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  3. TAF15 Protein, Human (His, Strep)

TAF15 Protein, Human (His, Strep) is the recombinant human-derived TAF15, expressed by E. coli , with Strep, His labeled tag. ,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

TAF15 Protein, Human (His, Strep) is the recombinant human-derived TAF15, expressed by E. coli , with Strep, His labeled tag. ,

Background

TAF15 is an RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. It binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Additionally, TAF15 can enter the preinitiation complex together with RNA polymerase II (Pol II). by similar: Other studies suggest this protein might be involved in early stages of transcriptional regulation.

Species

Human

Source

E. coli

Tag

Strep;His

Accession

Q92804 (S148-R406)

Gene ID

8148

Protein Length

Partial

Synonyms
RBP56; TAF2N
Predicted Molecular Mass
31.2 kDa
Molecular Weight

Approximately 30 kDa, based on SDS-PAGE under reducing conditions.

Purity

Greater than 80% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

TAF15 Protein, Human (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
TAF15 Protein, Human (His, Strep)
Cat. No.:
HY-P703240
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