6-Methyltetrahydropterin dihydrochloride

Name: 6-Methyltetrahydropterin dihydrochloride
Brand: MedChemExpress
SKU: HY-W071686
Rating: 4.5 (1 reviews)

Cat. No.: HY-W071686 Purity: 99.64%: Data Sheet
SDS

COA
Handling Instructions
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6-Methyltetrahydropterin (dihydrochloride) is a biochemical detection reagent that can be used in tyrosine hydroxylase activity assay.

For research use only. We do not sell to patients.

6-Methyltetrahydropterin dihydrochloride Chemical Structure

CAS No. : 69113-63-9

Size	Stock	Quantity
1 mg	Get quote	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31

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Purity & Documentation
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Description

6-Methyltetrahydropterin (dihydrochloride) is a biochemical detection reagent that can be used in tyrosine hydroxylase activity assay^[1].

Molecular Weight

254.12

Formula

C₇H₁₃Cl₂N₅O

CAS No.

69113-63-9

Appearance

Solid

Color

White to off-white

SMILES

O=C1N=C(NC2=C1NC(CN2)C)N.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (196.76 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 50 mg/mL (196.76 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9351 mL	19.6757 mL	39.3515 mL
5 mM	0.7870 mL	3.9351 mL	7.8703 mL
10 mM	0.3935 mL	1.9676 mL	3.9351 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.64%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (250 KB) HNMR (421 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. J R Bostwick, et al. A tyrosine hydroxylase assay in microwells using coupled nonenzymatic decarboxylation of dopa. Anal Biochem. 1991, 192, 1. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.9351 mL	19.6757 mL	39.3515 mL	98.3787 mL
	5 mM	0.7870 mL	3.9351 mL	7.8703 mL	19.6757 mL
	10 mM	0.3935 mL	1.9676 mL	3.9351 mL	9.8379 mL
	15 mM	0.2623 mL	1.3117 mL	2.6234 mL	6.5586 mL
	20 mM	0.1968 mL	0.9838 mL	1.9676 mL	4.9189 mL
	25 mM	0.1574 mL	0.7870 mL	1.5741 mL	3.9351 mL
	30 mM	0.1312 mL	0.6559 mL	1.3117 mL	3.2793 mL
	40 mM	0.0984 mL	0.4919 mL	0.9838 mL	2.4595 mL
	50 mM	0.0787 mL	0.3935 mL	0.7870 mL	1.9676 mL
	60 mM	0.0656 mL	0.3279 mL	0.6559 mL	1.6396 mL
	80 mM	0.0492 mL	0.2459 mL	0.4919 mL	1.2297 mL
	100 mM	0.0394 mL	0.1968 mL	0.3935 mL	0.9838 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.