2. Membrane Transporter/Ion Channel
  3. Chloride Channel
  4. H100

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes.

For research use only. We do not sell to patients.

H100

H100 Chemical Structure

CAS No. : 643727-55-3

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Based on 1 publication(s) in Google Scholar

H100 Related Antibodies

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  • Purity & Documentation

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Description

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes.

IC50 & Target

Cl- transport[1]
In Vitro

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter (NKCC) and the Band 3 anion exchanger (AE), but no effect against KCl cotransporter (KCC), in human erythrocytes. H100 (0.1 mM) shows 63% and 74% inhibition of NKCC and AE in human erythrocytes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

H100 Related Antibodies
Clinical Trial
Molecular Weight

388.39

Formula

C18H16N2O6S
CAS No.
Appearance

Solid

Color

Light green to green

SMILES

O=C(O)C1=CC(N2C=CC=C2)=C(OC3=CC=C(OC)C=C3)C(S(=O)(N)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (321.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5747 mL 12.8737 mL 25.7473 mL
5 mM 0.5149 mL 2.5747 mL 5.1495 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Cell Assay
[1]

35SO42- efflux is used as a convenient measure of the activity of the Band 3 anion exchanger (AE). Erythrocytes are suspended into MBS containing Na2SO4 rather than NaCl and incubated at 37°C for 30 min. The cell suspension is then centrifuged (3,000 g, 5 min) and the supernatant removed. The above procedure is repeated twice to ensure the intracellular replacement of Cl- with SO42-. To load the erythrocytes with radiolabel, the packed cells are re-suspended to approximately 50% haematocrit in a solution containing 1 part SO42- MBS and 9 parts of a medium containing (in mM) 300 sucrose and 10 MOPS (pH 7.4, 300 ± 5 mOsm) and placed in a microcentrifuge tube. 10 μCi of 35SO42- is then added and the suspension incubated at 37°C for 1 h. At the end of this period, the cells are then washed four times by centrifugation (10,000 g, 10 s) in ice-cold SO42- MBS. All inhibitors (H100) are dissolved in either DMSO or MBS and are added to the cell suspensions prior to the addition of the radioisotope[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5747 mL 12.8737 mL 25.7473 mL 64.3683 mL
5 mM 0.5149 mL 2.5747 mL 5.1495 mL 12.8737 mL
10 mM 0.2575 mL 1.2874 mL 2.5747 mL 6.4368 mL
15 mM 0.1716 mL 0.8582 mL 1.7165 mL 4.2912 mL
20 mM 0.1287 mL 0.6437 mL 1.2874 mL 3.2184 mL
25 mM 0.1030 mL 0.5149 mL 1.0299 mL 2.5747 mL
30 mM 0.0858 mL 0.4291 mL 0.8582 mL 2.1456 mL
40 mM 0.0644 mL 0.3218 mL 0.6437 mL 1.6092 mL
50 mM 0.0515 mL 0.2575 mL 0.5149 mL 1.2874 mL
60 mM 0.0429 mL 0.2146 mL 0.4291 mL 1.0728 mL
80 mM 0.0322 mL 0.1609 mL 0.3218 mL 0.8046 mL
100 mM 0.0257 mL 0.1287 mL 0.2575 mL 0.6437 mL
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H100 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H100643727-55-3H 100H-100Chloride ChannelCl− ChannelsInhibitorinhibitorinhibit

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