2. Others Metabolic Enzyme/Protease
  3. Amino Acid Derivatives Phosphatase
  4. 2-Aminoacetamide hydrochloride

2-Aminoacetamide hydrochloride  (Synonyms: Glycinamide hydrochloride)

Cat. No.: HY-Y1123 Purity: ≥98.0%
COA
SDS
Handling Instructions Technical Support

2-Aminoacetamide hydrochloride (Glycinamide hydrochloride) is a derivative of Glycine (HY-Y0966). 2-Aminoacetamide hydrochloride forms cell-absorbable nanocomplexes with proteins (such as bovine serum albumin) through strong electrostatic interactions, promoting cellular uptake of related proteins. 2-Aminoacetamide hydrochloride synergizes with BMP2 to upregulate the expression of osteogenic marker genes (such as Col1a1, Alp, Runx2) and proteins (such as COL1, BSP), enhancing collagen synthesis. 2-Aminoacetamide hydrochloride synergizes with BMP2 to promote osteoblast differentiation in vitro and bone regeneration in vivo.

For research use only. We do not sell to patients.

2-Aminoacetamide hydrochloride

2-Aminoacetamide hydrochloride Chemical Structure

CAS No. : 1668-10-6

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Customer Review

Based on 1 publication(s) in Google Scholar

2-Aminoacetamide hydrochloride Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

2-Aminoacetamide hydrochloride (Glycinamide hydrochloride) is a derivative of Glycine (HY-Y0966). 2-Aminoacetamide hydrochloride forms cell-absorbable nanocomplexes with proteins (such as bovine serum albumin) through strong electrostatic interactions, promoting cellular uptake of related proteins. 2-Aminoacetamide hydrochloride synergizes with BMP2 to upregulate the expression of osteogenic marker genes (such as Col1a1, Alp, Runx2) and proteins (such as COL1, BSP), enhancing collagen synthesis. 2-Aminoacetamide hydrochloride synergizes with BMP2 to promote osteoblast differentiation in vitro and bone regeneration in vivo[1].

In Vitro

2-Aminoacetamide (1 mM; 4 days for osteogenesis markers) hydrochloride significantly enhances collagen synthesis, alkaline phosphatase (ALP) activity, and the expression of osteoblast differentiation marker genes (Col1a1, Alp, Osx, Runx2, Bsp, Ocn) in MC3T3-E1 cells under osteogenic medium (OSM) or with bone morphogenetic protein 2 (BMP2)[1].
2-Aminoacetamide (3 mg/mL; 4 h) hydrochloride readily forms submicron-sized nanocomplexes with proteins (e.g., bovine serum albumin, BSA) through strong electrostatic interactions[1].
2-Aminoacetamide (0.5-5 mM; 24-72 h) hydrochloride partially retards the proliferation of MC3T3-E1 cells and human BMSCs on day 3 but does not induce cytotoxicity in either cell line on day 1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-Aminoacetamide hydrochloride Related Antibodies
In Vivo

2-Aminoacetamide (1 M; administered via collagen sponge implantation) hydrochloride synergistically enhances bone regeneration in a mouse calvarial defect model when combined with 0.25 μg BMP2, as evidenced by increased bone volume (BV), bone volume/tissue volume ratio (BV/TV), trabecular number (Tb.N), and mature bone formation compared with BMP2 alone or other combinations[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-week-old male ICR mice with calvarial defect[1]
Dosage: 1 M Glycinamide
Administration: Administration via transplantation of Glycinamide-soaked absorbable collagen sponge into the calvarial defect
Result: Showed that the group treated with 1 M Glycinamide plus 0.25 μg BMP2 had a 65% increase in bone volume (BV) and a 64% increase in bone volume/tissue volume ratio (BV/TV) compared with the BMP2 alone group, and a 1.6-fold increase in trabecular number (Tb.N) compared with the BMP2 alone group.
Revealed that the bone area percentage of the Glycinamide plus BMP2 group was 84.7% higher than that of the BMP2 alone group.
Showed that the mature bone area percentage of this group (Glycinamide plus BMP2) was 35.2%, which was significantly higher than that of the BMP2 alone group (18.9%) and the glycine plus BMP2 group (15.5%).
Molecular Weight

110.54

Formula

C2H7ClN2O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N)CN.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (904.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 9.0465 mL 45.2325 mL 90.4650 mL
5 mM 1.8093 mL 9.0465 mL 18.0930 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (22.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (22.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.89%

SDS (393 KB)

COA (245 KB) HNMR (224 KB) RP-HPLC (239 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 9.0465 mL 45.2325 mL 90.4650 mL 226.1625 mL
5 mM 1.8093 mL 9.0465 mL 18.0930 mL 45.2325 mL
10 mM 0.9046 mL 4.5232 mL 9.0465 mL 22.6162 mL
15 mM 0.6031 mL 3.0155 mL 6.0310 mL 15.0775 mL
20 mM 0.4523 mL 2.2616 mL 4.5232 mL 11.3081 mL
25 mM 0.3619 mL 1.8093 mL 3.6186 mL 9.0465 mL
30 mM 0.3015 mL 1.5077 mL 3.0155 mL 7.5387 mL
40 mM 0.2262 mL 1.1308 mL 2.2616 mL 5.6541 mL
50 mM 0.1809 mL 0.9046 mL 1.8093 mL 4.5232 mL
60 mM 0.1508 mL 0.7539 mL 1.5077 mL 3.7694 mL
80 mM 0.1131 mL 0.5654 mL 1.1308 mL 2.8270 mL
100 mM 0.0905 mL 0.4523 mL 0.9046 mL 2.2616 mL
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2-Aminoacetamide hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Aminoacetamide1668-10-6GlycinamideAmino Acid DerivativesPhosphatasebiochemical reagentbiological materialorganic compoundlife scienceInhibitorinhibitorinhibit

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