1. Autophagy Neuronal Signaling
  2. PINK1/Parkin Mitophagy Amyloid-β
  3. Y040-7904

Y040-7904 is a potential mitophagy activator. Y040-7904 enhances mitophagy by promoting mitochondria transport to autophagosomes and the fusion of autophagosomes with autolysosomes. Y040–7904 induces mitophagy through the SIRT1/FoxO3 pathway. Y040-7904 reduces amyloid-β () accumulation in both in vitro and in vivo models of Alzheimer’s disease[1].

For research use only. We do not sell to patients.

Y040-7904 Chemical Structure

Y040-7904 Chemical Structure

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Description

Y040-7904 is a potential mitophagy activator. Y040-7904 enhances mitophagy by promoting mitochondria transport to autophagosomes and the fusion of autophagosomes with autolysosomes. Y040–7904 induces mitophagy through the SIRT1/FoxO3 pathway. Y040-7904 reduces amyloid-β () accumulation in both in vitro and in vivo models of Alzheimer’s disease[1].

In Vitro

Y040-7904 (20 μM, 24 h) induces a detectable increase in mitolysosomes and increases the proportions of mitophagosomes (9%) and mitolysosomes (18%), higher than those induced by the two established mitophagy inducers, Urolithin A (HY-100599) and UMI-77 (HY-18628), in HT22 cells[1].

Y040-7904 (5-20 μM, 24 h) facilitates the expression of mitophagy-related proteins PINK1 and Parkin through the SIRT1-FoxO3a pathway, ultimately resulting in an elevation in mitophagy levels in HT22 cells and N2a/APPswe cells[1].

Y040-7904 (5-20 μM, 24 h) enhances mitochondrial biogenesis by upregulateing the expression of MFN2, TIM23, and TOM20 in HT22 cells[1].

Y040-7904 (5-20 μM, 24 h) mitigates APP and Aβ1-42 accumulation in N2a/APPswe cells[1].

Y040-7904 (5-20 μM, 24 h) upregulates the levels of arkin, PINK1, and LC3II/I in N2a/APPswe cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HT22 cells, PINK1 silenced HT22 cells
Concentration: 5, 10, and 20 μM
Incubation Time: 24 h
Result: Increased the levels of mitophagy-related proteins, including PINK1, Parkin, phosphorylated Ser65-ubiquitin (pUb), and LC3B-II/LC3B–I in a dose- and time-dependent manner in HT22 cells.
Abolished the increase in mitophagy-related proteins, such as pUb and LC3 in PINK1 silenced HT22 cells.
Increased nuclear FoxO3a levels, accompanied by a concomitant decrease in cytoplasmic FoxO3a levels in HT22 cells.
Increased levels of mitochondrial marker MFN2, TIM23, and TOM20 in HT22 cells.

Western Blot Analysis[1]

Cell Line: Neuron-2a cells (N2a/APPswe cells)
Concentration: 5, 10, and 20 μM
Incubation Time: 24 h
Result: Significantly reduced the levels of APP and its C-terminal fragment C99 decreased intracellular Aβ1-42 levels in a dose-dependent manner in N2a/APPswe cells.
Significantly upregulated the levels of f Parkin, PINK1, and LC3II/I in N2a/APPswe cells.

Immunofluorescence[1]

Cell Line: HT22 cells
Concentration: 20 μM
Incubation Time: 24 h
Result: Significantly promoted the nuclear localization of FoxO3a.
In Vivo

Y040-7904 (500-1000 μM, 96 h) holds therapeutic potential for mitigating Alzheimer’s disease pathology in CL4176 and CL2006 worms[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Transgenic C. elegans strains CL417647 and CL2006[1].
Dosage: 500, 1000 μM
Administration: 96 h
Result: Resulted in a dose-dependent reduction in Aβ1-42 levels in CL4176 worms.
Decreased the paralysis rate of CL4176 worms (76.2%, 1000 μM), compared to 89.5% in untreated worm at 30 h post temperature upshift.
Reduced paralysis rate of CL2006 worms by 29.3% compared to untreated controls.
Molecular Weight

370.35

Formula

C20H18O7

SMILES

O=C1OC2=C(C=CC(OC3C(O)C(O)C(O)CO3)=C2)C(C4=CC=CC=C4)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Y040-7904
Cat. No.:
HY-174806
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