3,4-Dimethylpyrazole phosphate (Synonyms: 3,4-DMPP)

Cat. No.: HY-W019334 Purity: ≥98.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

3,4-Dimethylpyrazole phosphate is a nitrification inhibitor. 3,4-Dimethylpyrazole phosphate decreases gross soil autotrophic nitrification rates and reduces gross mineralization rates through feedback regulation. 3,4-Dimethylpyrazole phosphate can also reduce the risk of nitrate leaching and N losses due to denitrification and do not increase NH3 volatilization.

For research use only. We do not sell to patients.

3,4-Dimethylpyrazole phosphate Chemical Structure

CAS No. : 202842-98-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 g	In-stock	Estimated Time of Arrival: December 31
500 g	In-stock	Estimated Time of Arrival: December 31
1 k g	In-stock	Estimated Time of Arrival: December 31

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Description

Molecular Weight

194.13

Formula

C₅H₁₁N₂O₄P

CAS No.

202842-98-6

Appearance

Solid

Color

White to yellow

SMILES

CC1=CNN=C1C.O=P(O)(O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (515.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.1512 mL	25.7559 mL	51.5119 mL
5 mM	1.0302 mL	5.1512 mL	10.3024 mL
10 mM	0.5151 mL	2.5756 mL	5.1512 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (25.76 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (25.76 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (246 KB) HNMR (113 KB)

Handling Instructions (2659 KB)

References

[1]. Wang F, et, al. Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate Application During the Later Stage of Apple Fruit Expansion Regulates Soil Mineral Nitrogen and Tree Carbon-Nitrogen Nutrition, and Improves Fruit Quality. Front Plant Sci. 2020 Jun 3:11:764. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.1512 mL	25.7559 mL	51.5119 mL	128.7797 mL
	5 mM	1.0302 mL	5.1512 mL	10.3024 mL	25.7559 mL
	10 mM	0.5151 mL	2.5756 mL	5.1512 mL	12.8780 mL
	15 mM	0.3434 mL	1.7171 mL	3.4341 mL	8.5853 mL
	20 mM	0.2576 mL	1.2878 mL	2.5756 mL	6.4390 mL
	25 mM	0.2060 mL	1.0302 mL	2.0605 mL	5.1512 mL
	30 mM	0.1717 mL	0.8585 mL	1.7171 mL	4.2927 mL
	40 mM	0.1288 mL	0.6439 mL	1.2878 mL	3.2195 mL
	50 mM	0.1030 mL	0.5151 mL	1.0302 mL	2.5756 mL
	60 mM	0.0859 mL	0.4293 mL	0.8585 mL	2.1463 mL
	80 mM	0.0644 mL	0.3219 mL	0.6439 mL	1.6097 mL
	100 mM	0.0515 mL	0.2576 mL	0.5151 mL	1.2878 mL

3,4-Dimethylpyrazole phosphate Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3,4-Dimethylpyrazole202842-98-63,4-DMPPInsecticideInhibitorinhibitorinhibit

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3,4-Dimethylpyrazole phosphate (Synonyms: 3,4-DMPP)

3,4-Dimethylpyrazole phosphate Related Antibodies

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