2. Academic Validation
  3. Probing the molecular mechanism of kaempferol in relieving rheumatoid arthritis based on network pharmacology

Probing the molecular mechanism of kaempferol in relieving rheumatoid arthritis based on network pharmacology

  • Sci Rep. 2025 Apr 12;15(1):12645. doi: 10.1038/s41598-025-91311-6.
Haomiao Liu # 1 2,  Huangying Lu # 3,  Xuefei Fan # 4,  Suhuan Chen 4,  Xiaoyu Chen 5,  Weilu Gao 6
Affiliations

Affiliations

  • 1 The First Clinical College of Anhui Medical University, No.81 Meishan Road, Hefei, 230032, Anhui Province, China.
  • 2 Department of Orthopedic Joint Surgery, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei, 230022, Anhui Province, China.
  • 3 Department of Transfusion Medicine, Huashan Hospital Fudan University, Shanghai, 200040, China.
  • 4 Department of Histology and Embryology, Anhui Medical University, No.81 Meishan Road, Hefei, 230032, Anhui Province, China.
  • 5 Department of Histology and Embryology, Anhui Medical University, No.81 Meishan Road, Hefei, 230032, Anhui Province, China. cxyayd@163.com.
  • 6 The First Clinical College of Anhui Medical University, No.81 Meishan Road, Hefei, 230032, Anhui Province, China. weiqiang83@163.com.
  • # Contributed equally.
PMID: 40221466 DOI: 10.1038/s41598-025-91311-6
Abstract

Kaempferol is an active compound found in traditional Chinese medicine epimedium soup, which exhibits potent anti-inflammatory and antioxidant properties. Nevertheless, the mechanism of action in rheumatoid arthritis remains unclear. This study constructed targets protein interaction networks by utilizing the String platform. The analysis of GO function and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment was performed on key target genes. Target gene validation was conducted through microarray analysis. Molecular docking was employed to evaluate the relationship between kaempferol and various key targets. In vitro experiments were conducted to elucidate kaempferol's mechanism of action on rheumatoid arthritis. Topological analysis of the protein protein interaction (PPI) network identified 10 core targets. Mitogen activated protein kinase 8 (MAPK8), Peroxisome Proliferator-activated Receptor gamma (PPARG), and nuclear factor kappa-B (NF-kB) were all differentially expressed in the microarray dataset and all belonged to the target genes of kaempferol. Furthermore, kaempferol exhibited the highest binding affinity for MAPK8. In vitro cellular experiments demonstrated that kaempferol suppressed Autophagy, and ameliorated abnormal proliferation and inflammation in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) cells by activating the MAPK8/NOD-like Receptor protein 3(NLRP3) signaling pathway.

Keywords

Fuzi Decoction; Kaempferol; MAPK8; Rheumatoid arthritis.

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