5-AF594 tyramide 

5-AF594 tyramide is a red fluorescent dye (Ex=590 nm, Em=618 nm). 5-AF594 tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-AF594 tyramide can be used for multiplex immunohistochemistry (mIHC)^[1].

5-AF594 tyramide assay
a. Slides are permeabilized in 0.25% Triton X-100 10 min at RT.
b. Incubate overnight at 4 °C in the primary antibody.
c. Incubate in 3% H₂O₂ in 1x PBS for 20 min to block endogenous peroxidase activity.
d. Detect primary antibody with anti-rabbit IgG conjugated to FITC for 1 h at RT (1: 100), and then apply an anti-FITC-conjugated HRP antibody (1: 500) overnight at 4 °C.
e. Apply a 5-AF594 tyramide for 10 min (1:200).
f. Fluorescence microscopy detection.
Note: it is suggested that the optimal concentration of 5-FITC tyramide working solution according to actual conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

825.95

C₄₃H₄₃N₃O₁₀S₂

3046210-60-7

CN1C2=CC3=C(C(C4=C(C=C(C=C4)C(NCCC5=CC=C(C=C5)O)=O)C(O)=O)=C6C=C7C(CS([O-])=O)=CC(C)([N+](C)=C7C=C6O3)C)C=C2C(CS(=O)(O)=O)=CC1(C)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Toth ZE, et al. Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing. Exp Cell Res. 2007 May 15; 313(9):1943-50.  [Content Brief]