1-Oleoyl lysophosphatidic acid (Synonyms: 1-Oleoyl-sn-glycero-3-phosphate; 1-Oleoyl-LPA)

Name: 1-Oleoyl lysophosphatidic acid
Brand: MedChemExpress
SKU: HY-137862
Rating: 4.5 (1 reviews)

Cat. No.: HY-137862 Purity: 99.90%: Data Sheet
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1-Oleoyl lysophosphatidic acid (1-Oleoyl-sn-glycero-3-phosphate) is an abundant lysophosphatidic acid (LPA) species with high biological activity due to its strong affinity for the LPA receptors. 1-Oleoyl lysophosphatidic acid is commonly used in most laboratories as a reagent for LPA receptor activation. 1-Oleoyl lysophosphatidic acid increases SRE-driven β-galactosidase activity.

For research use only. We do not sell to patients.

1-Oleoyl lysophosphatidic acid Chemical Structure

CAS No. : 65528-98-5

Size	Stock	Quantity
Solvent
5 mg (22.91 mM * 500 μL in Ethanol)	In-stock	Estimated Time of Arrival: December 31
Solvent
10 mg (22.91 mM * 1 mL in Ethanol)	In-stock	Estimated Time of Arrival: December 31
Solvent
25 mg (22.91 mM * 2.5 mL in Ethanol)	In-stock	Estimated Time of Arrival: December 31

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Based on 1 publication(s) in Google Scholar

Other Forms of 1-Oleoyl lysophosphatidic acid:

1-Oleoyl lysophosphatidic acid sodium In-stock
(Rac)-1-Oleoyl lysophosphatidic acid Get quote

1 Publications Citing Use of MCE 1-Oleoyl lysophosphatidic acid

•Neurobiol Stress. 2024 Apr 4:30:100632. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

1-Oleoyl lysophosphatidic acid (1-Oleoyl-sn-glycero-3-phosphate) is an abundant lysophosphatidic acid (LPA) species with high biological activity due to its strong affinity for the LPA receptors. 1-Oleoyl lysophosphatidic acid is commonly used in most laboratories as a reagent for LPA receptor activation^[1]. 1-Oleoyl lysophosphatidic acid increases SRE-driven β-galactosidase activity^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	0.26 μM Compound: 1, LPA 18:1	Agonist activity at mouse LPA4 expressed in CHO cells by Fura-2AM dye based Ca2+ mobilization assay Agonist activity at mouse LPA4 expressed in CHO cells by Fura-2AM dye based Ca2+ mobilization assay	[PMID: 25100502]
HEK293	EC₅₀	18 nM Compound: LPA (18:1)	Agonist activity at human LPA1 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay Agonist activity at human LPA1 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay	[PMID: 23395664]
HEK293	EC₅₀	29 nM Compound: LPA (18:1)	Agonist activity at human LPA2 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay Agonist activity at human LPA2 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay	[PMID: 23395664]
HEK293	IC₅₀	442 nM Compound: LPA	Inhibition of 6x-histidine-tagged human recombinant ATX expressed in HEK293 cells using bisP-nitrophenyl phosphate as a substrate Inhibition of 6x-histidine-tagged human recombinant ATX expressed in HEK293 cells using bisP-nitrophenyl phosphate as a substrate	[PMID: 20536182]
HEK293	EC₅₀	55 nM Compound: LPA (18:1)	Agonist activity at human LPA5 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay in presence of Ki16425 Agonist activity at human LPA5 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay in presence of Ki16425	[PMID: 23395664]
HEK293	EC₅₀	77 nM Compound: LPA (18:1)	Agonist activity at human LPA3 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay Agonist activity at human LPA3 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay	[PMID: 23395664]
HEK293	EC₅₀	8.1 nM Compound: LPA (18:1)	Agonist activity at human LPA4 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay in presence of Ki16425 Agonist activity at human LPA4 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay in presence of Ki16425	[PMID: 23395664]
HEK293	EC₅₀	970 nM Compound: LPA (18:1)	Agonist activity at human LPA6 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay in presence of Ki16425 Agonist activity at human LPA6 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay in presence of Ki16425	[PMID: 23395664]
MEF	EC₅₀	0.002 μM Compound: 1, LPA 18:1	Agonist activity at human LPA2 expressed in LPA1xLPA2 double knockout mouse MEF cells by Fura-2AM dye based Ca2+ mobilization assay Agonist activity at human LPA2 expressed in LPA1xLPA2 double knockout mouse MEF cells by Fura-2AM dye based Ca2+ mobilization assay	[PMID: 25100502]
MEF	EC₅₀	0.35 μM Compound: 1, LPA 18:1	Agonist activity at human LPA1 expressed in LPA1xLPA2 double knockout mouse MEF cells up to 10 uM by Fura-2AM dye based Ca2+ mobilization assay Agonist activity at human LPA1 expressed in LPA1xLPA2 double knockout mouse MEF cells up to 10 uM by Fura-2AM dye based Ca2+ mobilization assay	[PMID: 25100502]
MEF	EC₅₀	0.83 μM Compound: 1, LPA 18:1	Agonist activity at human LPA3 expressed in LPA1xLPA2 double knockout mouse MEF cells by Fura-2AM dye based Ca2+ mobilization assay Agonist activity at human LPA3 expressed in LPA1xLPA2 double knockout mouse MEF cells by Fura-2AM dye based Ca2+ mobilization assay	[PMID: 25100502]
N1E-115	IC₅₀	10 nM Compound: oleoyl-LPA	Displacement of [32P]diazirine-LPA from LPA receptor in mouse N1E-115 cells preincubated for 1 hr in dark followed by UV irradiation for 5 mins by scintillation counting analysis Displacement of [32P]diazirine-LPA from LPA receptor in mouse N1E-115 cells preincubated for 1 hr in dark followed by UV irradiation for 5 mins by scintillation counting analysis	10.1039/C4MD00333K
RH7777	EC₅₀	0.83 μM Compound: LPA	Agonist activity at LPA1 receptor (unknown origin) stably expressed in rat RH7777 cells assessed as increase in intracellular calcium level by Fluo-4 NW dye based fluorescence assay Agonist activity at LPA1 receptor (unknown origin) stably expressed in rat RH7777 cells assessed as increase in intracellular calcium level by Fluo-4 NW dye based fluorescence assay	[PMID: 31790581]
Sf9	IC₅₀	499.8 nM Compound: 1-O-Oleoyl-LPA	Inhibition of human recombinant autotaxin expressed in insect Sf9 cells using FS-3 as substrate preincubated for 10 mins prior substrate addition measured up to 60 mins by FRET-based analysis Inhibition of human recombinant autotaxin expressed in insect Sf9 cells using FS-3 as substrate preincubated for 10 mins prior substrate addition measured up to 60 mins by FRET-based analysis	[PMID: 22460025]

In Vivo

1-Oleoyl lysophosphatidic acid (1-Oleoyl-sn-glycero-3-phosphate) (0.4-2 μg; i.c.v.; 9-week-old male Wistar rats) induces anxiety-like responses in the elevated plus maze (EPM) under novelty conditions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	9-week-old male Wistar rats^[1]
Dosage:	0.4-2 μg
Administration:	I.c.v.
Result:	Reduced open arm exploration under novelty conditions and had no effect under habituated conditions in the EPM.

Molecular Weight

436.52

Formula

C₂₁H₄₁O₇P

CAS No.

65528-98-5

Appearance

Liquid

Color

Colorless to light yellow

SMILES

CCCCCCCC/C=C\CCCCCCCC(OC[C@@H](O)COP(O)(O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (22.91 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.29 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (2.29 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (2.29 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (266 KB) SDS (393 KB)

COA (239 KB)

Handling Instructions (2659 KB)

References

[1]. Castilla-Ortega E, et al. 1-Oleoyl lysophosphatidic acid: a new mediator of emotional behavior in rats. PLoS One. 2014;9(1):e85348. Published 2014 Jan 7. [Content Brief]

[2]. Perkins LM, et al. Activation of serum response element-regulated genes by lysophosphatidic acid. Nucleic Acids Res. 1994;22(3):450-452. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2908 mL	11.4542 mL	22.9085 mL	57.2711 mL
	5 mM	0.4582 mL	2.2908 mL	4.5817 mL	11.4542 mL
	10 mM	0.2291 mL	1.1454 mL	2.2908 mL	5.7271 mL
	15 mM	0.1527 mL	0.7636 mL	1.5272 mL	3.8181 mL
	20 mM	0.1145 mL	0.5727 mL	1.1454 mL	2.8636 mL

1-Oleoyl lysophosphatidic acid Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1-Oleoyl lysophosphatidic acid65528-98-51-Oleoyl-sn-glycero-3-phosphate 1-Oleoyl-LPALPL ReceptorLysophospholipid ReceptoremotionalbehaviorserumresponsepathologicalanxietydepressionInhibitorinhibitorinhibit

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1-Oleoyl lysophosphatidic acid (Synonyms: 1-Oleoyl-sn-glycero-3-phosphate; 1-Oleoyl-LPA)

Customer Review

Other Forms of 1-Oleoyl lysophosphatidic acid:

1-Oleoyl lysophosphatidic acid Related Antibodies

1 Publications Citing Use of MCE 1-Oleoyl lysophosphatidic acid

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•Related Proteins:

View All LPL Receptor Isoform Specific Products:

Biological Activity

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References

Customer Review

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Complete Stock Solution Preparation Table

1-Oleoyl lysophosphatidic acid Related Classifications

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