Exopolyphosphatase/PPX Protein, E.coli (His-SUMO)

Exopolyphosphatase/PPX proteins are dedicated to inorganic polyphosphate (polyP) degradation, expertly releasing orthophosphate from polyphosphate termini in a sustained manner. It shows a strong preference for long-chain polyphosphates, with moderate affinity for shorter sizes (approximately 15 residues). Exopolyphosphatase/PPX Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Exopolyphosphatase/PPX protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

Exopolyphosphatase/PPX protein, specializing in the degradation of inorganic polyphosphates (polyP), proficiently releases orthophosphate in a processive manner from the termini of the polyP chain. Demonstrating a robust predilection for long-chain polyphosphates, this enzyme exhibits only modest affinity for smaller polyP sizes, typically around 15 residues. With its precision in catalyzing the breakdown of polyP, Exopolyphosphatase/PPX plays a crucial role in regulating phosphate metabolism and contributes to the dynamic balance of cellular phosphate pools.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

N-His;N-SUMO

P0AFL6 (P2-A513)

946970  [NCBI]

Full Length of Mature Protein

PIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFLKRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPILVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASGTIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLAILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQARRVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNSDLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEESTPEIAA

Approximately 74 kDa, based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.