2. Stem Cell/Wnt
  3. β-catenin Wnt
  4. C-82

C-82 is a second-generation specific CBP/β-catenin antagonist, which inhibits the binding between β-catenin and CBP and increases the binding between β-catenin and p300.

For research use only. We do not sell to patients.

C-82

C-82 Chemical Structure

CAS No. : 1422253-37-9

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Based on 1 publication(s) in Google Scholar

Other Forms of C-82:

C-82 Related Antibodies

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

C-82 is a second-generation specific CBP/β-catenin antagonist, which inhibits the binding between β-catenin and CBP and increases the binding between β-catenin and p300[1].

IC50 & Target

CBP/β-catenin[1]
In Vitro

C-82 is an active form of PRI-724. C-82 inhibits the activation of isolated primary mouse quiescent hepatic stellate cells (HSCs) and promotes cell death in culture-activated HSCs[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C-82 Related Antibodies
In Vivo

PRI-724 is phosphorylated-C-82 and is rapidly hydrolyzed to its active form C-82 in vivo, and pre-clinical studies have shown a very acceptable toxicity profile[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

578.66

Formula

C33H34N6O4
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(N1N(C)CC(N([C@H]2CC3=CC=C(O)C=C3)[C@]1([H])[C@H](C)N(CC4=CC=CC5=C4N=CC=C5)C2=O)=O)NCC6=CC=CC=C6
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (108.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7281 mL 8.6407 mL 17.2813 mL
5 mM 0.3456 mL 1.7281 mL 3.4563 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Cell Assay
[1]

The culture-activated hepatic stellate cells (HSCs) are treated with or without C-82 (3, 10 μM) for 5 days. Medium change is not performed after C-82 treatment. Cell viability is examined using XTT based Cell Proliferation Kit[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7281 mL 8.6407 mL 17.2813 mL 43.2033 mL
5 mM 0.3456 mL 1.7281 mL 3.4563 mL 8.6407 mL
10 mM 0.1728 mL 0.8641 mL 1.7281 mL 4.3203 mL
15 mM 0.1152 mL 0.5760 mL 1.1521 mL 2.8802 mL
20 mM 0.0864 mL 0.4320 mL 0.8641 mL 2.1602 mL
25 mM 0.0691 mL 0.3456 mL 0.6913 mL 1.7281 mL
30 mM 0.0576 mL 0.2880 mL 0.5760 mL 1.4401 mL
40 mM 0.0432 mL 0.2160 mL 0.4320 mL 1.0801 mL
50 mM 0.0346 mL 0.1728 mL 0.3456 mL 0.8641 mL
60 mM 0.0288 mL 0.1440 mL 0.2880 mL 0.7201 mL
80 mM 0.0216 mL 0.1080 mL 0.2160 mL 0.5400 mL
100 mM 0.0173 mL 0.0864 mL 0.1728 mL 0.4320 mL
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C-82 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C-821422253-37-9C82C 82β-cateninWntBeta cateninInhibitorinhibitorinhibit

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