6,7-Dichloroquinoline-5,8-dione

Cat. No.: HY-W102931 Purity: 98.26%: Data Sheet
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6,7-Dichloroquinoline-5,8-dione (compound DA3002) is a potent CDC25B2 inhibitor with an IC₅₀ value of 4.6 µM. 6,7-Dichloroquinoline-5,8-dione has the potential for the research of cancer.

For research use only. We do not sell to patients.

6,7-Dichloroquinoline-5,8-dione Chemical Structure

CAS No. : 6541-19-1

Size	Stock	Quantity
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC₅₀: 4.6 µM (CDC25B2)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
C32	IC₅₀	22.72 μM Compound: 1	Cytotoxicity against human C32 cells harboring low levels of NQO1 after 72 hrs by WST1 assay Cytotoxicity against human C32 cells harboring low levels of NQO1 after 72 hrs by WST1 assay	[PMID: 28006669]
CEM-SS	CC₅₀	1 μM Compound: 81047	Cytotoxicity in human CEM-SS cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity in human CEM-SS cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27663546]
HEK-293T	CC₅₀	17 μM Compound: 81047	Cytotoxicity in HEK293T cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity in HEK293T cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27663546]
HFF-1	IC₅₀	15.66 μM Compound: 1	Cytotoxicity against HFF1 after 72 hrs by WST1 assay Cytotoxicity against HFF1 after 72 hrs by WST1 assay	[PMID: 28006669]
HL-60	IC₅₀	31.8 μM Compound: 7a	Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay	[PMID: 20363637]
MCF7	IC₅₀	22.5 μM Compound: 1	Cytotoxicity against human MCF7 cells harboring high levels of NQO1 after 72 hrs by WST1 assay Cytotoxicity against human MCF7 cells harboring high levels of NQO1 after 72 hrs by WST1 assay	[PMID: 28006669]
MDA-MB-231	IC₅₀	24.96 μM Compound: 1	Cytotoxicity against human MDA-MB-231 cells harboring undetectable levels of NQO1 after 72 hrs by WST1 assay Cytotoxicity against human MDA-MB-231 cells harboring undetectable levels of NQO1 after 72 hrs by WST1 assay	[PMID: 28006669]
MT4	CC₅₀	2 μM Compound: 81047	Cytotoxicity in human MT4 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity in human MT4 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 27663546]
Neutrophil	IC₅₀	1.3 μM Compound: 7a	Antiinflammatory activity in human neutrophils assessed as inhibition of PMA-induced superoxide production after 20 mins by WST1 assay Antiinflammatory activity in human neutrophils assessed as inhibition of PMA-induced superoxide production after 20 mins by WST1 assay	[PMID: 20363637]
SNB-19	IC₅₀	26.1 μM Compound: 1	Cytotoxicity against human SNB19 cells harboring undetectable levels of NQO1 after 72 hrs by WST1 assay Cytotoxicity against human SNB19 cells harboring undetectable levels of NQO1 after 72 hrs by WST1 assay	[PMID: 28006669]
T-cell	IC₅₀	32.9 μM Compound: 7a	Antiproliferative activity against human T cells after 48 hrs by MTT assay Antiproliferative activity against human T cells after 48 hrs by MTT assay	[PMID: 20363637]

Molecular Weight

228.03

Formula

C₉H₃Cl₂NO₂

CAS No.

6541-19-1

Appearance

Solid

Color

Light green to green

SMILES

C1=CN=C2C(=C1)C(=O)C(=C(C2=O)Cl)Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (109.63 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3854 mL	21.9269 mL	43.8539 mL
5 mM	0.8771 mL	4.3854 mL	8.7708 mL
10 mM	0.4385 mL	2.1927 mL	4.3854 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.26%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (254 KB) HNMR (130 KB) CNMR (129 KB) RP-HPLC (231 KB) MS (74 KB)

Handling Instructions (2659 KB)

References

[1]. Lazo JS, et al. Discovery and biological evaluation of a new family of potent inhibitors of the dual specificity protein phosphatase Cdc25. J Med Chem. 2001 Nov 22;44(24):4042-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3854 mL	21.9269 mL	43.8539 mL	109.6347 mL
	5 mM	0.8771 mL	4.3854 mL	8.7708 mL	21.9269 mL
	10 mM	0.4385 mL	2.1927 mL	4.3854 mL	10.9635 mL
	15 mM	0.2924 mL	1.4618 mL	2.9236 mL	7.3090 mL
	20 mM	0.2193 mL	1.0963 mL	2.1927 mL	5.4817 mL
	25 mM	0.1754 mL	0.8771 mL	1.7542 mL	4.3854 mL
	30 mM	0.1462 mL	0.7309 mL	1.4618 mL	3.6545 mL
	40 mM	0.1096 mL	0.5482 mL	1.0963 mL	2.7409 mL
	50 mM	0.0877 mL	0.4385 mL	0.8771 mL	2.1927 mL
	60 mM	0.0731 mL	0.3654 mL	0.7309 mL	1.8272 mL
	80 mM	0.0548 mL	0.2741 mL	0.5482 mL	1.3704 mL
	100 mM	0.0439 mL	0.2193 mL	0.4385 mL	1.0963 mL