2,4-Dihydroxybenzaldehyde

Name: 2,4-Dihydroxybenzaldehyde
Brand: MedChemExpress
SKU: HY-W007539
Rating: 4.5 (1 reviews)

Cat. No.: HY-W007539 Purity: 99.90%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

2,4-Dihydroxybenzaldehyde is an endogenous metabolite. 2,4-Dihydroxybenzaldehyde is a pharmaceutical intermediate that can be used to synthesize various Schiff base compounds. 2,4-Dihydroxybenzaldehyde exhibits significant anti angiogenic, anti-inflammatory, and analgesic activities. 2,4-Dihydroxybenzaldehyde reduces the production of NO and ROS by inhibiting the expression of iNOS and COX-2. 2,4-Dihydroxybenzaldehyde is commonly used in the study of inflammatory conditions.

For research use only. We do not sell to patients.

2,4-Dihydroxybenzaldehyde Chemical Structure

CAS No. : 95-01-2

Size	Stock	Quantity
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of 2,4-Dihydroxybenzaldehyde:

2,4-Dihydroxybenzaldehyde (Standard) Get quote

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NO Synthase Isoform Specific Products:

View All Isoforms

nNOS iNOS eNOS

View All COX Isoform Specific Products:

View All Isoforms

COX COX-1 COX-2 COX-3

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[2]

Human Endogenous Metabolite

iNOS

COX-2

In Vitro

2,4-Dihydroxybenzaldehyde (0-0.1 mM, 24 h) significantly inhibits LPS (HY-D1056) induced NO production in a dose-dependent manner in RAW264.7 cells^[2].
2,4-Dihydroxybenzaldehyde (0-0.1 mM, 24 h) inhibits LPS induced iNOS and COX-2 protein expression in a dose-dependent manner in RAW264.7 cells^[2].
2,4-Dihydroxybenzaldehyde (0-0.05 mM, 24 h) can slightly reduce the increase in ROS levels caused by LPS in RAW264.7 cells^[2].
2,4-Dihydroxybenzaldehyde (0-0.1 mM, 24 h) is non-toxic to RAW264.7 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	RAW264.7 cells
Concentration:	0, 0.02, 0.05 and 0.1 mM
Incubation Time:	24 h
Result:	Significantly reduced the levels of iNOS and COX-2.

In Vivo

2,4-Dihydroxybenzaldehyde (10-100 mg/kg, p.o., single dose) inhibits the increase of vascular permeability and exhibits analgesic activity in a dose-dependent manner in male ICR mice induced by acetic acid (HY-Y0319)^[2].
2,4-Dihydroxybenzaldehyde (0.03-0.3 mg/pouch, directly inject the airbag, single dose) significantly reduces exudate volume, neutrophil count, and nitrite content in rats with a λ-carrageenan (HY-N9470)-induced air pouch formation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Acetic acid induced male ICR mice (4 weeks, 25 g)^[2]
Dosage:	10, 30 and 100 mg/kg
Administration:	Oral administration (p.o.), single dose
Result:	Had an inhibitory effect on vascular permeability at 10 mg/kg (inhibition rate 16.7%), 30 mg/kg (inhibition rate 27.9%) and 100 mg/kg (inhibition rate 45.2%). Significantly reduced the number of twisting events.

Molecular Weight

138.12

Formula

C₇H₆O₃

CAS No.

95-01-2

Appearance

Solid

SMILES

O=CC1=CC=C(O)C=C1O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (724.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.2401 mL	36.2004 mL	72.4008 mL
5 mM	1.4480 mL	7.2401 mL	14.4802 mL
10 mM	0.7240 mL	3.6200 mL	7.2401 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (18.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (18.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (18.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (248 KB) HNMR (200 KB) RP-HPLC (104 KB)

Handling Instructions (2659 KB)

References

[1]. Murtaza S, et al. Synthesis, biological activities and docking studies of novel 2, 4-dihydroxybenzaldehyde based Schiff base. Medicinal Chemistry Research, 2016, 25(12): 2860-2871.

[2]. Jung H J, et al. Evaluation on pharmacological activities of 2, 4-dihydroxybenzaldehyde. Biomolecules & Therapeutics, 2009, 17(3): 263-269.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	7.2401 mL	36.2004 mL	72.4008 mL	181.0020 mL
	5 mM	1.4480 mL	7.2401 mL	14.4802 mL	36.2004 mL
	10 mM	0.7240 mL	3.6200 mL	7.2401 mL	18.1002 mL
	15 mM	0.4827 mL	2.4134 mL	4.8267 mL	12.0668 mL
	20 mM	0.3620 mL	1.8100 mL	3.6200 mL	9.0501 mL
	25 mM	0.2896 mL	1.4480 mL	2.8960 mL	7.2401 mL
	30 mM	0.2413 mL	1.2067 mL	2.4134 mL	6.0334 mL
	40 mM	0.1810 mL	0.9050 mL	1.8100 mL	4.5251 mL
	50 mM	0.1448 mL	0.7240 mL	1.4480 mL	3.6200 mL
	60 mM	0.1207 mL	0.6033 mL	1.2067 mL	3.0167 mL
	80 mM	0.0905 mL	0.4525 mL	0.9050 mL	2.2625 mL
	100 mM	0.0724 mL	0.3620 mL	0.7240 mL	1.8100 mL