Phalloidin that specifically labels myofilament proteins and exhibits red fluorescence when labeled, allowing for fluorescence imaging using the PE channel (Ex/Em=550 nm/561 nm).
    Vari Fluor 555-Phalloidin
  • HY-D1836
    Vari Fluor 555 TSA (200x)
    Vari Fluor 555 TSA (200x) , also know as VF 555 Tyramide. Vari Fluor 555 TSA (200x) is one of Vari Fluor TSA series fluorescent probes, which can be used for high-density in situ labeling assays. Vari Fluor TSA series target antigens via horseradish peroxidase (HRP), which are widely used in ELISA, IF, IHC and other techniques with high detection sensitivity.
    Vari Fluor 555 TSA (200x)
  • HY-136277
    DFHO
    		99.83%
    DFHO is a fluorogenic ligand of Corn fluorogenic aptamer. The RNA aptamer, Corn binds DFHO with a Kd value of 70 nM and converts it to a fluorescent form, enabling RNA imaging in cells.
    DFHO
  • HY-D1449
    IR-1061
    IR-1061 is a near infrared fluorescent organic dye for in vivo bimodal imaging.
    IR-1061
  • HY-101883
    BCECF-AM
    		≥99.0%
    BCECF-AM is a cell membrane permeable compound widely used as a fluorescent indicator for intracellular pH.
    BCECF-AM
  • HY-P0028
    Phalloidin
    		99.83%
    Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome .
    Phalloidin
  • HY-D0988
    R-PE (R-Phycoerythrin)
    R-Phycoerythrin is found in Heterosiphonia japonica. R-Phycoerythrin is an orange-red fluorescent probe with α, β, and γ subunits. R-Phycoerythrin can be used in photodynamic therapy (PDT) to induce apoptosis in tumor cells. R-Phycoerythrin can be used in fluorescence microscopy, flow cytometry, and immunofluorescence analysis (Ex: 495 nm).
    R-PE (R-Phycoerythrin)
  • HY-D1738
    DAPI dilactate
    		≥99.0%
    DAPI (dilactate) is a blue fluorescent dye that preferentially binds dsDNA and binds to minor groove AT clusters. DAPI (dilactate) is combined with dsDNA, and the fluorescence was enhanced about 20-fold. DAPI (dilactate) can be used to identify the cell cycle and specifically stains the nucleus but not the cytoplasm. DAPI (dilactate) form is more soluble in water than DAPI (dihydrochloride) form.
    DAPI dilactate
  • HY-D1040
    Cy5.5-COOH
    		98.99%
    Cy5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye that emits fluorescence at 710 nm upon excitation at 650 nm. Cy5.5-COOH possesses excellent spectral properties, including a narrow absorption spectrum, high sensitivity, and stability. Cy5.5-COOH can be used for bioimaging and disease diagnosis.
    Cy5.5-COOH
  • HY-D1775
    LysoTracker Blue DND-22
    LysoTracker blue DND-22 is a blue fluorescent dye (Ex/Em: 373/422 nm). LysoTracker blue DND-22 stains acidic regions in living cells. LysoTracker blue DND-22 is used in the researches of neurodegenerative diseases and leukemia.
    LysoTracker Blue DND-22
  • HY-D0069
    CPM
    		≥99.0%
    CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye (excitation/emission maxima of 387/463 nm).
    CPM
  • HY-D0032
    DAF-2DA
    DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.
    DAF-2DA
  • HY-W009756
    N-Phenylnaphthalen-1-amine
    		99.73%
    N-Phenyl-1-naphthylamine is a dye that fluoresces strongly when bound to the inner phospholipid bilayer of Gram-negative bacteria. N-Phenyl-1-naphthylamine can be used to measure outer membrane permeability. N-Phenylnaphthalen-1-amine is a fluorescence probe for odorant-binding proteins (OBP) with a dissociation constant of 1.67 μM. N-Phenylnaphthalen-1-amine exhibits an excitation wavelength of 337 nM and an emission wavelength of 407 nM.
    N-Phenylnaphthalen-1-amine
Cat. No. Product Name / Synonyms Application Reactivity