Integrin alpha V beta 3 Protein, Human (HEK293, hFc)

Measured by its binding ability in a functional ELISA. Immobilized Human ITGAV&ITGB3, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC₅₀ of 25.9ng/ml determined by ELISA.

Human

HEK293

C-hFc

P06756(F31-V992)&P05106(G27-D718)

3685&3690

Predicted band size: 131.9 kDa; Observed band size: 140-160 kDa. (ITGAV) & 105-130 kDa. (ITGB3)

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.