2. Academic Validation
  3. Synthesis and Biological Evaluation of Disulfides Based on Garlic Extract as Type III Secretion System Inhibitors against Erwinia amylovora

Synthesis and Biological Evaluation of Disulfides Based on Garlic Extract as Type III Secretion System Inhibitors against Erwinia amylovora

  • J Agric Food Chem. 2025 May 28;73(21):12582-12590. doi: 10.1021/acs.jafc.5c01631.
Aoyun Yi 1,  Liangye Chen 1,  Junjie Wei 1,  Zhao-Sheng Zhang 2,  Wanjun Li 1,  Yu Shi 3,  Bo Wang 1,  Xiangyun Wang 4,  Zi-Ning Cui 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Green Pesticide, Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
  • 2 Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science &Technology Normal University, Nanchang 330013, China.
  • 3 College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
  • 4 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ministry of Agriculture and Rural Affairs Key Laboratory for Pesticide Residue Detection, Institute of Agro-Products Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
PMID: 40372403 DOI: 10.1021/acs.jafc.5c01631
Abstract

Fire blight, caused by Erwinia amylovora, poses a significant threat to rosaceous Plants, such as pears, apples, and hawthorns. The type III secretion system (T3SS) is a critical pathogenicity factor in the pathogenesis of E. amylovora. Disulfide compounds, including those derived from garlic extract, exhibit good bioactivity against both bacteria and fungi. In this study, we synthesized 39 disulfide compounds based on garlic extract and developed a high-throughput screening system incorporating the Bacterial luciferase lux reporter gene. Compound 5c was identified as the most effective inhibitor, significantly suppressing the promoter expression of T3SS-related genes, such as hrpA and hrpL, in E. amylovora CFBP1430. Furthermore, compound 5c inhibited the hypersensitive response (HR) triggered by E. amylovora CFBP1430 in tobacco without affecting Bacterial growth. Compound 5c also reduced the level of secretion of the pathogenic protein HrpN and diminished the pathogenicity of E. amylovora CFBP1430 in pear Infection assays. These findings offer a theoretical foundation for the development of novel T3SS inhibitors aimed at the prevention and control of fire blight disease.

Keywords

Erwinia amylovora; disulfide compounds; fire blight; inhibitors; type III secretion system (T3SS).

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