2. Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor
  3. LXR
  4. 22(R)-Hydroxycholesterol

22(R)-Hydroxycholesterol  (Synonyms: Narthesterol)

Cat. No.: HY-116969 Purity: 99.86%
COA
SDS
Handling Instructions Technical Support

22(R)-Hydroxycholesterol (Narthesterol) is an endogenous LXR agonist. 22(R)-Hydroxycholesterol (Narthesterol) can be used for tangier disease research.

For research use only. We do not sell to patients.

22(R)-Hydroxycholesterol Chemical Structure

22(R)-Hydroxycholesterol Chemical Structure

CAS No. : 17954-98-2

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Based on 1 publication(s) in Google Scholar

Other Forms of 22(R)-Hydroxycholesterol:

22(R)-Hydroxycholesterol Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

22(R)-Hydroxycholesterol (Narthesterol) is an endogenous LXR agonist. 22(R)-Hydroxycholesterol (Narthesterol) can be used for tangier disease research[1].

Cellular Effect
Cell Line Type Value Description References
HEK293 EC50
4.75 μM
Compound: 3; 22R-HC
Activation of GAL4 fused human LXR beta expressed in HEK293 cells after 24 hrs by luciferase reporter gene assay
Activation of GAL4 fused human LXR beta expressed in HEK293 cells after 24 hrs by luciferase reporter gene assay
[PMID: 28741954]
HEK293 EC50
6.71 μM
Compound: 3; 22R-HC
Activation of GAL4 fused human LXR alpha expressed in HEK293 cells after 24 hrs by luciferase reporter gene assay
Activation of GAL4 fused human LXR alpha expressed in HEK293 cells after 24 hrs by luciferase reporter gene assay
[PMID: 28741954]
Molecular Weight

402.65

Formula

C27H46O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H](CC[C@@]12C)CC1=CC[C@@]3([C@@]2(CC[C@]4([C@]3(CC[C@]4([H])[C@H](C)[C@@H](CCC(C)C)O)[H])C)[H])[H]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : ≥ 100 mg/mL (248.35 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.86%

SDS (393 KB)

COA (248 KB) HNMR (295 KB) RP-HPLC (243 KB) MS (73 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.4835 mL 12.4177 mL 24.8355 mL 62.0887 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL 12.4177 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL 6.2089 mL
15 mM 0.1656 mL 0.8278 mL 1.6557 mL 4.1392 mL
20 mM 0.1242 mL 0.6209 mL 1.2418 mL 3.1044 mL
25 mM 0.0993 mL 0.4967 mL 0.9934 mL 2.4835 mL
30 mM 0.0828 mL 0.4139 mL 0.8278 mL 2.0696 mL
40 mM 0.0621 mL 0.3104 mL 0.6209 mL 1.5522 mL
50 mM 0.0497 mL 0.2484 mL 0.4967 mL 1.2418 mL
60 mM 0.0414 mL 0.2070 mL 0.4139 mL 1.0348 mL
80 mM 0.0310 mL 0.1552 mL 0.3104 mL 0.7761 mL
100 mM 0.0248 mL 0.1242 mL 0.2484 mL 0.6209 mL
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22(R)-Hydroxycholesterol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

22(R)-Hydroxycholesterol17954-98-2NarthesterolLXRLiver X receptorcholesteroltangier diseaseInhibitorinhibitorinhibit

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