L-NIO dihydrochloride

Name: L-NIO dihydrochloride
Brand: MedChemExpress
SKU: HY-100986
Rating: 4.5 (1 reviews)

Cat. No.: HY-100986 Purity: 97.0%: Data Sheet
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Handling Instructions
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L-NIO dihydrochloride is a potent, non-selective and NADPH-dependent nitric oxide synthase (NOS) inhibitor, with K_is of 1.7, 3.9, 3.9 μM for neuronal (nNOS), endothelial (eNOS), and inducible (iNOS), respectively. L-NIO dihydrochloride induces a consistentfocal ischemic infarctin rats.

For research use only. We do not sell to patients.

L-NIO dihydrochloride Chemical Structure

CAS No. : 159190-44-0

Size	Stock	Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of L-NIO dihydrochloride:

L-NIO Get quote

1 Publications Citing Use of MCE L-NIO dihydrochloride

•Cell Mol Gastroenterol Hepatol. 2021;11(3):683-696. [Abstract]

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•Related Screening Libraries:

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View All NO Synthase Isoform Specific Products:

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

L-NIO dihydrochloride is a potent, non-selective and NADPH-dependent nitric oxide synthase (NOS) inhibitor, with K_is of 1.7, 3.9, 3.9 μM for neuronal (nNOS), endothelial (eNOS), and inducible (iNOS), respectively^[1]^[2]. L-NIO dihydrochloride induces a consistentfocal ischemic infarctin rats^[2].

IC₅₀ & Target

eNOS

iNOS

nNOS

In Vitro

L-NIO is a potent, non-selective and NADPH-dependent nitric oxide synthase (NOS) inhibitor, with K_is of 1.7, 3.9, 3.9 μM for neuronal (nNOS), endothelial (eNOS), and inducible (iNOS), respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

L-NIO (2.0 μmol, 3 days post-ischemia) causes focal cerebral ischemia in the adult rat brain^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult male Sprague Dawley rats (250-350 g)^[2]
Dosage:	0.04-2.0 μmol/(3.0-5.0 μL) per rat
Administration:	Injected into striatum 3 days post-ischemia
Result:	2.0 μmol L-NIO produced an infarct significantly different in volume from sham animals.

Molecular Weight

246.13

Formula

C₇H₁₇Cl₂N₃O₂

CAS No.

159190-44-0

Appearance

Solid-Liquid Mixture

Color

Colorless to off-white

SMILES

N[C@@H](CCCNC(C)=N)C(O)=O.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (507.86 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0629 mL	20.3145 mL	40.6289 mL
5 mM	0.8126 mL	4.0629 mL	8.1258 mL
10 mM	0.4063 mL	2.0314 mL	4.0629 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (406.29 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 97.0%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (246 KB) HNMR (257 KB)

Handling Instructions (2659 KB)

References

[1]. Babu BR, et al. N5-(1-Imino-3-butenyl)-L-ornithine. A neuronal isoform selective mechanism-based inactivator of nitric oxide synthase. J Biol Chem. 1998 Apr 10;273(15):8882-9. [Content Brief]

[2]. Van Slooten AR, et al. L-NIO as a novel mechanism for inducing focal cerebral ischemia in the adult rat brain. J Neurosci Methods. 2015 Apr 30;245:44-57. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.0629 mL	20.3145 mL	40.6289 mL	101.5723 mL
	5 mM	0.8126 mL	4.0629 mL	8.1258 mL	20.3145 mL
	10 mM	0.4063 mL	2.0314 mL	4.0629 mL	10.1572 mL
	15 mM	0.2709 mL	1.3543 mL	2.7086 mL	6.7715 mL
	20 mM	0.2031 mL	1.0157 mL	2.0314 mL	5.0786 mL
	25 mM	0.1625 mL	0.8126 mL	1.6252 mL	4.0629 mL
	30 mM	0.1354 mL	0.6771 mL	1.3543 mL	3.3857 mL
	40 mM	0.1016 mL	0.5079 mL	1.0157 mL	2.5393 mL
	50 mM	0.0813 mL	0.4063 mL	0.8126 mL	2.0314 mL
	60 mM	0.0677 mL	0.3386 mL	0.6771 mL	1.6929 mL
	80 mM	0.0508 mL	0.2539 mL	0.5079 mL	1.2697 mL
	100 mM	0.0406 mL	0.2031 mL	0.4063 mL	1.0157 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.