2. GPCR/G Protein
  3. Protease Activated Receptor (PAR)
  4. tcY-NH2

tcY-NH2  (Synonyms: (trans-Cinnamoyl)-YPGKF-NH2)

Cat. No.: HY-P1263 Purity: 98.85%
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tcY-NH2 ((trans-Cinnamoyl)-YPGKF-NH2) is a potent selective PAR4 antagonist peptide. tcY-NH2 inhibits thrombin- and AY-NH2-induced platelet aggregation and endostatin release, and can be used in the research of inflammation, immunology.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

tcY-NH2 Chemical Structure

tcY-NH2 Chemical Structure

CAS No. : 327177-34-4

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Based on 1 publication(s) in Google Scholar

Other Forms of tcY-NH2:

tcY-NH2 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE tcY-NH2

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Description

tcY-NH2 ((trans-Cinnamoyl)-YPGKF-NH2) is a potent selective PAR4 antagonist peptide. tcY-NH2 inhibits thrombin- and AY-NH2-induced platelet aggregation and endostatin release, and can be used in the research of inflammation, immunology[1][2][6].

IC50 & Target

PAR4

 

In Vitro

tcY-NH2 (0-500 μM) inhibits AYPGKF-NH2 (10 μM)-induced platelet (obtained from male albino Sprague–Dawley rats) aggregation, with an IC50 value of 95 μM[1].
tcY-NH2 potently activates aorta relaxation (RA) and gastric (LM) contraction, with IC50 values of 64 μM (RA) and 1 μM (LM)[1].
tcY-NH2 (Tc-YPGKF-NH2, 400 μM, 5 min) prevents endostatin release and platelet aggregation induced by thrombin or by AY-NH2[2].
tcY-NH2 (5 μM, 15 min) decreases infarct size (IS) by 51%, and increases recovery of ventricular function by 26% in an isolated heart model[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

tcY-NH2 Related Antibodies
In Vivo

tcY-NH2 (tail vein injection, 0.6 mg/kg for a single dose) alleviates liver injury in Brain death (BD) rat model, indicated by lower serum ALT/AST levels and better histomorphology[3].
tcY-NH2 (intraperitoneal injection, 0.6 mg/kg for a single dose) increases posttraumatic activation of CD4+ Tregs within the draining lymph nodes in burn injury mice model [4].
tcY-NH2 (intrapleural injection, 40 ng/kg for a single dose) inhibits neutrophil recruitment in experimental inflammation in mice[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Brain death (BD) rat model[3]
Dosage: 0.6 mg/kg for a single dose
Administration: Tail vein injection for a single dose
Result: Reduced blood platelet activation and hepatic platelet accumulation.
Attenuated the inflammatory response and apoptosis in the livers.
Inhibited the activation of NF-κB and MAPK pathways induced by Brain death (BD).
Animal Model: Burn injury model of C57BL/6 N mice[4]
Dosage: 0.6 mg/kg for a single dose
Administration: Intraperitoneal injection
Result: Increased expression and phosphorylation of PKC-θ in the presence of platelets, without affecting early posttraumatic hemostasis.
Animal Model: BALB/c mice[6]
Dosage: 40 ng/kg for a single dose
Administration: Intrapleural injection
Result: Abolished the number of rolling and adhering neutrophils on the vessel wall.
Inhibited CXCL8- and Cg-induced neutrophil migration into the pleural cavity of mice.
Molecular Weight

739.86

Formula

C40H49N7O7
CAS No.
Appearance

Solid

Color

White to off-white

Sequence Shortening

{trans-Cinnamoyl}-YPGKF-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (135.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3516 mL 6.7580 mL 13.5161 mL
5 mM 0.2703 mL 1.3516 mL 2.7032 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3516 mL 6.7580 mL 13.5161 mL 33.7902 mL
5 mM 0.2703 mL 1.3516 mL 2.7032 mL 6.7580 mL
10 mM 0.1352 mL 0.6758 mL 1.3516 mL 3.3790 mL
15 mM 0.0901 mL 0.4505 mL 0.9011 mL 2.2527 mL
20 mM 0.0676 mL 0.3379 mL 0.6758 mL 1.6895 mL
25 mM 0.0541 mL 0.2703 mL 0.5406 mL 1.3516 mL
30 mM 0.0451 mL 0.2253 mL 0.4505 mL 1.1263 mL
40 mM 0.0338 mL 0.1690 mL 0.3379 mL 0.8448 mL
50 mM 0.0270 mL 0.1352 mL 0.2703 mL 0.6758 mL
60 mM 0.0225 mL 0.1126 mL 0.2253 mL 0.5632 mL
80 mM 0.0169 mL 0.0845 mL 0.1690 mL 0.4224 mL
100 mM 0.0135 mL 0.0676 mL 0.1352 mL 0.3379 mL
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tcY-NH2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

tcY-NH2327177-34-4(trans-Cinnamoyl)-YPGKF-NH2Protease Activated Receptor (PAR)Thrombin receptorsPlateletthrombinthrombocytesinflammationimmunebrain death modelburn injury modelendostatinneutrophil migrationInhibitorinhibitorinhibit

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