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  3. 4-Hydroperoxy cyclophosphamide

4-Hydroperoxy cyclophosphamide 

Cat. No.: HY-117433 Purity: ≥98.0%
COA Handling Instructions

4-Hydroperoxy cyclophosphamide is the active metabolite form of the precursor Cyclophosphamide. 4-Hydroperoxy cyclophosphamide cross-links DNA to induce T cell apoptosis independent of caspase receptor activation, and can activate the mitochondrial death pathway by producing reactive oxygen species (ROS). 4-Hydroperoxy cyclophosphamide can be used in the study of rheumatoid arthritis and autoimmune diseases.

For research use only. We do not sell to patients.

4-Hydroperoxy cyclophosphamide Chemical Structure

4-Hydroperoxy cyclophosphamide Chemical Structure

CAS No. : 39800-16-3

Size Price Stock Quantity
1 mg USD 75 In-stock
5 mg USD 171 In-stock
10 mg USD 304 In-stock
25 mg USD 700 In-stock
50 mg USD 1190 In-stock
100 mg USD 1960 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of 4-Hydroperoxy cyclophosphamide:

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

4-Hydroperoxy cyclophosphamide is the active metabolite form of the precursor Cyclophosphamide. 4-Hydroperoxy cyclophosphamide cross-links DNA to induce T cell apoptosis independent of caspase receptor activation, and can activate the mitochondrial death pathway by producing reactive oxygen species (ROS). 4-Hydroperoxy cyclophosphamide can be used in the study of rheumatoid arthritis and autoimmune diseases[1][2].

In Vitro

4-Hydroperoxy cyclophosphamide (1 or 3 μg/mL, 24, 48, 72 h) Apoptosis of Caspase-independent T cells is mediated by oxidative stress-induced mitochondrial apoptosis factor AIF and nuclear relocalization of EndoG[1].
4-Hydroperoxy cyclophosphamide (1 μg/mL, 72, 96 h) Combines with methotrexate (HY-14519), RANKL expression was inhibited in IL-6/ SIL-6R-induced fibroblast-like synoviocytes by inhibiting the JAK2/STAT3 and p38MAPK signaling pathways[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: CTL
Concentration: 3 μg/mL
Incubation Time: 24, 48, 72 h
Result: Increased the expression of p53 and Bax after 24 h.

Apoptosis Analysis[1]

Cell Line: Jurkat, CTL
Concentration: 1 or 3 μg/mL
Incubation Time: 24 , 48 , 72h
Result: Caspase-8 deficiency prevented CD95-mediated death induced by anti-CD95 monoclonal antibody (mAb) APO-1.
Overexpression of Bcl-2 inhibited apoptosis.
Induced cell death proceeded independently of caspase inhibition.
In Vivo

4-Hydroperoxy cyclophosphamide (200 mg/kg intraperitoneally injected) kills Caspase-independent T cells and B cells in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c mice[1]
Dosage: 200 mg/kg
Administration: i.p.
Result: Exhibited a marked depletion of immature double-positive CD4 þ CD8 þ thymocytes and mature single-positive CD4+ and CD8+ T cells.
Molecular Weight

293.08

Formula

C7H15Cl2N2O4P

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=P1(OCCC(N1)OO)N(CCCl)CCCl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (170.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4120 mL 17.0602 mL 34.1204 mL
5 mM 0.6824 mL 3.4120 mL 6.8241 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (8.53 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.53 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4120 mL 17.0602 mL 34.1204 mL 85.3009 mL
5 mM 0.6824 mL 3.4120 mL 6.8241 mL 17.0602 mL
10 mM 0.3412 mL 1.7060 mL 3.4120 mL 8.5301 mL
15 mM 0.2275 mL 1.1373 mL 2.2747 mL 5.6867 mL
20 mM 0.1706 mL 0.8530 mL 1.7060 mL 4.2650 mL
25 mM 0.1365 mL 0.6824 mL 1.3648 mL 3.4120 mL
30 mM 0.1137 mL 0.5687 mL 1.1373 mL 2.8434 mL
40 mM 0.0853 mL 0.4265 mL 0.8530 mL 2.1325 mL
50 mM 0.0682 mL 0.3412 mL 0.6824 mL 1.7060 mL
60 mM 0.0569 mL 0.2843 mL 0.5687 mL 1.4217 mL
80 mM 0.0427 mL 0.2133 mL 0.4265 mL 1.0663 mL
100 mM 0.0341 mL 0.1706 mL 0.3412 mL 0.8530 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
4-Hydroperoxy cyclophosphamide
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