2. Academic Validation
  3. Lysophosphatidic acid receptor 3 (LPAR3) regulates ocular surface chloride transport via calcium signaling

Lysophosphatidic acid receptor 3 (LPAR3) regulates ocular surface chloride transport via calcium signaling

  • Exp Eye Res. 2025 Mar 18:255:110346. doi: 10.1016/j.exer.2025.110346.
Ethan S Lindgren 1,  Rongshan Yan 1,  Yien-Ming Kuo 1,  Qi Gao 2,  Livia de Souza Goncalves 2,  Feeling Y Chen 3,  Matilda F Chan 4,  Alan S Verkman 5,  Onur Cil 2,  Neel D Pasricha 6
Affiliations

Affiliations

  • 1 Department of Ophthalmology, University of California, San Francisco, USA.
  • 2 Department of Pediatrics, University of California, San Francisco, USA.
  • 3 Department of Cell & Tissue Biology, University of California, San Francisco, USA.
  • 4 Department of Ophthalmology, University of California, San Francisco, USA; Francis I. Proctor Foundation, University of California, San Francisco, USA.
  • 5 Departments of Medicine and Physiology, University of California, San Francisco, USA.
  • 6 Department of Ophthalmology, University of California, San Francisco, USA; Francis I. Proctor Foundation, University of California, San Francisco, USA. Electronic address: neel.pasricha@ucsf.edu.
PMID: 40112945 DOI: 10.1016/j.exer.2025.110346
Abstract

Dry eye is a multifactorial disease associated with impaired tear film homeostasis, damaging the ocular surface epithelium. Lysophosphatidic acid receptors (LPARs) are G-protein coupled receptors involved in CA2+ and cAMP signaling via PLC and adenylyl cyclase activation. LPAR activation is involved in cell proliferation and wound healing in human corneal epithelial cells (HCECs) and in neuropathic pain. This study investigates the expression and functions of LPARs in ocular surface epithelial cells. Functional measurements of ocular surface potential difference (OSPD) were done in mice with topically applied, selective LPAR modulators. LPAR3 immunostaining was performed in mouse and human cornea and conjunctiva, and mouse lacrimal gland. LPAR-induced CA2+ signaling was studied in primary and immortalized HCECs. The general LPAR agonist, linoleoyl LPA, and the LPAR3 selective agonist, 2S-OMPT, stimulated ocular surface Cl- secretion via CA2+-activated Cl- channels (CaCCs). LPAR3 was expressed in the corneal and conjunctival epithelia of mice and humans, as well as in mouse lacrimal gland. Activation of LPAR and LPAR3 in HCECs transiently elevated intracellular CA2+ through the Gq/PLC signaling pathway. LPAR3 agonists may potentially have therapeutic efficacy in ocular surface diseases, including dry eye disease.

Keywords

Calcium-activated chloride channels; Chloride transport; Dry eye disease; Intracellular calcium.

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