Iproniazid phosphate 

Iproniazid phosphate is an orally active, irreversible, non-selective monoamine oxidase (MAO) inhibitor. Iproniazid phosphate inhibits MAO activity and enhances Rotenone (HY-B1756)-induced Apoptosis. Iproniazid phosphate modulates neurotransmitter levels, affects neuronal function, induces hepatic necrosis, and interferes with the endocrine system. Iproniazid phosphate can be used in the research of depression, Parkinson's disease, and hepatotoxicity^{[1][2][3][4][5][6][7]}.

Iproniazid (10 mM; 24 h) phosphate increases intracellular dopamine content and accelerates DNA fragmentation induced by 0.1 μM Rotenone (HY-B1756) in SH-SY5Y cells^[2].
Iproniazid (100 μM; 10 min) phosphate inhibits serotonin metabolism by monoamine oxidase in cultured guinea pig tracheal smooth muscle cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Iproniazid (100 mg/kg; i.p.; 150 min before Lidocaine administration) phosphate lowers the median convulsant dose of Lidocaine in mice, reducing the seizure threshold ^[4].
Iproniazid (i.p.) phosphate causes hepatic necrosis in Sprague-Dawley and Fischer rats^[5].
Iproniazid dihydrochloride (53-224 mg/kg; p.o.; daily) phosphate retards sexual maturation, lengthens the estrous cycle, and inhibits corpus luteum formation in female rats^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

277.21

C₉H₁₆N₃O₅P

305-33-9

Solid

White to off-white

O=C(C1=CC=NC=C1)NNC(C)C.O=P(O)(O)O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Fagervall I, et al. Inhibition of monoamine oxidase in monoaminergic neurones in the rat brain by irreversible inhibitors. Biochemical pharmacology 35 (8): 1381–1387

  [2]. Watabe M, et al. Mitochondrial complex I inhibitor rotenone-elicited dopamine redistribution from vesicles to cytosol in human dopaminergic SH-SY5Y cells. J Pharmacol Exp Ther. 2007 Nov;323(2):499-507.  [Content Brief]

  [3]. Dodson AM, et al. Serotonin uptake and metabolism by cultured guinea pig airway smooth muscle cells. Pulm Pharmacol Ther. 2004;17(1):19-25.  [Content Brief]

  [4]. De Oliveira LF, et al. Effects of 5-hydroxytryptophan, iproniazid and p-chlorophenylalanine on lidocaine seizure threshold of mice. Eur J Pharmacol. 1974 Nov;29(1):5-9.  [Content Brief]

  [5]. Nelson SD, et al. Hepatotoxicity and metabolism of iproniazid and isopropylhydrazine. J Pharmacol Exp Ther. 1978 Sep;206(3):574-85.  [Content Brief]

  [6]. SETNIKAR I V O, et al. Retardation of sexual development in female rats due to iproniazid treatment. Endocrinology, 1960, 67(4): 511-520.

  [7]. Huang J T, et al. The effect of pretreatment with iproniazid on the behavioral activities of β-phenylethylamine in rats. Psychopharmacologia, 1974, 35(1): 77-81.