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  4. 5-AF594 tyramide

5-AF594 tyramide is a red fluorescent dye (Ex=590 nm, Em=618 nm). 5-AF594 tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-AF594 tyramide can be used for multiplex immunohistochemistry (mIHC).

For research use only. We do not sell to patients.

5-AF594 tyramide

5-AF594 tyramide Chemical Structure

CAS No. : 3046210-60-7

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5-AF594 tyramide Related Antibodies

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Description

5-AF594 tyramide is a red fluorescent dye (Ex=590 nm, Em=618 nm). 5-AF594 tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-AF594 tyramide can be used for multiplex immunohistochemistry (mIHC)[1].

In Vitro

5-AF594 tyramide assay
a. Slides are permeabilized in 0.25% Triton X-100 10 min at RT.
b. Incubate overnight at 4 °C in the primary antibody.
c. Incubate in 3% H2O2 in 1x PBS for 20 min to block endogenous peroxidase activity.
d. Detect primary antibody with anti-rabbit IgG conjugated to FITC for 1 h at RT (1: 100), and then apply an anti-FITC-conjugated HRP antibody (1: 500) overnight at 4 °C.
e. Apply a 5-AF594 tyramide for 10 min (1:200).
f. Fluorescence microscopy detection.
Note: it is suggested that the optimal concentration of 5-FITC tyramide working solution according to actual conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-AF594 tyramide Related Antibodies
Molecular Weight

841.95

Formula

C43H43N3O11S2
CAS No.
SMILES

O=C(C1=C(C2=C3C=C(C(CS(=O)(O)=O)=CC(C)(C)N4C)C4=CC3=[O+]C5=CC(N(C(C)(C)C=C6CS(=O)(O)=O)C)=C6C=C25)C=CC(C(NCCC7=CC=C(C=C7)O)=O)=C1)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (29.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1877 mL 5.9386 mL 11.8772 mL
5 mM 0.2375 mL 1.1877 mL 2.3754 mL
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Purity & Documentation
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Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1877 mL 5.9386 mL 11.8772 mL 29.6930 mL
5 mM 0.2375 mL 1.1877 mL 2.3754 mL 5.9386 mL
10 mM 0.1188 mL 0.5939 mL 1.1877 mL 2.9693 mL
15 mM 0.0792 mL 0.3959 mL 0.7918 mL 1.9795 mL
20 mM 0.0594 mL 0.2969 mL 0.5939 mL 1.4846 mL
25 mM 0.0475 mL 0.2375 mL 0.4751 mL 1.1877 mL
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5-AF594 tyramide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-AF594 tyramide3046210-60-7Fluorescent DyeFluorescent dyeTSARedMultiplex immunohistochemistry (mIHC)Inhibitorinhibitorinhibit

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