3-Chlorophenyl boronic acid

Name: 3-Chlorophenyl boronic acid
Brand: MedChemExpress
SKU: HY-W002463
Rating: 4.5 (1 reviews)

Cat. No.: HY-W002463 Purity: 99.70%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

3-Chlorophenylboronic acid is employed as a reactant involved in 1,4-conjugate addition reactions with ethenesulfonamides to form arylethanesulfonamides, Suzuki-Miyaura reactions with dibromotrifluoromethylbenzene, cross-coupling reactions with diazoesters3 or potassium cyanate. 3-Chlorophenylboronic acid is involved in the synthesis of biarylketones and phthalides and synthesis of inhibitors including PDE4 inhibitors among others. 3-Chlorophenyl boronic acid can affect cell migration. 3-Chlorophenyl boronic acid can be used to study wound healing .

For research use only. We do not sell to patients.

3-Chlorophenyl boronic acid Chemical Structure

CAS No. : 63503-60-6

Size	Price	Stock	Quantity
25 g		In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

When the concentration of 3-Chlorophenyl boronic acid is 3.90-250 µg/mL, the cell survival rate can reach 64-109%, but when the concentration is 500 µg/mL, the survival rate drops to 17%^[1].
The cell migration effect of 3-Chlorophenyl boronic acid intensifies over time^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	L929
Concentration:	500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.90 µg/mL
Incubation Time:	22 h
Result:	Applied at a concentration of 500 µg/mL, the percentage viability rate was quite low (17.5%), but a significant increase in viability was observed at the concentration applied at 250 µg/mL, and this rate was approximately 64%. The concentration with a significant increase in viability was determined as 62.5 µg/mL (93%). The concentration that showed the highest cell viability at lower concentrations was 15.62 µg/mL (109%).

Cell Migration Assay ^[1]

Cell Line:	L929
Concentration:	15.62 µg/mL
Incubation Time:	0, 12, 24 h
Result:	There was a cell migration of 7.98 inches at the 0th hour, 5.18 inches at the 12th hour, and 4.76 inches at the 24th hour. A wound closure of 35% was detected at the 12th hour and 40% at the 24th hour.

Molecular Weight

156.38

Formula

C₆H₆BClO₂

CAS No.

63503-60-6

Appearance

Solid

Color

White to off-white

SMILES

ClC1=CC=CC(B(O)O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 200 mg/mL (1278.94 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.3947 mL	31.9734 mL	63.9468 mL
5 mM	1.2789 mL	6.3947 mL	12.7894 mL
10 mM	0.6395 mL	3.1973 mL	6.3947 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (31.97 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (31.97 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (250 KB) HNMR (120 KB) RP-HPLC (180 KB)

Handling Instructions (2659 KB)

References

[1]. Bükay Yenice Gürsu, et al. Evaluation of 2-formylphenylboronic and 3-chlorophenylboronic acid derivatives for in vitro cytotoxicity and cell migration. BORON 9(4), 173- 180, 2024.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.3947 mL	31.9734 mL	63.9468 mL	159.8670 mL
	5 mM	1.2789 mL	6.3947 mL	12.7894 mL	31.9734 mL
	10 mM	0.6395 mL	3.1973 mL	6.3947 mL	15.9867 mL
	15 mM	0.4263 mL	2.1316 mL	4.2631 mL	10.6578 mL
	20 mM	0.3197 mL	1.5987 mL	3.1973 mL	7.9933 mL
	25 mM	0.2558 mL	1.2789 mL	2.5579 mL	6.3947 mL
	30 mM	0.2132 mL	1.0658 mL	2.1316 mL	5.3289 mL
	40 mM	0.1599 mL	0.7993 mL	1.5987 mL	3.9967 mL
	50 mM	0.1279 mL	0.6395 mL	1.2789 mL	3.1973 mL
	60 mM	0.1066 mL	0.5329 mL	1.0658 mL	2.6644 mL
	80 mM	0.0799 mL	0.3997 mL	0.7993 mL	1.9983 mL
	100 mM	0.0639 mL	0.3197 mL	0.6395 mL	1.5987 mL