8-Azaadenine

Cat. No.: HY-W008550 Purity: 99.69%: Data Sheet
COA

SDS
Handling Instructions
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8-Azaadenine is an inhibitor of Xanthine Oxidase with an IC₅₀ of 0.54 μM and a K_i of 0.66 μM. 8-Azaadenine can be produced via the metabolism of 8-Azaadenosine (HY-115686) and incorporated into polynucleotide chains together with 8-Azaguanine (HY-B1468) to affect purine metabolism. 8-Azaadenine can be used in the study of cancer.

For research use only. We do not sell to patients.

8-Azaadenine Chemical Structure

CAS No. : 1123-54-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Description

In Vitro

8-Azaadenine can be synthesized into various derivatives through alkylation reactions, which exhibit antiviral activity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

136.12

Formula

C₄H₄N₆

CAS No.

1123-54-2

Appearance

Solid

Color

White to off-white

SMILES

NC1=C(N=NN2)C2=NC=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (183.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.3465 mL	36.7323 mL	73.4646 mL
5 mM	1.4693 mL	7.3465 mL	14.6929 mL
10 mM	0.7346 mL	3.6732 mL	7.3465 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (18.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (253 KB) HNMR (119 KB) RP-HPLC (222 KB)

Handling Instructions (2659 KB)

References

[1]. Sheu SY, et al. Inhibition of xanthine oxidase by synthetic cytokinin analogues. Anticancer Res. 1997 Mar-Apr;17(2A):1043-9. [Content Brief]

[2]. Holý A, et al Acyclic nucleotide analogs derived from 8-azapurines: synthesis and antiviral activity. J Med Chem. 1996 Sep 27;39(20):4073-88. [Content Brief]

[3]. Bennett LL Jr, et al. Metabolism and metabolic effects of 8-azainosine and 8-azaadenosine. Cancer Res. 1976 Nov;36(11 Pt 1):3917-23. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	7.3465 mL	36.7323 mL	73.4646 mL	183.6615 mL
	5 mM	1.4693 mL	7.3465 mL	14.6929 mL	36.7323 mL
	10 mM	0.7346 mL	3.6732 mL	7.3465 mL	18.3661 mL
	15 mM	0.4898 mL	2.4488 mL	4.8976 mL	12.2441 mL
	20 mM	0.3673 mL	1.8366 mL	3.6732 mL	9.1831 mL
	25 mM	0.2939 mL	1.4693 mL	2.9386 mL	7.3465 mL
	30 mM	0.2449 mL	1.2244 mL	2.4488 mL	6.1220 mL
	40 mM	0.1837 mL	0.9183 mL	1.8366 mL	4.5915 mL
	50 mM	0.1469 mL	0.7346 mL	1.4693 mL	3.6732 mL
	60 mM	0.1224 mL	0.6122 mL	1.2244 mL	3.0610 mL
	80 mM	0.0918 mL	0.4592 mL	0.9183 mL	2.2958 mL
	100 mM	0.0735 mL	0.3673 mL	0.7346 mL	1.8366 mL

8-Azaadenine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

8-Azaadenine1123-54-2Xanthine OxidaseXOXanthine oxidasecancerInhibitorinhibitorinhibit

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8-Azaadenine

8-Azaadenine Related Antibodies

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8-Azaadenine Related Antibodies

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