8pyDTZ 

8pyDTZ is a pyridyl diphenylterazine (DTZ) analog and an ATP-independent pyridyl substrate of LumiLuc luciferase. 8pyDTZ exhibits spectrally shifted emission. 8pyDTZ has excellent biocompatibility and superior in vivo sensitivity. 8pyDTZ can be used for in vivo luminescence imaging^[1].

Guidelines (The following is our recommended protocol. This protocol is provided as a guide only and should be modified to suit your specific needs).
1 Working Solution Preparation ^[2]
1) Prepare a solution of 8pyDTZ in an aqueous injectable formulation containing 10% Ethanol (v/v), 10% Glycerol (HY-B1659) (v/v), 10% 2-Hydroxypropyl-β-cyclodextrin (HPBCD, w/v), and 35% PEG-300 (HY-Y0873) (v/v).
2) A typical working solution is 4 μmol (1.5 mg) 8pyDTZ in 480 μL of formulation.
Note: This product is unstable in solution. It is recommended that you prepare the solution immediately before use.
2 Specific staining steps
1) Anesthetize the mouse by isoflurane inhalation for 5 min, remove the hair in the imaging area with an electric shaver, apply Nair depilatory cream for 1 min, wipe the remaining hair with sterile gauze, rinse with DPBS and disinfect with 70% ethanol.
2) Inject 8pyDTZ working solution intraperitoneally into the left lower abdomen of the mouse.
3) Immediately place the mouse in a bioluminescence imaging system (such as the Ami HT system) and set the parameters: emission filter fully open, field of view 25 cm, aperture f-stop 1.2, merge mode 1×1, exposure time 1 s.
4) Collect bioluminescence images every 1 min for 20 min. Use Fiji (ImageJ) software to analyze the images, and use Excel for data processing.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LumiLuc-8pyDTZ pair is compared with several benchmark reporters in a tumor xenograft mouse model. LumiLuc-8pyDTZ shows high sensitivity and does not need organic cosolvents for in vivo administration. LumiScarlet-8pyDTZ is comparable to Akaluc-AkaLumine, the brightest ATP-dependent luciferase-luciferin pair, for detecting cells in deep tissues of mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

378.43

C₂₄H₁₈N₄O

2351898-91-2

Solid

Light brown to brown

O=C1C(CC2=CC=CC=C2)=NC3=C(C4=CC=NC=C4)NC(C5=CC=CC=C5)=CN31

Room temperature in continental US; may vary elsewhere.

*The compound is unstable in solutions, freshly prepared is recommended.

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

• [1]. Yeh HW, et al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965.  [Content Brief]