2. Academic Validation
  3. Neuroprotective and antioxidant potential of papaya leaf extract and its active compounds via Nrf-2 activation

Neuroprotective and antioxidant potential of papaya leaf extract and its active compounds via Nrf-2 activation

  • Food Sci Biotechnol. 2025 Apr 2;34(11):2611-2623. doi: 10.1007/s10068-025-01873-4.
Yoon A Jeon 1 2,  Premkumar Natraj 1 2,  Ji Yong Park 3,  Seong Cheol Kim 4,  Cheon-Seok Park 5,  Young Jae Lee 1 2
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Jeju National University, Jeju, 63243 Republic of Korea.
  • 2 Veterinary Medical Research Institute, Jeju National University, Jeju, 63243 Republic of Korea.
  • 3 Department of Veterinary Physiology, BK21 FOUR Future Veterinary Medicine Leading Education & Research Center, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul National University, Seoul, 08826 Republic of Korea.
  • 4 Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeju, 63240 Korea.
  • 5 Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 17104 Republic of Korea.
PMID: 40492051 DOI: 10.1007/s10068-025-01873-4
Abstract

This study examines the antioxidant capacity of papaya leaf extract (CPL) and its efficacy in reducing oxidative stress and inflammation in neuronal cells, which are key factors in neurodegenerative diseases. Through high-performance liquid chromatography coupled with diode-array detection, we quantified phenolic compounds present in CPL. Molecular docking analyses demonstrated strong binding affinities of these phenolics to proteins involved in antioxidant, anti-inflammatory, and anti-apoptotic pathways. Functionally, CPL and its active compounds reduced intracellular Reactive Oxygen Species levels, upregulated Nrf-2/HO-1 expression, and decreased cytotoxicity in SH-SY5Y cells. Additionally, they downregulated apoptotic and inflammatory markers, highlighting its potential to modulate cellular response to oxidative stress. Furthermore, the neuroprotective effects of CPL and Vicenin-2 were significantly reduced by the Nrf-2 inhibitor, confirming their dependence on Nrf-2 signaling. These findings suggest that CPL holds significant potential as a neuroprotective agent, offering valuable insights into its mechanisms and highlighting its potential application in the prevention of neurodegenerative diseases.

Supplementary information: The online version contains supplementary material available at 10.1007/s10068-025-01873-4.

Keywords

Apoptosis; Carica papaya leaf; Inflammation; Molecular docking; Neurodegeneration; Nrf-2/HO-1 pathway; Oxidative stress.

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