BCDA (Synonyms: 5-bromo-4-chloroindoxyl acetate)

Name: BCDA
Brand: MedChemExpress
SKU: HY-52112
Rating: 4.5 (1 reviews)

Cat. No.: HY-52112 Purity: 99.80%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase.

For research use only. We do not sell to patients.

BCDA Chemical Structure

CAS No. : 3252-36-6

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10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE BCDA

•Anal Chem. 2025 May 22. [Abstract]

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Description

BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase^[1].

In Vitro

The reference procedures for using BCDA as a chromogenic substrate for esterase in histochemistry are as follows:
1. Tissue Processing: Rat tissues are fixed with a 4% formaldehyde + 1% calcium chloride fixative at 0-2°C for 24-48 hours, and then transferred to a 0.88 M sucrose solution (containing 9 g/L gum acacia) for 24-48 hours of osmotic treatment.
2. Preparation of Staining Solution: Mix 2 mL of 0.1 M Tris buffer (pH 8.5), 1 mL of oxidant solution (50 mM potassium ferricyanide and 50 mM potassium ferrocyanide each), 5 mL of 2 M sodium chloride solution, and 2 mL of distilled water. Quickly add the substrate ethanol solution to prepare a 0.5 mM staining solution.
3. Tissue Sectioning and Staining: Frozen sections of kidney/liver are cut at a thickness of 2-5 μm, and intercostal muscle at 15-25 μm to preserve cellular structures. The sections are transferred to the staining solution and incubated at 37°C for 5-60 minutes (prolonged incubation should be avoided to prevent diffuse dye deposition).
4. Mounting and Observation: After neutralization with a 30% ethanol-0.1% acetic acid solution, the sections are mounted with glycerol gel or counterstained, and the amorphous dye deposition at esterase-active sites can be observed under a microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

288.53

Formula

C₁₀H₇BrClNO₂

CAS No.

3252-36-6

Appearance

Solid

Color

White to off-white

SMILES

BrC1=C(Cl)C2=C(NC=C2OC(C)=O)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

Ethanol : ≥ 100 mg/mL (346.58 mM)

DMSO : 100 mg/mL (346.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4658 mL	17.3292 mL	34.6584 mL
5 mM	0.6932 mL	3.4658 mL	6.9317 mL
10 mM	0.3466 mL	1.7329 mL	3.4658 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 4

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 5

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (252 KB) RP-HPLC (248 KB)

Handling Instructions (2659 KB)

References

[1]. S J HOLT, et al. Studies in enzyme cytochemistry. V. An appraisal of indigogenic reactions for esterase localization. Proc R Soc Lond B Biol Sci. 1958 Apr 8;148(933):520-32. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.4658 mL	17.3292 mL	34.6584 mL	86.6461 mL
	5 mM	0.6932 mL	3.4658 mL	6.9317 mL	17.3292 mL
	10 mM	0.3466 mL	1.7329 mL	3.4658 mL	8.6646 mL
	15 mM	0.2311 mL	1.1553 mL	2.3106 mL	5.7764 mL
	20 mM	0.1733 mL	0.8665 mL	1.7329 mL	4.3323 mL
	25 mM	0.1386 mL	0.6932 mL	1.3863 mL	3.4658 mL
	30 mM	0.1155 mL	0.5776 mL	1.1553 mL	2.8882 mL
	40 mM	0.0866 mL	0.4332 mL	0.8665 mL	2.1662 mL
	50 mM	0.0693 mL	0.3466 mL	0.6932 mL	1.7329 mL
	60 mM	0.0578 mL	0.2888 mL	0.5776 mL	1.4441 mL
	80 mM	0.0433 mL	0.2166 mL	0.4332 mL	1.0831 mL
	100 mM	0.0347 mL	0.1733 mL	0.3466 mL	0.8665 mL

BCDA Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BCDA3252-36-65-bromo-4-chloroindoxylFluorescent DyeChromogenic substrateesteraseInhibitorinhibitorinhibit

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BCDA (Synonyms: 5-bromo-4-chloroindoxyl acetate)

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BCDA Related Antibodies

1 Publications Citing Use of MCE BCDA

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Purity & Documentation

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BCDA Related Antibodies

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Complete Stock Solution Preparation Table

BCDA Related Classifications

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