2. Academic Validation
  3. Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa

Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa

  • Int J Biol Sci. 2023 Apr 17;19(7):2234-2255. doi: 10.7150/ijbs.77166.
Guillaume Martinez 1 2,  Donato Cappetta 3 4,  Marialucia Telesca 3,  Konrad Urbanek 5 6,  Giuseppe Castaldo 5 6,  Magali Dhellemmes 2,  Vincenza Grazia Mele 3,  Teresa Chioccarelli 3,  Veronica Porreca 3,  Anne-Laure Barbotin 7,  Angèle Boursier 7,  Florian Guillou 8,  Charles Coutton 1 2,  Sophie Brouillet 9,  Antonella De Angelis 3,  Liberato Berrino 3,  Riccardo Pierantoni 3,  Gilda Cobellis 3,  Rosanna Chianese 3,  Francesco Manfrevola 3
Affiliations

Affiliations

  • 1 Hôpital Couple-Enfant, Centre Hospitalier Universitaire de Grenoble, UM de Génétique Chromosomique, 38000 Grenoble, France.
  • 2 Genetic Epigenetic and Therapies of Infertility, Institute for Advanced Biosciences INSERM U1209, CNRS UMR5309, 38000 Grenoble, France.
  • 3 Department of Experimental Medicine, University of Campania L. Vanvitelli, via Costantinopoli 16, 80138, Naples, Italy.
  • 4 Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce.
  • 5 Department of Molecular Medicine and Medical Biotechnologies, University of Naples "Federico II", Via A. Pansini 5, 80131 Naples, Italy.
  • 6 CEINGE-Advanced Biotechnologies, Via G. Salvatore 486, 80131 Naples, Italy.
  • 7 CHU Lille, Institute de Biologie de la Reproduction-Spermiologie-CECOS, F-59000, Lille, France.
  • 8 CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly, France.
  • 9 Université de Montpellier, EmbryoPluripotency, DEFE, INSERM 1203, Hôpital Arnaud de Villeneuve, CHRU Saint-Eloi, 80 Avenue Augustin Fliche, CEDEX 05, 34295 Montpellier, France.
PMID: 37151878 DOI: 10.7150/ijbs.77166
Abstract

In spermatozoa, the nuclear F-actin supports the acroplaxome, a subacrosomal structure involved in the correct exposure of several acrosomal membrane proteins; among them, the glycoprotein IZUMO1 is the major protein involved in sperm-oocyte fusion. Nuclear F-actin is also involved in sperm head shaping and chromosome compartmentalization. To date, few notions regarding the bivalent role of F-actin on sperm chromatin organization and IZUMO1 positioning have been reported. In our work, we characterized subcellular organization of F-actin in human high- and low-quality spermatozoa (A- and B-SPZ), respectively, showing that F-actin over-expression in sperm head of B-SPZ affected IZUMO1 localization. A correct IZUMO1 repositioning following in vitro induction of F-actin depolymerization, by cytochalasin D treatment, occurred. Interestingly, F-actin depolymerization was also associated with a correct acrosome repositioning, thus to favor a proper acrosome reaction onset, with changes in sperm nuclear size parameters and histone acetylation rate reaching high-quality conditions. In conclusion, the current work shows a key role of F-actin in the control of IZUMO1 localization as well as chromatin remodeling and acetylation events.

Keywords

F-actin; IZUMO1; chromosomes territories; histone acetylation; sperm quality.

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