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  3. Alleviation of neuroinflammation on electrode interface by biomimetic electrical microenvironment modulation based on collagen/polypyrrole composite film

Alleviation of neuroinflammation on electrode interface by biomimetic electrical microenvironment modulation based on collagen/polypyrrole composite film

  • Colloids Surf B Biointerfaces. 2025 Jun 17:255:114885. doi: 10.1016/j.colsurfb.2025.114885.
Chengwei Wu 1,  Xiyue Duan 1,  Xuzhao He 1,  Wenjian Weng 1,  Hang Zhou 2,  Gaorong Han 2,  Zuobing Chen 3,  Wen Chen 4,  Kui Cheng 5
Affiliations

Affiliations

  • 1 Department of Rehabilitation Medicine, the First Affiliated Hospital, School of Medicine, School of Materials Science and Engineering, National Key Laboratory of Silicon and Advanced Semiconductor Materials, Zhejiang University, Hangzhou 310027, China; Institute of Wenzhou, Zhejiang University, Wenzhou 325006, China.
  • 2 Department of Rehabilitation Medicine, the First Affiliated Hospital, School of Medicine, School of Materials Science and Engineering, National Key Laboratory of Silicon and Advanced Semiconductor Materials, Zhejiang University, Hangzhou 310027, China.
  • 3 Department of Rehabilitation Medicine, the First Affiliated Hospital, School of Medicine, School of Materials Science and Engineering, National Key Laboratory of Silicon and Advanced Semiconductor Materials, Zhejiang University, Hangzhou 310027, China. Electronic address: czb1971@zju.edu.cn.
  • 4 General Practice Department of Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, Hangzhou 310024, China. Electronic address: 13858061271@163.com.
  • 5 Department of Rehabilitation Medicine, the First Affiliated Hospital, School of Medicine, School of Materials Science and Engineering, National Key Laboratory of Silicon and Advanced Semiconductor Materials, Zhejiang University, Hangzhou 310027, China; Institute of Wenzhou, Zhejiang University, Wenzhou 325006, China. Electronic address: chengkui@zju.edu.cn.
PMID: 40543185 DOI: 10.1016/j.colsurfb.2025.114885
Abstract

Neuroinflammation associated with inflammatory activation of astrocytes on the interface of implanted neural electrodes is the main cause of electrode failure. In this work, Collagen/polypyrrole composite film (CPF) was designed and fabricated to modify the interface. The CPF had the co-exposure of Collagen and polypyrrole in morphology, which allowed the biomimetic microenvironment provided by Collagen to effectively inhibit the inflammatory activation of astrocytes. Meanwhile, the polypyrrole in the composite film significantly improved the electrochemical performance of the electrode interface, enabling it to provide an electrical microenvironment that inhibited inflammatory activation of astrocytes under electrical stimulation. Moreover, mechanistic analysis showed that appropriate electrical stimulation could upregulate calcium ion concentration while inhibit the activation of SOCE and inflammation-related signaling pathways. However, over-voltage stimulation increased ROS levels and reactivated pro-inflammatory signaling pathways. In general, benefiting from having both Collagen biochemical cues and the ability to regulate the electrical microenvironment, CPF enabled astrocytes to have the lowest inflammatory factor secretion and inflammatory marker expression under appropriate electrical stimulation, effectively inhibiting the exacerbation of neuroinflammation. This work provides a new perspective on anti-neuroinflammation of the electrode interfaces.

Keywords

Astrocytes; Collagen; Electrical stimulation; Electrode interface; Neuroinflammation.

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