2. Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor Autophagy
  3. RAR/RXR Endogenous Metabolite Autophagy
  4. Isotretinoin

Isotretinoin (13-cis-Retinoic acid) is an orally active vitamin A derivative and is often be used for the research of severe acne. Isotretinoin also shows anticancer activity.

For research use only. We do not sell to patients.

Isotretinoin

Isotretinoin Chemical Structure

CAS No. : 4759-48-2

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Based on 12 publication(s) in Google Scholar

Other Forms of Isotretinoin:

Isotretinoin Related Antibodies

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Description

Isotretinoin (13-cis-Retinoic acid) is an orally active vitamin A derivative and is often be used for the research of severe acne. Isotretinoin also shows anticancer activity[1][2].

IC50 & Target[2]

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
A549 IC50
35.8 μM
Compound: 3, 13-cis-RA
Cytotoxicity against human A549 cells after 24 hrs by MTT assay
Cytotoxicity against human A549 cells after 24 hrs by MTT assay
[PMID: 19231037]
BEL-7404 tumor cell line IC50
42.6 μM
Compound: 3, 13-cis-RA
Cytotoxicity against human Bel7404 cells after 24 hrs by MTT assay
Cytotoxicity against human Bel7404 cells after 24 hrs by MTT assay
[PMID: 19231037]
HL-60 ED50
100 nM
Compound: 2
Compound was evaluated for the retinoid-induced differentiation of the human myeloid leukemia cell line HL-60 using trans-retinoic acid as the standard.
Compound was evaluated for the retinoid-induced differentiation of the human myeloid leukemia cell line HL-60 using trans-retinoic acid as the standard.
[PMID: 1992144]
In Vitro

Isotretinoin (13-cis-Retinoic acid) may act as a pro-drug that is converted intracellularly to metabolites that are agonists for RAR and RXR nuclear receptors[1].
Isotretinoin is highly sensitive to air, heat, and light[3].
Isotretinoin (0-40 μM; 24-96 h) significantly inhibits HepG2 cell viability[4].
Isotretinoin (10 μM; 48 h) down-regulates c-MYC mRNA expression and this is partially due to P1 or P2 promoter activity[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Isotretinoin Related Antibodies

Cell Viability Assay[4]

Cell Line: HepG2
Concentration: 0.1, 0.5, 1, 5, 10, 20, and 40 μM
Incubation Time: 24, 48, 72, and 96 h
Result: Showed a significant reduction in cell viability (less than 30%) at 96 h for all doses.

RT-PCR[4]

Cell Line: HepG2
Concentration: 10 µM
Incubation Time: 48 h
Result: Significantly reduced the expression of c-MYC mRNA by ~80%.
In Vivo

Isotretinoin (13-cis-Retinoic acid) (1.3-481 μg/L; inhalation exposure; 45 min daily for 2 weeks) is an effective lung cancer chemopreventive agent in A/J Mice[2].
Isotretinoin accelerates alveolar repair after exodontia in rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male A/J mice, lung carcinogenesis model[2]
Dosage: 1.3, 20.7, or 481 μg/L
Administration: Inhalation exposure, 45 min daily for 2 weeks
Result: Reduced the tumor multiplicity. Caused up-regulation of lung tissue nuclear retinoic acid receptors (RARs) relative to vehicle-exposed mice, RARα (3.9-fold vehicle), RARβ (3.3-fold), and RARγ (3.7-fold) at 20.7 μg/L.
Animal Model: Wistar rats, tooth extraction model[3]
Dosage: 7.5 mg/kg
Administration: Oral gavage, daily for 30 days
Result: Accelerated the process of alveolar repair, significantly decreased serum calcium levels.
Clinical Trial
Molecular Weight

300.44

Formula

C20H28O2
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

CC1(C)C(/C=C/C(C)=C/C=C/C(C)=C\C(O)=O)=C(C)CCC1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Purity & Documentation
References
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Isotretinoin Related Classifications

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This equation is commonly abbreviated as: C1V1 = C2V2

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× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isotretinoin4759-48-213-cis-Retinoic acidRAR/RXREndogenous MetaboliteAutophagyRetinoic acid receptorsRetinoid X receptorsvitamin Asevere acneanticancerorally activeInhibitorinhibitorinhibit

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