2. Academic Validation
  3. Inhibition of Staphylococcus aureus biofilms by poly-L-aspartic acid nanoparticles loaded with Litsea cubeba essential oil

Inhibition of Staphylococcus aureus biofilms by poly-L-aspartic acid nanoparticles loaded with Litsea cubeba essential oil

  • Int J Biol Macromol. 2023 Jul 1;242(Pt 2):124904. doi: 10.1016/j.ijbiomac.2023.124904.
Lin Lin 1,  Pin Zhang 2,  Xiaochen Chen 2,  Wei Hu 3,  Mohamed A Abdel-Samie 4,  Changzhu Li 3,  Haiying Cui 5
Affiliations

Affiliations

  • 1 School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410007, China.
  • 2 School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
  • 3 State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410007, China.
  • 4 Department of Food and Dairy Sciences and technology, Faculty of Environmental Agricultural Sciences, Arish University, El-Arish 45511, Egypt.
  • 5 School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: cuihaiying@ujs.edu.cn.
PMID: 37210052 DOI: 10.1016/j.ijbiomac.2023.124904
Abstract

Staphylococcus aureus (S. aureus) biofilms contamination on various food-contacting surfaces is considered a significant threat in the field of food. Poly-L-aspartic acid (PASP) was proven to damage biofilm by affecting Bacterial adhesion, metabolic activity, and extracellular polymeric substances in this study. Especially for eDNA, its generation was reduced by 49.4 %. After treatment with 5 mg/mL of PASP, the number of S. aureus in the biofilm at different growth stages decreased by 1.20-1.68 log CFU/mL. The nanoparticles prepared by PASP and hydroxypropyl trimethyl ammonium chloride chitosan were used to embed LC-EO (EO@PASP/HACCNPs). The results indicated that the particle size of the optimized nanoparticles was 209.84 nm with an encapsulation rate of 70.28 %. Compared to LC-EO alone, EO@PASP/HACCNPs had more significant permeation and dispersion effects on biofilms and possessed long-lasting anti-biofilm activity. For the biofilm grown for 72 h, the population of S. aureus in the EO@PASP/HACCNPs-treated biofilm was additionally reduced by 0.63 log CFU/mL compared with the LC-EO-treated group. EO@PASP/HACCNPs were also applied to different food-contacting Materials. The lowest inhibition rate of EO@PASP/HACCNPs on S. aureus biofilm still reached 97.35 %. The sensory properties of the chicken breast were not affected by EO@PASP/HACCNPs.

Keywords

Antibacterial packaging; Foodborne pathogen; Poly-L-aspartic acid.

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